Pre-germination treatments of melon seeds for the production of seedlings irrigated with biosaline water / Tratamentos pré-germinativos de sementes de melão para a produção de mudas irrigadas com água biossalina

Abstract Melon production in the Brazilian semi-arid region is subject to the use of marginal waters with high salinity. However, the use of regulators and bioactivators in seed treatment can mitigate the harmful effects of salts in irrigation water. In this context, the objective was to evaluate the effect of pre-germination treatments with plant regulators and bioactivator in melon seeds for the production of seedlings irrigated with biosaline water from fish farming effluent. For this, two trials with the Goldex and Grand Prix hybrids were carried out separately. A completely randomized design was used in a 4 × 3 factorial scheme (pre-germination treatments × water dilutions). In addition to the control, the seeds were treated with salicylic and gibberellic acids and thiamethoxam. The waters used for irrigation were local-supply water, fish farming effluent (biosaline water) and these diluted to 50%. Physiological and biochemical analyses were performed for fourteen days. Biosaline water (5.0 dS m-1) did not affect the emergence of Goldex melon seedlings, but compromised the establishment of the Grand Prix cultivar. Seed pre-treatments with salicylic and gibberellic acids attenuate the effects of water salinity and promote growth modulations, resulting in more vigorous melon seedlings.

Resumo A produção de meloeiro no semiárido brasileiro está sujeita a utilização de águas marginais com salinidade elevada. Entretanto, a utilização de reguladores e bioativadores no tratamento de sementes podem mitigar os efeitos nocivos dos sais na água de irrigação. Nesse sentido, objetivou-se avaliar o efeito de tratamentos pré-germinativos com fitorreguladores e bioativador em sementes de melão para a produção de mudas irrigadas com água biossalina de efluente de piscicultura. Para isso, dois ensaios com os híbridos Goldex e Grand Prix foram realizados separadamente. Utilizou-se delineamento inteiramente casualizado em esquema fatorial 4 × 3 (tratamentos pré-germinativos × diluições de água). Além do controle, as sementes foram tratadas com os ácidos salicílico e giberélico, e tiametoxam. As águas utilizadas para irrigação foram a de abastecimento local, efluente de piscicultura (água biossalina) e estas diluídas a 50%. Durante quatorze dias foram realizadas as análises fisiológicas e bioquímicas. A água biossalina (5,0 dS m-1) não afetou a emergência de plântulas de meloeiro Goldex, mas prejudicou o estabelecimento da cultivar Grand Prix. Os pré-tratamentos de sementes com os ácidos salicílico e giberélico atenuam os efeitos da salinidade da água e promovem modulações no crescimento, proporcionando mudas de meloeiro mais vigorosas.

Pre-germination treatments of melon seeds for the production of seedlings irrigated with biosaline water

Abstract Melon production in the Brazilian semi-arid region is subject to the use of marginal waters with high salinity. However, the use of regulators and bioactivators in seed treatment can mitigate the harmful effects of salts in irrigation water. In this context, the objective was to evaluate the effect of pre-germination treatments with plant regulators and bioactivator in melon seeds for the production of seedlings irrigated with biosaline water from fish farming effluent. For this, two trials with the Goldex and Grand Prix hybrids were carried out separately. A completely randomized design was used in a 4 × 3 factorial scheme (pre-germination treatments × water dilutions). In addition to the control, the seeds were treated with salicylic and gibberellic acids and thiamethoxam. The waters used for irrigation were local-supply water, fish farming effluent (biosaline water) and these diluted to 50%. Physiological and biochemical analyses were performed for fourteen days. Biosaline water (5.0 dS m-1) did not affect the emergence of Goldex melon seedlings, but compromised the establishment of the Grand Prix cultivar. Seed pre-treatments with salicylic and gibberellic acids attenuate the effects of water salinity and promote growth modulations, resulting in more vigorous melon seedlings.

