Effect of crocin on the expression of VEGF-C in the human bladder cancer celI lines T24 / 重庆医科大学学报

Objective:To study the effect of Crocin on the expression of VEGF-C in transitional cell carcinoma of bladder (TCCB) T24 cell. Methods:T24 cells were treated with Crocin. MTT assay was adopted to determine the inhibition rate for selecting the best effect time and concentration of Crocin. The reverse transcription polymerase chain reaction(RT-PCR) and cDNA microarray were used to evaluate VEGF-C mRNA expression of T24 cells at around the best effect time and as the Crocin concentration reached the best. Results:The growth of T24 cells was inhibited significantly(P

Efects of PLCepsilon siRNA gene transfection on invasive power of human bladder cancer cell lines T24 / 重庆医科大学学报

Objective:To study the effects of PLCepsilon siRNA transfection on invasive power of human bladder cancer cell lines T24,and to bring a new method of gene therapy for bladder cancer.Methods:An eukaryotic expression vector containing PLCepsilon siRNA was transfected into T24 and positive cell clones were selected and amplified.Expression of PLCepsilon mRNA was detected by RT-PCR.invasive power of T24 were measured before and after transfection by the membrane invasion culture system(Transwell chamber)and gelatin enzymography(including 72 ku typeIV collagenase and 92 ku typeIVcolagenase analyzed).Results:the result of RT-PCR display that the T24 transfected with pll-PLCepsilon and p12-PLCepsilon vectors had lower expressing of PLCepsilon mRNA than the control groups T24 transfected with HK-A and T24 without transfection.In the membrane invasion culture system the invasion number of T24 transfected with p12-PLCepsilon(26.8?5.8)and the number of T24 transfected with pll-PLCepsilon(25.8?6.2)are both lower than the the control groups T24 transfected with HK-A(33.8?5.7)and T24 without transfection(34.8?6.9)(P