Protective effect of trans-nerolidol on vascular endothelial cell injury induced by lipopolysaccharides / Efectos protectores del trans-nerolidol en lesiones inducidas por lipopolisacáridos en células endoteliales vasculares

This article aimed to discuss the protection of trans - nerolidol on vascular endothelial cells (ECs) injured by lipopolysac charides. ECs were divided into four groups: normal, model, low and high dose trans - nerolidol treatment groups. The cell survival rate and the contents of NO in the cell culture supernatant were determined. The protein expression and transcript level of pe roxisome proliferator - activated receptor - γ (PPARγ), endothelial nitric oxide synthase (eNOS), and inducible nitric oxide synthase (iNOS) were determined by western blotting and RT - PCR respectively. Compared with the normal group, cell livability, protein e xpression and mRNA transcript level of PPARγ and eNOS decreased, NO contents, protein expression and mRNA transcript tlevel of iNOS increased in model group significantly. Compared with model group, all the changes recovered in different degree in treatmen t groups. Hence, it was concluded that trans - nerolidol can alleviate the ECs injuryby the regulation of iNOS/eNOS through activating PPARγ in a dose - dependent manner

Este artículo tiene como objetivo discutir la protección del trans - nerolidol en las células endoteliales vasculares (CE) dañadas por lipopolisacáridos. Las CE se di vidieron en cuatro grupos: normal, modelo, grupos de tratamiento con trans - nerolidol de baja y alta dosis. Se determinó la tasa de supervivencia de las células y los contenidos de óxido nítrico (NO) en el sobrenadante del cultivo celular. La expresión de p roteínas y el nivel de transcripción del receptor activado por proliferadores de peroxisomas - γ (PPARγ), el óxido nítrico sint et asa endotelial (eNOS) y el óxido nítrico sint et asa inducible (iNOS) se determinaron mediante western blot y RT - PCR, respectivamen te. En comparación con el grupo normal, la viabilidad celular, la expresión de proteínas y el nivel de transcripción de PPARγ y eNOS disminuyeron, los contenidos de NO, la expresión de proteínas y el nivel de transcripción de iNOS aumentaron significativam ente en el grupo modelo. En comparación con el grupo modelo, todos los cambios se recuperaron en diferentes grados en los grupos de tratamiento. Por lo tanto, se concluyó que el trans - nerolidol puede aliviar el daño en las CE regulando iNOS/eNOS a través d e la activación de PPARγ de manera dependiente de la dosis.

Valoración de la densidad mineral ósea en personas transgénero posicionamiento de la Sociedad Argentina de Osteoporosis / Evaluation of bone health in transgender people. Position of the Argentine Osteoporosis Society

Resumen La Sociedad Argentina de Osteoporosis convocó a especialistas reconocidos en la atención de personas transgénero para la elaboración del primer posiciona miento local sobre la evaluación de la salud ósea en esta población. La ley 26.743 de "Identidad de género" reco noce todas las identidades y garantiza su atención de manera gratuita en el sistema de salud. El impacto de los diferentes tratamientos de afirmación de género sobre la masa ósea ha sido tópico de debate internacional. Hasta la fecha la evidencia sigue siendo limitada y diferentes sociedades han emitido sugerencias y recomendaciones. Por tal motivo, creemos relevante mencionar nuestra experiencia plasmando mediante este documento una serie de sugerencias para ser utilizadas en la atención médica.

Abstract The Argentine Osteoporosis Society convened renowned specialists in the care of transgender people to prepare the first local position on the evaluation of bone health in this population. Law 26.743 on "Gender Identity" recognize all identities and guarantees free care throughout the health system. The impact of different gender affirmation treatments on bone mass has been topic of international debate. To date the evidence remains limited and different societies have issued suggestions and recommendations. For this reason, we believe it is relevant to mention our experience, capturing through this document a series of suggestions to be used in medical care.

