Fator de Necrose Tumoral Alfa eGravidade Clínica em Primoinfectadospelo Plasmodium Vivax / Tumor Necrosis Factor Alpha and Clinical Severity in Non-Immune Plasmodium Vivax

Avaliar a relação entre o FNT-α, a gravidade daapresentaçمo clيnica e a trombocitopenia, além da freqüênciado FNT2 e sua influência sobre os nيveis da citocina. Materiale Métodos: Foram avaliados pacientes primoinfectados peloP. vivax, ³ 15 anos, provenientes de Belém, Parل, com sintomashل £ 21 dias, atendidos e acompanhados clinicamente noInstituto Evandro Chagas (IEC) ou hospitalizados. A parasitemiafoi quantificada todos os dias até a negativaçمo. Adotou-se acloroquina e a primaquina por via oral, durante sete dias,como tratamento. Amostras sanguيneas foram coletadas emD0 e D7 para anلlises hematolَgicas e bioquيmicas, tيtulos doFNT-α e a freqüência do FNT2. Os programas Biostat 2.0 eExcel-Windows foram utilizados para as anلlises estatيsticasdas diferenças e das correlaçُes entre os parâmetros obtidos.Resultados: Oitenta e três pacientes foram atendidos eacompanhados clinicamente no D0, D1, D2, D3 e D7, defevereiro/2002 a março/2003. O FNT-α se correlacionoupositivamente e significativamente com a parasitemia (rs =0,33; p < 0,01) e com os escores clيnicos (rs = 0,48; p = 0,0001),porém negativamente com as plaquetas (rs = -0,48; p < 0,001)em D0. Os nيveis elevados de FNT-a foram responsلveis pelaintensidade das manifestaçُes clيnicas e pela trombocitopenianestes pacientes, porém o FNT2 nمo produziu tيtulos altos dacitocina. Conseqüentemente, tيtulos elevados de FNT-a forambenéficos na maioria dos casos, a plaquetopenia é um sinalde gravidade e outros fatores genéticos foram responsلveispelos nيveis elevados dessa citocina nesta amostra...

Evaluate the relationship between the TNF-a, theseverity of clinical presentation, the thrombocytopenia, thefrequency of TNF2 and its influence over cytokine levels.Material e Methods: We evaluated patients with first episodeof vivax malaria, ³ 15 years, with disease duration £ 21 days,coming from the city of Belém, Parل, attended at the InstitutoEvandro Chagas (IEC) or hospitalized. The parasitemia wasquantified every day till the negativation. The adoptedtreatment was cloroquine and primaquine PO, during sevendays. Blood samples were collected at D0 and D7 to realizequantification of hematological and biochemical parameters,TNF-a titles and TNF2 frequency. The programs Biostat 2.0and Excel for Windows were applied to analyze the differencesand correlations between the parameters. Results: Eighty threepatients were attended and accompanied clinically at D0, D1,D2, D3 and D7, from february/2002 till march/2003. TheTNF-a level correlated positively and significantly withparasitemia (rs = 0.33, p < 0.01) and clinical scores (rs = 0.48,p = 0.0001), but negatively with the platelets (rs = -0.48, p <0.0001) at D0. Consequently, high titles of TNF-a levels werebeneficial to the majority of the cases; the thrombocytopeniais a signal of severity, and others genetic factors were responsiblefor the high levels of this cytokine and phenotypicmanifestations on this sample...

Relation Between Level of CA125 and Heart Function as well as Myocardial Remodeling in Patients with Congestive Heart Failure / 医学研究杂志

Objective To study the relation between the level of serum carbohydrate antigen-125 (CA125) and heart function as well as the myocardial remodeling in patients with congestive heart failure(CHF). Methods 79 patients with CHF were divided into three groups based on the standard of New York Heart Association Classification. 25 healthy persons were served as control group. Level of CA125 was measured by MEIA. NT-proBNP was detected by Roche Cardiac Reading instrument. TNF-? was measured by radioimmunoassay. Left ventricular mass index (LVMI),left atrial volume index (LAVI) and left ventricular ejection fraction (LVEF) were detected by echocardiography. The levels of CA125,NT-proBNP,TNF-?,LVMI,LAVI and LVEF in different groups were compared.The relationship between CA125 and NT- proBNP,TNF-?,LVMI,LAVI and LVEF was evaluate.Results Levels of CA125,NT-proBNP,TNF-?,LVMI and LAVI in patients with CHF were much higher than those without CHF. The LVEF was much lower in NYHA Ⅲ and NYHA Ⅳ group than that in without CHF and NYHAⅠgroup. Conclusion CA125 was positively associated with the NT-proBNP,TNF-? and LVMI.

The Role of TNFalpha Gene Promoter Polymorphism in the Development of Coal Workers' Pneumoconiosis / 대한산업의학회지

OBJECTIVES: This study was performed in order to investigate the frequency of the TNF2 allele in patients with coal workers pneumoconiosis (CWP). METHODS: We compared the genotype distribution of TNFalpha gene promoter polymorphism between 80 CWP patients and 54 healthy controls. RESULTS: The results were as follows : 1. The rare allele TNF2 was significantly more frequent in CWP patients (20.6 %) than in controls (10.2 %). 2. The spontaneous or LPS-induced release of TNFalpha from the peripheral monocytes was slightly increased in the TNF2 group, but these values were not significantly different between groups. 3. In the CWP TNF2 group, the increase of LPS-induced TNFalpha release was significant in comparison with that of the controls. CONCLUSIONS: From the above results, we suggest that the TNF2 allele is strongly associated with susceptibility to CWP development.