Resumo A produção de meloeiro no semiárido brasileiro está sujeita a utilização de águas marginais com salinidade elevada. Entretanto, a utilização de reguladores e bioativadores no tratamento de sementes podem mitigar os efeitos nocivos dos sais na água de irrigação. Nesse sentido, objetivou-se avaliar o efeito de tratamentos pré-germinativos com fitorreguladores e bioativador em sementes de melão para a produção de mudas irrigadas com água biossalina de efluente de piscicultura. Para isso, dois ensaios com os híbridos Goldex e Grand Prix foram realizados separadamente. Utilizou-se delineamento inteiramente casualizado em esquema fatorial 4 × 3 (tratamentos pré-germinativos × diluições de água). Além do controle, as sementes foram tratadas com os ácidos salicílico e giberélico, e tiametoxam. As águas utilizadas para irrigação foram a de abastecimento local, efluente de piscicultura (água biossalina) e estas diluídas a 50%. Durante quatorze dias foram realizadas as análises fisiológicas e bioquímicas. A água biossalina (5,0 dS m-1) não afetou a emergência de plântulas de meloeiro Goldex, mas prejudicou o estabelecimento da cultivar Grand Prix. Os pré-tratamentos de sementes com os ácidos salicílico e giberélico atenuam os efeitos da salinidade da água e promovem modulações no crescimento, proporcionando mudas de meloeiro mais vigorosas.

Relative Toxicity of Different Insecticides to Asian Citrus Psylla (Diaphorina citri Kuwayama)

Asian citrus psyllids Diaphorina citri (Kuwayama) act as vector of the devastating citrus disease, Huanglongbing (HLB) also known as citrus greening which is widely spread in citrus growing regions. The use of insecticides for management of citrus phylla is one of the prominent and effective method to overcome their menace. However, recently very few insecticides have been found under label claim for this pest. The present investigation was conducted in order to know the toxicity of some new chemistry insecticides against D.citri. Bioassay experiments were conducted during 2022-23. The toxicity was assessed through uptake bioassay technique for systemic insecticides by using fresh citrus twigs. The results showed that, among the five tested insecticides imidacloprid 17.8 % SL was the most effective insecticide with lowest LC50 values for both nymph and adult psyllid i. e. 0.028 and 0.032, respectively. However, except spirotetramat 15.3 % OD, the LC50 values of thiamethoxam 25 % WG, abamectin 1.9 % EC, fenpropathrin 30 % EC were placed within the fiducial limit of most toxic insecticide i.e. imidacloprid 17.8% SL. They are considered as at par and equally toxic to D. citri. The relative toxicity trend against the adult psylla was imidacloprid > abamectin > thiamethoxam > fenpropathrin > spirotetramat and for the nymph, the trend was imidacloprid > thiamethoxam> abamectin > fenpropathrin > spirotetramat.

Gene Expression Endpoints Following Subchronic Thiamethoxam Exposure in Adult Male Rabbits

Aim: to investigate the sub chronic toxicity of thiamethoxam on some parameters of reproductive performance in adult male rabbits including gene expression of LDH-C4, FSH? and LH? and GnRHR. Method: sixteen adult male Chinchilla rabbits were divided into two equal groups. Animals in the first group were treated orally with TMX at dose of 250 mg/kg body weight for 90 days. The second group was served as control. Result: Obtained results showed that TMX increased the relative weight of some reproductive organs including testis and prostate. Hormonal analysis revealed that, TMX induced a significant elevation in the serum testosterone level, while the concentrations of FSH and LH hormones did not exhibit any alterations between treated and control groups. In addition, LDH-C4, FSH? and LH? and GnRHR genes were down regulated in TMX treated group. Conclusion: Administration of thiamethoxam for 90 days in male rabbits induced a noticeable adverse effect on serum testosterone level and down regulated genes related to male rabbit reproductive performance

Preliminary study on thiamethoxam degrading bacteria isolated from corn plantation

