Study on the relationship between organic amines derived from putrefaction of isolated human hepatic tissues and the time of death under different environmental temperatures / 中国法医学杂志

Objective To search some references for estimating the time of death in some murder cases by observation the dynamic changes of organic amines derived from isolated human hepatic tissues. Method The contents of histamines, putrescines, cadaverines and undecomposed amino acids from the isolated human hepatic ussues were assayed dynamically by the method of high performance liquid chromatography (HPLC). Results The contents of the organic amines produced by the putrefaction under 8℃,15℃ , 23℃ and 32℃ were increased along with the time elapsed after death under four environmental temperatures before attaining to their peak value, While the contents of the undecomposed amino acids were decreased gradually after death. Conclusion The amount of organic amines produced by putrefaction of the isolated human hepatic tissues were directly related to the time of death. So do the environmental temperature.

THE POSTMORTEM ENZYME ACTIVITY CHANGES IN MICE'S HEPATIC CELLS / 中国法医学杂志

Timing of death is one of the important problems in forensic pathologic practice.Using histochemical methods,the authors observed the enzyme activity changes ofPPr,CCo,ACP,ANAE,LDH,SDH,G6PD,NADHD,G-6-Pase and ATPase inmice liver cells at 0,6,12,24,48,72 hours postmortem(HPM) at the environmen-tal temperature 4℃ and 20℃.The results showed that at 20℃,PPr activity dec-reased markedly at 6 HPM,LDH and ATPase activity decreased at 48 HPM anddisappeared at 72 HPM.The another seven enzymes still showed good enzyme acti-vity at 72 HPM.The authors supposed that the results are significant for timingof postmortem duration.