RESUMO
OBJECTIVE:To observe the effects of total organic acid of Thladiantha dubia fruit(TOATF)on coagulation time and hemorheology of rats with cold coagulation and blood stasis. METHODS:60 rats were randomly divided in normal control group (water),model control group (water),aspirin group (positive drug,50 mg/kg) and TOATF low-dose,medium-dose and high-dose groups(50,100,200 mg/kg)with 10 rats in each group. Except for normal control group,cold coagulation and blood stasis model was induced by 4 ℃ water bath and subcutaneous injection of adrenaline hydrochloride,and then given correspon-dence medicine intragastrically,once a day,for consecutive 14 d. 24 h after last administration,blood samples were collected from aorta abdominalis. The coagulating time(CT),erythrocrit(HCT),plasma viscosity(PV),prothrombin time(PT),thrombin time (TT),activated partial thromboplastin time (APTT),platelet aggregation rate (PAR) and whole blood low-shear,middle-shear and high-shear viscosity were measured and blood sedimentation equation K value was calculated. RESULTS:Compared with nor-mal control group,CT,PT,TT and APTT of model control group were shortened,and HCT,PV,blood sedimentation equation K value,PAR and whole blood low-shear,middle-shear and high-shear viscosity increased(P<0.05 or P<0.01). Compared with model control group,CT,PT and APTT prolonged in aspirin group and TOATF medium-dose and high-dose groups,and PAR de-creased;PT of treatment groups prolonged,while HCT,PV,blood sedimentation equation K value and whole blood low-shear and middle-shear viscosity decreased(P<0.05 or P<0.01). CONCLUSIONS:TOATF has obvious improvement effects on anticoagula-tion and hemorheology in rats with cold coagulation and blood stasis.
RESUMO
Objective: To study the active components with anti-bacterial effect in Isatidis Radix. Methods: Using cylinder-plate method with bacteriostatic coefficient as the index, the anti-Escherichia coli AG100A effects of five extracts (total extract, tatal organic acid extract, total alkaloids extract, tatal nudeoside extract, and total anthraquinone extract) from Isatidis Radix were studied. After the determination of the most active extract, HPLC fingerprints on 10 batches of Isatidis Radix from different habitats were established. The correlations between areas of common peaks and bacteriostatic coefficients were analyzed by principal component analysis (PCA). Results: The total organic acid extract showed the best anti-bacterial effect, with 21 common peaks in HPLC fingerprint. The results of the PCA demonstrated that the common peaks 2, 10, 16, 18, 20, and 21 were connected closely with the antibacterial activity, among which the common peaks 10 and 18 were proved to be salicylic acid and benzoic acid, respectively. Conclusion: The total organic acid extract from Isatidis Radix shows the significant antibacterial effect, in which salicylic acid and benzoic acid are the active chemical constituents with the antibacterial activity.