Aims@#Thiamethoxam (THIA) is a pesticide that has been widely used for its effectiveness in controlling and preventing insect pests. However, the use of THIA diffused in soil, surface and groundwater pose severe toxicity to the ecosystem. The hazardous pollution caused by the toxicity of THIA demands for remediation to ensure degradation of THIA into its safe constituent elements. Thus, the aim of this study is to isolate and identify potential THIA degraders for future bioremediation.@*Methodology and results@#Bacteria were isolated from soil sample collected at a corn plantation which utilizes THIA as a source of pesticide. Overall, two bacterial isolates were isolated from the soil sample. The bacterial isolates were screened and identified for their ability to degrade pesticide by culturing in minimal salt media (MSM) supplemented with 50 mg/L THIA. The growth of isolates was observed and analyzed through spectrophotometry analysis, standard plate count method and pH value of culture medium. As a result, isolate THIA 1 had been found to possess the ability to degrade pesticide as it showed a high rate in growth of bacteria compared to its controls. Meanwhile, isolate THIA 2 showed no degrading activities while under treatment as it showed similar rate of growth towards its control. Isolate THIA 1 was identified as Acinetobacter sp. UMTFA THIA 1.@*Conclusion, significance and impact of study@#The isolation and identification of the pesticide degrading bacteria will provide promising source of pesticide degrading enzyme that can be further developed for enzymatic pesticide biodegradation. This will pave the way forward in bioremediation where new effective degradation tools can be developed for pesticide residue which otherwise lead to serious ecological problem.

Neonicotinoid insecticides: an emerging cause of acute pesticide poisoning

Neonicotinoids are a new class of insecticides widely applied for crop protection. Information on human exposures to neonicotinoids is limited. The most common routes of exposure were ingestion (51%), dermal (44%), and ocular (11%). These insecticides act as agonists at nicotinic acetylcholine receptors, which cause insect paralysis and death the high specificity for receptors in insects was considered to possess highly selective toxicity to insects and relative sparing of mammals. However, an increasing number of cases of acute neonicotinoid poisoning have been reported in recent years. Present study report three cases presented to us with acute neonicotinoid poisoning with different manifestations including acute myocardial infarction, central nervous system (CNS) depression, and acute kidney injury, who recovered subsequently with supportive care. A detailed literature review found that respiratory, cardiovascular and certain neurological presentations are warning signs of severe neonicotinoid intoxication. Supportive treatment and decontamination are the practical methods for the management of all neonicotinoid-poisoned patients.

Effect of plant protection products on Rhynchophorus palmarum L. (Coleoptera: Curculionidae) larvae in laboratory / Efeito de defensivos agrícolas sobre larvas de Rhynchophorus palmarum L. (Coleoptera: Curculionidae) em laboratório

With the aim of developing tools for simultaneously managing Rhynchophorus palmarum and the coconut stem bleeding disease, we evaluated the effects of the insecticide thiamethoxam and the fungicide cyproconazole on R. palmarum larvae under laboratory conditions. Early-instar and intermediate-instar larvae were fed on an artificial diet containing 0.1% concentration of insecticide, fungicide or a mixture of both. Larval mortality was assessed daily during the 48 hours after the application of the treatments. Mean mortality values were subjected to analysis of variance and compared by Tukey's test (p ≤ 0.05). Thiamethoxam caused mortality of 100% of early-instar and 90% of intermediate-instar larvae. In contrast, mortality was significantly lower in the cyproconazole treatment (60% for early-instar and 0% for intermediate-instar larvae) and the control (0% mortality for both treatments). The insecticide/fungicide mixture was equally effective (100% for early-instar and 86.67% for intermediate-instar larvae) to the insecticide only treatment. Differences in mortality between early-instar and intermediate-instar larvae were significant only for the thiamethoxam and cyproconazole treatments. These results indicate that, for the doses used here, thiamethoxam is toxic to both early-instar and intermediate-instar larvae, while cyproconazole is toxic just to early-instar larvae. Moreover, cyproconazole does not increase the toxicity of thiamethoxam. Early-instar larvae are more sensitive to thiamethoxam and cyproconazole than intermediate-instar larvae. We conclude that thiamethoxam + cyproconazole mixture may be effective for managing R. palmarum and associated fungal diseases on coconut palms.(AU)

