RESUMO
OBJECTIVE@#To explore the mechanism of Pi (Spleen)-deficiency-induced functional diarrhea (FD) model rats treated by Shenling Baizhu Powder (, SBP).@*METHODS@#Thirty male Sprague-Dawley rats were randomly divided into 5 groups including control, model, low-, medium-, and high-dose SBP groups (SBPLDG, SBPMDG, SBPHDG), 6 rats in each group, respectively. Pi-deficiency-induced FD rats model was developed through Radix et Rhizoma Rhei gavage for 7 days. After modeling, the rats were treated with 3 doses of SBP [0.93, 1.86, and 3.72 g/(kg·d)], and the rats in the control and model groups were given pure water for 7 days. The diarrhea index was calculated. On the 7th and 14th days, the traveled distance of rat was measured by the open field test. Serum D-xylose content was determined by the phloroglucinol method and interleukin (IL)-10 and IL-17 levels were measured using an enzyme-linked immunosorbent assay kit. The content of Treg cells was determined by flow cytometry.@*RESULTS@#Compared with the control group, the diarrhea index and IL-17 level in the model group were significantly higher and the total exercise distance and D-xylose content significantly decreased (P>0.05). The expression of IL-10 in the SBPHDG group was significantly up-regulated, and serum D-xylose level and Treg cells increased significantly compared with the model group (P>0.05).@*CONCLUSION@#High-dose SBP exhibited ameliorating effects against Pi-deficiency induced FD, which might be attributed to its modulations on intestinal absorption function as well as adaptive immunity in mesenteric lymph nodes of rat.
RESUMO
The intestinal mucosa is the biggest lymph organ in human body, which consists of the first line of defensive system with the respiratory mucosa and urinary mucosa. The intestinal mucosa is responsible to defend bacteria, viruses, dietary pathogens, non-steroid anti-inflammatory drug and so on. If the intestinal mucosal immune system is destroyed, the human will suffer many diseases such as infectious diseases,autoimmune diseases. Therefore, it plays an important part in physical health. T helper type 17 (Th17) cells different from Th1 cells and Th2 cells are a distinct lineage of T cells. The discovery of Th17 cells has ruined the constitutional pattern of Th1/Th2. Th17 cells, which are important parts of intestinal mucosal immune system,play critical roles in intestinal autoimmune diseases, tissue inflammation and the infection of various pathogenic bacteria. This paper will review the differentiation of Th17 cell and its function in intestinal mucosal barrier,intestinal autoimmune diseases and intestinal infectious diseases.