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@#Cestode infections is widely reported in rodents, however species identification remains problematic due to the genetic or interspecies variation. Therefore, this study was aimed to verify the Cyclophyllidean parasites recovered from wild rats captured from different forest types using molecular based methods. Maximum-likelihood (ML) and neighbour-joining (NJ) trees were constructed inferred from 18 small subunit nuclear ribosomal RNA gene (18SrDNA) and mitochondrial cytochrome c oxidase subunit one gene (COX1) sequences of cestode worms recovered from 124 individuals from four rat species. Sequences obtained from both Hymenolepis diminuta and Hydatigera parva represents the first records in Malaysia. All the sequences were successfully amplified with product with total length of 205 and 1202 base pairs (bp), respectively. Three cestode species from the Family Hymenolepididae (Hymenolepis diminuta) and Family Taeniidae (Hydatigera parva; Hydatigera taeniaeformis) were successfully characterized using phylogenetic analyses and haplotype networking. Phylogenetic analysis showed that H. diminuta, Hydatigera parva (Hy. parva) and Hydatigera taeniaeformis (Hy. taeniaeformis) formed its own monophyletic clade in 18SrDNA analyses. Results also showed that Hy. taeniaeformis shared the same haplotype group with Hy. taeniaeformis from China (COX1) and linked with Hy. taeniaeformis from Japan (18SrDNA) while the Malaysian H. diminuta clearly formed a separate haplotype and networked with other regions. The Malaysian Hy. parva isolation, on the other hand, appeared to be genetically distinct from the European Hy. parva (Spain) strain, but closely linked to the local isolates. Molecular methods employed successfully improved in the detection of complex species in this group. The findings showed that molecular data can be useful to deeply study intra-specific variation in other cestode worms.
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Serine protease inhibitor, a protein superfamily that inhibits the serine protease activity, protects hosts from parasitic infections. This review describes the spatial structure and classification of serine protease inhibitor, mechanisms underlying the interplay between serine protease inhibitor and host immune responses and current advances in serine protease inhibitor of zoonotic cestode family Taeniidae, so as to provide insights into the diagnosis of zoonotic tapeworm infections, discovery of therapeutic targets and screening of vaccine candidates.
RESUMO
En el presente trabajo se describe metacestodos colectados del ratón orejón de ancas amarillas (Phyllotis xanthopygus) provenientes del distrito de Marangani en Cusco, Perú. Un total de 6 metacestodos fueron estudiados e identificados como fimbriocercos de Taenia sp. Los fimbriocercos presentaron un cuerpo ligeramente alargado y esférico, con una masa de tejido denso y de color blanquecino lechoso. Cada fimbriocerco presento un escólex invaginado el cual tenía 4 ventosas y un róstelo armado con 22 a 25 pares de ganchos. El estudio demuestra que el ratón P. xanthopygus participa como hospedero intermediario para una especie de Taenia en la sierra sur del Perú.
Metacestodes were collected from the peritoneal cavity of the yellow-rumped leaf-eared mouse (Phyllotis xanthopygus) from the Marangani district in Cuzco, Peru. A total of 6 metacestodes were studied and identified as fimbriocercus of Taenia sp. The fimbriocercus had a slightly elongated and spherical body, with a mass of dense tissue and whitish milky color. Each fimbriocercus had an invaginated scolex which had 4 suckers and a rostellum armed with 22 to 25 pairs of hooks. This study shows that the mouse P. xanthopygus participates as an intermediate host for a Taenia species in the southern highlands of Peru.