Com o objetivo de desenvolver ferramentas para o manejo simultâneo de Rhynchophorus palmarum e da resinose do coqueiro, avaliou-se o efeito do inseticida tiametoxam e do fungicida ciproconazol sobre larvas de R. palmarum em laboratório. Larvas de ínstares iniciais e intermediários foram alimentadas com dieta artificial contendo 0,1% do inseticida, do fungicida, ou da mistura de ambos. A mortalidade larval foi avaliada diariamente, durante 48 horas, e as médias foram submetidas à análise de variância e comparadas entre si pelo teste de Tukey (p ≤ 0,05). O tiametoxam causou mortalidade de 100% às larvas de ínstares iniciais e de 90% às de intermediários. Em contraste, a mortalidade foi significativamente menor no tratamento ciproconazol (60% para larvas de ínstares iniciais e 0% para as de intermediários) e no controle (0% de mortalidade para ambos). A mistura inseticida/fungicida foi igualmente eficiente (100% para larvas de ínstares iniciais e 86,67% para as de intermediários) ao inseticida sozinho. Diferenças na mortalidade entre as larvas de ínstares iniciais e intermediários foram significativas somente para os tratamentos tiametoxam e ciproconazol. Tais resultados indicam que, nas doses avaliadas, o tiametoxam é tóxico às larvas de ínstares iniciais e intermediários, enquanto o ciproconazol é tóxico somente às de ínstares iniciais. Além disso, o ciproconazol não aumenta a toxicidade do tiametoxam. Larvas de ínstares iniciais são mais sensíveis a tiametoxam e ciproconazol que as de ínstares intermediários. Conclui-se que a mistura tiametoxam + ciproconazol pode ser eficiente no manejo de R. palmarum e de doenças fúngicas associadas a essa praga em coqueiros.(AU)

Toxicidade do inseticida Tiametoxam para o Pacamã (Lophisiolurus alexandri) / Toxicity of Thiamethoxam insecticide for Pacamã (Lophisiolurus alexandri)

Este estudo avaliou a toxicidade aguda e crônica de um inseticida a base de tiametoxam para peixes da espécie Pacamã (Lophisiolurus alexandri), avaliando a CL50%, além de alterações histopatológicas hepáticas em animais expostos por 96 horas e 15 dias ao inseticida. Para tanto foram utilizados 120 alevinos submetidos a cinco diferentes concentrações do inseticida (30, 60, 120, 240 e 480mg/L) por 15 dias, com dois tempos amostrais, 96 horas e 15 dias. Não houve mortalidade significativa durante todo o período experimental, no entanto os animais apresentaram alterações como vacuolização citoplasmática, congestão e necrose. A CL50% foi determinada como superior a 100mg/L, considerada praticamente não tóxico. A necrose foi a alteração melhor evidenciada nos animais expostos, com o aumento da ocorrência nos animais do teste de toxicidade crônica.(AU)

This research evaluated acute and chronic toxicity of a thiamethoxam insecticide for Pacamã (Lophisiolurus alexandri), assessing the LC50 and liver histopathological changes in animals exposed for 96 hours and 15 days. Therefore, were used 120 fingerlings subjected to five different concentrations (30, 60, 120, 240 and 480mg/L) for 15 days with two sampling times, 96 hours and 15 days. There was no significant mortality during the experimental period, however the animals showed changes as vacuolation, congestion and necrosis. The LC50 was determined as greater than 100mg/L, considered practically non-toxic. Necrosis was the more significant change in exposed animals, with increasing occurrence in chronic toxicity test.(AU)

Adulticidal & larvicidal efficacy of three neonicotinoids against insecticide susceptible & resistant mosquito strains.

