RESUMO
Aim To observe the effects of the total fla-vonoids of scutellaria barbataon ( TFSB ) on high-fat feeding ApoE gene deficiency mice in early atheroscle-rosis ( AS ) and its underlying mechanisms. Methods 40 ApoE-/ -male mice were divided into five groups:model group, SIM group and L-TFSB, M-TFSB, H-TFSB group, 5 C57BL/6J mice were selected as nor-mal control group. All mice in experimental group were fed with high-lipid diet for 4 weeks and all mice were killed after 8 weeks. H&E staining was used to observe morphology of aorta. Blood rheometer was used to ex-amine plasm viscosity and whole blood viscosity. Fully automatic biochemical analyser was used to detect the serum levels of TG, TC, LDL-C and HDL-C. The ex-pression levels of PLTP and VE in serum were meas-ured by ELISA. The expression levels of PLTP and FXR in liver were examined by Western blot. Results The model was established successfully. TFSB groups could improve the aorta AS morphology of model mice and significantly reduce the serum levels of TG, TC and LDL-C, while increase the level of HDL-C ( P hematocrit value, plasma viscosity and whole blood vis-cosity of AS model mice significantly and had statistical significance when compared with model group ( P <0. 01 ) . The expression levels of PLTP of serum were reduced significantly when compared with model group ( P <0. 01 ) . We also found that the expression of PLTP was in negative correlation with VE ( r = -0. 675,P<0. 01). M-TFSB and H-TFSB group could decrease the expressions of PLTP and FXR of liver when compared with model group ( P <0. 01 ) . Con-clusion TFSB may exert its anti-AS effect partly through inhibiting the levels of FXR and PLTP of ApoE-/ -mice, increasing the level of VE, regulating blood lipids, improving blood rheology and reducing the damage of AS in mice.
RESUMO
Objective To observe the influence of total flavonoids from Scutellaria baicalensis (TFSB) on Bleomycin-induced pulmonary fibrosis in rats and investigate the relative mechanism. Methods The pulmonary fibrosis model was established by intratracheal injection of Bleomycin. After 28 d, rats with TFSB treatment by ig administration once a day were killed. The levels of T-AOC, GSH, and MPO in serum were measured and analyzed and the fixed parts of the lung tissue were obtained for HE and Masson staining for histopathological examination. The influences of TFSB on the expression of transforming growth factor-β1 (TGF-β1), Smad2, Smad7, α-SMA, and Collagen I mRNA in lung tissue of rats with Bleomycin-induced pulmonary fibrosis were assayed by RT-PCR. Results Compared with the model group, the levels of T-AOC and GSH in serum were dramatically increased and the level of MPO was decreased remarkably after TFSB treatment; The alveolitis and fibrosis extent were attenuated after TFSB treatment (P< 0.05, 0.01), and the expression levels of TGF-β1, Smad2, α-SMA, and Collagen I mRNA were decreased, and the expression level of Smad7 mRNA was increased (P<0.05, 0.01) significantly. Conclusion TFSB could have mitigable effect on Bleomycin-induced pulmonary fibrosis in rats. The mechanisms might be related to the anti-oxidative damage, the inhibition of activation of inflammatory cell infiltration, and the regulation of TGF-β1/Smad signal pathway.