"Aquende a neca e aperte os seios": inteligibilidade, trabalho e saúde nas experiências de pessoas trans universitárias / "Tuck the girldick and bind the breasts:" intelligibility, work, and health in the experiences of university trans people

Resumo As pessoas trans estão expostas a diferentes situações de vulnerabilidade que se entrecruzam, causando impactos negativos no processo saúde-doença-cuidado. Para melhor compreender essa questão, buscamos conhecer as experiências de sete estudantes trans de uma universidade pública de Minas Gerais, a partir de entrevistas norteadas por roteiro semiestruturado. As falas foram analisadas pela perspectiva da análise de conteúdo, e as categorias finais foram tensionadas com estudos das ciências sociais e da saúde coletiva. De forma geral, as narrativas se diferenciaram daquelas que compõem a maioria das pesquisas sobre o tema no Brasil, sobretudo em relação à manutenção de vínculos familiares e ao acesso à universidade pública. Nesse sentido, formulamos a hipótese de que a interação desses elementos com outros marcadores de diferenças pode contribuir positivamente para o estabelecimento de alianças e estratégias, a fim de lidar com o regime de inteligibilidade das identidades de gênero e com as dificuldades de acesso à educação, ao trabalho e à saúde. Compreender essa dinâmica e suas limitações, em uma perspectiva em que o individual se enlaça ao coletivo, possibilitou evidenciar o funcionamento da (hétero)normatividade nos processos de segregação de pessoas trans, assim como refletir sobre ações políticas que possam transformar essa realidade social efetivamente.

Abstract Transgender people are exposed to different situations of vulnerability that intersect, having negative impacts on the health-illness-care process. To better understand this issue, we sought to know the experiences of seven transgender undergraduates at a public university in Minas Gerais, from semi-structured interviews. The narratives were analyzed on the perspective of content analysis, and the final categories juxtaposed with studies of social sciences and public health. In general, the narratives are different from those which account for most of the research on the theme in Brazil, especially regarding maintaining family bonds and the access to public universities. Hence, we formulated the hypothesis that the interaction between these elements and other markers of differences can positively contribute to establishing alliances and strategies to deal with the regime of gender identity intelligibility and the constraints of access to education, work, and health. Understanding these dynamics and their limitations, under the perspective in which the individual and the collective are intertwined, enabled us to highlight the role of (hetero)normativity in the segregation processes of transgender people, as well as reflecting on the political actions that might effectively transform this social reality.

Corpos transmasculinos negros em intersecções estéticas: entrevista com Leonardo Peçanha / Black transmasculine bodies in aesthetic intersections: an interview with Leonardo Peçanha

Resumo Este artigo de opinião consiste em uma entrevista realizada com Leonardo Peçanha, um homem negro trans, ativista no campo dos direitos humanos e dos direitos das pessoas trans e pesquisador do campo da Saúde Coletiva. Na conversa que se segue Leonardo Peçanha, de forma interseccional, reflete sobre o lugar da beleza, modificações corporais e atividade física no processo de transição de gênero de sujeitos homens trans e transmasculinos, como também toca no tema das (im)possibilidades das pessoas trans no campo dos esportes de alto rendimento e nos conta sobre o concurso de beleza Mister Trans Brasil.

Abstract This opinion article consists of an interview with Leonardo Peçanha, a Black trans man, a human rights and trans people rights activist and a researcher in Collective Health. In this interview, he reflects intersectionally on the place of beauty, body changes, and physical activity in the gender transition process of trans and transmasculine men. He discusses the (im)possibilities of trans people in high-performance sports and tells us about the Mister Trans Brazil beauty pageant contest.