Background & objectives: Due to ever growing insecticide resistance in mosquitoes to commonly used insecticides in many parts of the globe, there is always a need for introduction of new insecticides for the control of resistant vector mosquitoes. In this study, larvicidal and adulticidal efficacies of three neonicotinoids (imidacloprid, thiacloprid and thiamethoxam) were tested against resistant and susceptible populations of Anopheles stephensi Liston 1901, Aedes (Stegomyia) aegypti Linnaeus, and Culex quinquefasciatus Say (Diptera: Culicidae). Methods: Laboratory-reared mosquito species were used. Insecticide susceptibility tests were done using standard WHO procedures and using diagnostic dosages of insecticide test papers and larvicides. Adulticidal efficacy of candidate insecticides was assessed using topical application method and larval bioassays were conducted using standard WHO procedure. Results: The results of topical application on 3-5 day old female mosquitoes indicated that resistant strain of An. stephensi registered lower LC50 values than the susceptible strain. Among the three insecticides tested, thiacloprid was found more effective than the other two insecticides. Culex quinquefasciatus registered lowest LC50 for imidacloprid than the other two mosquito species tested. In larval bioassays, the LC50 values registered for imidacloprid were in the order of Cx. quinquefasciatus <An. stephensi (SS) <An. stephensi (RR) <Ae. aegypti. In case of thiacloprid, the order of efficacy (LC50) was Cx. quinquefasciatus <An. stephensi (SS) <An. stephensi (RR), whereas in case of thiamethoxam, the larvicidal efficacy was in the order of An. stephensi (RR) <An. stephensi (SS) <Cx. quinquefasciatus. Interpretation & conclusions: The present study indicated that insecticide resistant strains of mosquito species tested showed more susceptibility to the three neonicotinoids tested, and the possibility of using neonicotinoids for the control of resistant mosquitoes should be explored.

Preliminary Study on Influence of Component Content Indexes in Honeysuckle by Thiamethoxam / 世界科学技术-中医药现代化

This study was aimed to investigate the influence on component content indexes,which included chlorogenic acid and luteolin of honeysuckle by the absorption of chemical insecticide thiamethoxam.High-performance liquid chromatography (HPLC) method was used in the analysis of content determination of component content indexes of honeysuckle samples by different concentrations of thiamethoxam.SPSS was used in the statistical analysis.The results showed that compared to samples from the blank control group,the content of chlorogenic acid had the increasing tendency in the honeysuckle groups with different concentrations of thiamethoxam.However,there was no significant difference among different experiment groups.There was no significant difference on luteolin among honeysuckle groups with different concentrations of thiamethoxam and the blank control group.It was concluded that at the recommended dose and high-powered dose of thiamethoxam,there was some influence on the content of chlorogenic acid.However,there was no significant effect on the content of luteoloside.And the quality scores of both contents were within the range defined in the Chinese Pharmacopoeia(2010 edition).

Tratamento de sementes com fungicidas e inseticidas como redutores dos efeitos do estresse hídrico em plantas de soja / Seed treatment with fungicides and insecticides reducing the hydric stress on soybean plants

