RESUMO
Objective: To increase the contents of Taxol and 10-deacetylbaccatin III in the callus of Taxus cuspidata through the application and combination of various plant growth regulators. Methods: Young stems of T. cuspidata were taken as expiants and then their effects of NAA, 2,4-D, and 6-BA on the accumulation of Taxol and 10-deacetylbaccatin III were examined with the method of orthogonal design. Results: NAA exhibited the largest effect on the yields of both total Taxol and 10-deacetylbaccatin III, and followed by 6-BA, 2,4-D. Conclusion: The optimal medium for production of total Taxol and total 10-deacetylbaccatin III is: B5+NAA 2.0 mg/L+2,4-D 0.5 mg/L+6-BA 0.2 mg/L.
RESUMO
Objective To study the HPLC fingerprints (HPLC-FPS) Taxus cuspidatea twigs and leaves (TCTL) for reflecting the internl chemical information and evaluating its internal quality of TCTL. Methods HPLC was used to analyze the alcohol extracts of TCTL. Results The fingerprint of TCTL was established. HPLC-FPS of ten batches of T. cuspidata samples collected at different time were analysed and extablished. The correlation technicl parameters of T. cuspidata were obtained, i.e. the main common peak with relative retention time and relative peak area. Conclusion The fingerprint can be used to evaluate the quality of TCTL.