Research Progress of Retention Index Principle in Rapid Screening and Quantitative Analysis of Pesticide Residues in Chinese Herbal Medicines / 中国药学杂志

The pesticide residues in traditional Chinese herbal medicines have seriously affected its safety of quality and international reputation, and have been widely concerned by the public and regulatory authorities. With the increasing demand for high-quality green Chinese medicine products, the frequency of quantitative determination of pesticide residues in traditional Chinese medicine products by the regulatory authorities will also increase significantly. It is imperative to establish a series of quantitative methods for pesticide residue analysis that are fast, simple, high-throughput, and low-cost. This paper mainly summarized the research status of pesticide residues analysis in Chinese herbal medicines, summarized the difficulties encountered in the analysis of pesticide residues in Chinese herbal medicines, and introduced the advantages of the retention index principle applied to the analysis of pesticide residues in Chinese herbal medicines. This paper highlights the research progress of retention index principle combined with the Smart Database-Pesticides database applied to three exemplary examples, including: the quantitative determination of 12 pyrethroid pesticide compounds in Anisi Stellati Fructus, the quantitative determination of 74 pesticide compounds in Chuanxiong Rhizoma, and quantitative determination of 130 pesticide compounds in Panacis Quinquefolii Radix. The purpose of this paper is to make retention index principle more popular in the rapid analysis of pesticide residues in traditional Chinese herbal medicines.

Establishment of dual reverse phase chromatography combined parallel reaction monitoring for targeted quantitative proteomics / 生物工程学报

Steady improvement in mass spectrometers technology has transformed the targeted proteome analysis into a new stage. Parallel reaction monitoring (PRM) technology has evolved from the basic multiple reaction monitoring (MRM) targeted proteomics methods in recent years. PRM performs with a higher sensitivity, throughput and reproducibility in targeted quantification, however its limitations in effectiveness and accurate quantification of samples with higher complexity still remain unsolved. In this study through improving the chromatographic conditions of PRM we established a simple and robust platform for targeted proteomic quantification. The newly established PRM system is equipped with columns with increased inner diameter (150 μm) and decreased total length (8 cm); faster liquid phase elution rate (800 nL/min) and shortened elution gradient (35 min). These modifications enable PRM platform to combine with dual reverse phase chromatography, to quantify up to 400 low abundance peptides in human 293T cells whole cell extract. Our findings would benefit the promotion of PRM technology, especially providing a technical option for accurate quantification of low abundance proteins.