O objetivo do trabalho foi avaliar o efeito de quatro tratamentos de sementes: água (T1), carbendazim + thiram (T2), fipronil + tiofanato metílico + piraclostrobina (T3) e abamectina + thiamethoxam + fludioxonil + mefenoxan + thiabendazole (T4) sobre parâmetros de crescimento de plantas de soja submetidas a dois regimes hídricos (presença e ausência de estresse hídrico) estabelecidos logo após a emergência das plântulas. O experimento foi conduzido em casa de vegetação e campo. Na casa de vegetação, nos tratamentos sem estresse hídrico, foram feitas quatro irrigações por dia e, nos tratamentos com estresse hídrico, foi realizada uma irrigação a cada três dias. Neste ensaio, foi avaliada a estatura de plantas, comprimento radicular, volume radicular, massa seca da parte aérea, massa seca radicular e área foliar. No ensaio de campo, o estresse hídrico foi estabelecido pela construção de túneis baixos de polietileno que impediram o fornecimento de água pela irrigação e chuva. No campo, foram avaliados estatura de planta, teor relativo de clorofila, emergência, ataque de lagarta-elasmo e rendimento de grãos. Nos dois ambientes, as plantas foram mantidas sob estresse até 28 dias após a emergência. Em casa de vegetação, todos os parâmetros foram influenciados pelo tratamento de sementes na presença e ausência de estresse hídrico. No campo, o tratamento com fipronil + tiofanato metílico + piraclostrobina (T3) e abamectina + thiamethoxam + fludioxonil + mefenoxan + thiabendazole (T4) proporcionaram maior estatura de planta e teor relativo de clorofila em ambos os regimes hídricos e maior emergência e rendimento de grãos em plantas sem e com restrição hídrica, respectivamente. Conclui-se que o tratamento de sementes com esses produtos promove alterações benéficas nas plantas, aumentando sua tolerância ao estresse hídrico, com efeito positivo sobre o rendimento de grãos da soja.

The objective of this research was evaluate four seed treatments: water (T1), carbendazim + thiram (T2), fipronil + thiophanate methyl + pyraclostrobin (T3) and abamectin + thiamethoxam + fludioxonil + mefenoxan + thiabendazole (T4) on growth parameters of soybean plants with or without low water availability. The experiments were conducted under field conditions and greenhouse. In greenhouse, in treatments without low water availability, it was made four irrigations by day and in treatments with low water availability, it was made one irrigation each three days. In this trial, it was evaluated the plant height, root length, root volume, dry shoot weight, dry root weight and foliar area. In the field trial, low water availability was established by construction of low tunnels of polyethylene which prevented the water supply by irrigation and rain. In the field, it was also evaluated the plant height, the relative chlorophyll content, emergence, lesser cornstalk borer attack and grain yield. In the both environment, the plants were kept on stress until 28th days after emergence. In greenhouse, all the parameters were influenced by seed treatments with or without low water availability. In field, the treatment with fipronil + thiophanate methyl + pyraclostrobin (T3) and abamectin + thiamethoxam + fludioxonil + mefenoxan + thiabendazole (T4) promoted higher plant height and relative chlorophyll content in both hydric schemes, higher emergence and grain yield in plants without and with lower water availability, respectively. It was concluded that seed treatment with these products promote benefit changes in plant, increasing its tolerance to hydric stress, with positive effect on soybean grain yield.

Laboratory and field evaluation of Metarhizium anisopliae var. anisopliae for controlling subterranean termites

The efficacy of the Metarhizium anisopliae strain ARSEF 6911 was determined in the laboratory and field against two sugarcane pests, Microtermes obesi Holmgren and Odontotermes obesus Rambur (Termitidae: Isoptera). The susceptibility of both termite species to different conidial suspensions (1 × 10(10), 1 × 10(8), 1 × 10(6) and 1 × 10(4) conidia/ml) was determined in laboratory. All conidial suspensions were able to induce mortality. Termite mortality caused by the fungal suspensions was dose dependent. There were no significant differences in the LT50 values between species. Field evaluation of M. anisopliae alone or in combination with diesel oil and thiamethoxam was carried out in two growing seasons (autumn 2005 and spring 2006) at two sites located in Punjab, Pakistan. Dipping the sugarcane setts in these suspensions was tried to determine their effects on germination and percentage of bud damage to sugarcane setts. All treatments significantly reduced termite infestation compared to the untreated control. The combined treatment of M. anisopliae and diesel oil significantly reduced insect damage by attaining higher germination > 55 percent and lower bud damage < 5.50 percent at both sites in both seasons. The results suggest that the application of M. anisopliae and diesel oil in combination might be a useful treatment option for the management of termites in sugarcane.