Effects of metabolites of eicosapentaenoic acid on promoting transdifferentiation of pancreatic OL cells into pancreatic β cells / 中国药理学通报

Aim To investigate the role of metabolites of eicosapentaenoic acid (EPA) in promoting the transdifferentiation of pancreatic α cells to β cells. Methods Male C57BL/6J mice were injected intraperitoneally with 60 mg/kg streptozocin (STZ) for five consecutive days to establish a type 1 diabetes (T1DM) mouse model. After two weeks, they were randomly divided into model groups and 97% EPA diet intervention group, 75% fish oil (50% EPA +25% DHA) diet intervention group, and random blood glucose was detected every week; after the model expired, the regeneration of pancreatic β cells in mouse pancreas was observed by immunofluorescence staining. The islets of mice (obtained by crossing GCG

Functions of HIV-1 Tat and its association with diseases / 中国生物制品学杂志

@#Acquired immune deficiency syndrome,or AIDS,has been a major infectious disease that troubles the public health in a global scale. Human immunodeficiency virus type 1(HIV-1)is the causative reagent responsible for AIDS development. Even though the highly active anti-retroviral therapy(HAART,or the cocktail therapy)that has been widely applied could effectively suppress the infection and replication of HIV-1,the infected people suffer from other related diseases,such as the HIV-associated neurocognitive disorder(HAND). This paper mainly focused on the function of an important regulatory protein of HIV-1,trans-activator of transcription(Tat),and its correlation with HIV-1 replication and HAND development,so as to clarify the importance of developing anti-AIDS drugs targeting Tat protein

Impacto psicossocial da pandemia de COVID-19 na saúde mental de pessoas transexuais e travestis: revisão integrativa / Psychosocial impact of COVID-19 pandemic on mental health of transgender and transvestite people: integrative review / Impacto psicosocial de COVID-19 en la salud mental de las personas transgénero y travestis: revisión integrativa

Este estudo objetivou identificar, analisar e integrar resultados de estudos que examinaram o impacto da pandemia de COVID-19 na saúde mental de pessoas transexuais e travestis. Foi realizada uma revisão integrativa da literatura, em conformidade com as diretrizes PRISMA. Conduziu-se uma busca sistemática dos estudos primários publicados em três bases de dados: PubMed/MEDLINE, LILACS e PsycINFO. Foram incluídos oito estudos que atenderam aos critérios de inclusão. A análise temática do material gerou seis categorias temáticas. Os estudos analisados apontaram que a situação de vulnerabilidade social ao qual as pessoas trans encontram-se submetidas no cotidiano foi ampliada durante a pandemia. A necessidade do distanciamento físico resultou no aumento de sintomas de ansiedade e depressão, agravamento das condições econômicas, dificuldades para acessar procedimentos e medicamentos que compõem o processo de afirmação de gênero, além de exacerbar conflitos familiares e manifestações de transfobia. Há necessidade de implementar políticas públicas e uma rede de proteção social para diminuir a vulnerabilidade e sofrimento psicossocial de pessoas trans. (AU)

This study aimed to identify, analyze, and integrate findings from studies that examined the impact of the COVID-19 pandemic on the mental health of transgender and transvestite individuals. An integrative literature review was conducted according to PRISMA guidelines. A systematic search of primary studies was conducted across three databases: PubMed/MEDLINE, LILACS, and PsycINFO. Eight studies that met the inclusion criteria were included. Thematic analysis of the material generated six thematic categories. The studies analyzed pointed out that the situation of social vulnerability faced by trans people in their daily lives intensified during the pandemic. The need for physical distancing led to increased symptoms of anxiety and depression, worsening economic conditions, and difficulties in accessing procedures and medications that make up the gender affirmation process, in addition to exacerbating family conflicts and manifestations of transphobia. Therefore, there is a need to implement public policies and a social protection network to reduce the vulnerability and psychosocial suffering of the transgender and transvestite community. (AU)