Compatibilidade de inseticidas e acaricidas com o percevejo predador Podisus nigrispinus (Dallas) (Heteroptera: Pentatomidae) em algodoeiro / Compatibility of insecticides and acaricides to the predatory stinkbug Podisus nigrispinus (Dallas) (Heteroptera: Pentatomidae) on cotton

The objectives of this work were to study the insecticides espinosad, lambdacyhalothrin and monocrotophos on the control of Alabama argillacea (Hübner), the compatibility of these insecticides with the predatory stinkbug Podisus nigrispinus (Dallas), and the effects of the insecticides and acaricides methiocarb, pymetrozine, thiamethoxam, abamectin, diafenthiuron, dicofol and propargite on the predatory stinkbug behavior preying upon A. argillacea under field conditions. More than 90 percent of cotton leafworm control was obtained at the day 0 of application with all insecticides. Control of A. argillacea by lambdacyhalothrin (20 g a.i./ha) and monocrotophos (240 and 320 g a.i./ha) was observed up to day 3 after application; while spinosad (30 and 60 g a.i./ha) prolonged control up to day 5. Monocrotophos and lambdacyhalothrin were significantly more toxic to P. nigrispinus compared to spinosad. Toxicity of monocrotophos and lambdacyhalothrin to P. nigrispinus was evident up to day 3 after application. Survival of P. nigrispinus was affected by methiocarb, thiamethoxam, diafenthiuron, abamectin and sulfur one day after application and only under thiamethoxam treatment after three days. Pymetrozine, dicofol and propargite did not affect P. nigrispinus survival compared to untreated plants. Podisus nigrispinus at the day of application did not prey on A. argillacea under methiocarb treatment, and the predation rate was affected under thiamethoxam, abamectin, diafenthiuron and sulfur treatments at the day of application and after three days. The insecticides pymetrozine and spinosad, and the acaricides dicofol and propargite were compatible to P. nigrispinus and should be considered within a cotton pest management program.

Os objetivos deste trabalho foram estudar a ação dos inseticidas espinosade, lambdacialotrina e monocrotofós no controle do curuquerê-do-algodoeiro Alabama argillacea (Hübner), sua compatibilidade com o percevejo predador Podisus nigrispinus (Dallas), e os efeitos dos inseticidas e acaricidas metiocarbe, pimetrozine, tiametoxam, abamectim, diafentiurom, dicofol e propargite na predação de A. argillacea por P. nigrispinus no campo. Inseticidas e concentrações utilizadas proporcionaram controle de A. argillacea acima de 90 por cento no dia da aplicação. O controle de A. argillacea por lambdacialotrina (20 g i.a./ha) e monocrotofós (240 e 320 g i.a./ha) foi observado até três dias após aplicação, enquanto espinosade (30 e 60 g i.a./ha) prolongou o controle acima de 90 por cento até cinco dias. Monocrotofós e lambdacialotrina foram significativamente mais tóxicos para P. nigrispinus comparado a espinosade. O efeito residual de monocrotofós e lambdacialotrina para P. nigrispinus foi evidente até três dias após a aplicação. A sobrevivência de P. nigrispinus foi afetada por metiocarbe, tiametoxam, diafentiurom, abamectim e enxofre após um dia, e apenas por tiametoxam após três dias da aplicação. Pimetrozine, dicofol e propargite não afetaram a sobrevivência de P. nigrispinus. A predação de lagartas de A. argillacea por P. nigrispinus não foi observada no dia da aplicação em plantas tratadas com metiocarbe e afetada por tiametoxam, abamectim, diafentiurom e enxofre, mesmo três dias após a aplicação. Os inseticidas pimetrozine e espinosade, bem como os acaricidas dicofol e propargite mostraram-se compatíveis com P. nigrispinus, e devem ser priorizados em programas de manejo integrado de pragas do algodoeiro.