El objetivo de este estudio es identificar, analizar e integrar los resultados de los estudios que examinaban el impacto de la pandemia de COVID-19 en la salud mental de las personas transexuales y travestis. Se realizó una revisión integrativa de acuerdo con las directrices PRISMA en tres bases de datos: PubMed/MEDLINE, LILACS y PsycINFO. Se incluyeron ocho estudios que cumplían con los criterios de inclusión. El análisis temático del material generó seis categorías temáticas. Los estudios analizados señalaron que la situación de vulnerabilidad social a la que están sometidas las personas trans en la vida cotidiana se amplió durante la pandemia. La necesidad de distanciamiento físico se tradujo en el aumento de los síntomas de ansiedad y depresión, el agravamiento de las condiciones económicas, las dificultades para acceder a los procedimientos y medicamentos que componen el proceso de afirmación del género, además de exacerbar los conflictos familiares y las manifestaciones de transfobia. Es necesario aplicar políticas públicas y una red de protección social para reducir la vulnerabilidad y el sufrimiento psicosocial de las personas transgénero. (AU)

A systematic review and meta-analysis of trans-diagnostic third-wave online psychological interventions / Revisión sistemática y metaanálisis de intervenciones transdiagnósticas en línea de tercera generación

Abstract Existing meta-analyses on the effect of online psychological interventions (OPIs) have found small to medium effect sizes for the treatment of anxiety and depression. On the other hand, third-generation trans-diagnostic OPIs are very rare and, due to the large variability among disorders, symptoms or target populations, it is difficult to assess their overall effect. Other systematic reviews and meta-analyses have overly broad inclusion criteria that make the understanding of the findings more difficult. The current study aims to analyze the empirical evidence for third-wave trans-diagnostic OPIs designed to decrease symptoms and promote psychological flexibility, including studies that compare a OPI to some control condition (e. g., waiting list, treatment as usual or other that should not have any effect) and include a general symptomatology scale as dependent variable. A search without filters or timeframe was performed on Scopus and 1 408 articles were found, among which 21 were reviewed in depth and 6 were included for meta-analysis. Risk of bias was assessed by a quality and heterogeneity assessment. Separate meta-analyses were performed for general distress and psychological flexibility at post-treatment and last follow-up. Risk of bias analysis suggest low risk of threats to validity and attribute heterogeneity to between-study attrition rates. Additionally, meta-regression models for duration, attrition rate, and mean age are proposed for each time point. The results show significantly large effect sizes for both variables at both time points. According to the meta-regression models attrition rates are a mediating variable for the effect on general distress both at completion and at the last follow-up. On the other hand, duration, age and attrition rate are all mediating variables for the effect on psychological flexibility at the end of treatment. The findings suggest that the high attrition rates observed on tele-psychology need to be mitigated; if this is not possible, intention-to-treat approaches should be adopted for data analysis.

Resumen Los metaanálisis existentes sobre el efecto de las intervenciones psicológicas en línea (OPIs, por sus siglas en inglés) han encontrado tamaños de efecto pequeños o medianos para el tratamiento de la ansiedad y la depresión. Por otra parte, las OPIs transdiagnósticas de tercera generación son muy escasas y, debido a la gran variabilidad entre los trastornos, los síntomas o las poblaciones objetivo, es difícil evaluar su efecto global. Otras revisiones sistemáticas y metaanálisis tienen criterios de inclusión demasiado amplios que dificultan la comprensión de los hallazgos. El presente estudio tiene como objetivo analizar la evidencia empírica actual para las OPIs transdiagnósticas de tercera generación diseñadas para disminuir los síntomas y promover la flexibilidad psicológica, incluyendo estudios que comparen una OPI con alguna condición de control (por ejemplo lista de espera, tratamiento habitual u otro que no debería tener efecto) y que incluyan una escala de sintomatología general como variable dependiente. Se excluyeron estudios basados en otras terapias y/o diseñados para prevenir o tratar una población, un trastorno o un conjunto de síntomas específicos. También se excluyeron los protocolos de estudio, los diseños pretest-postest y otros en los que era imposible calcular el tamaño del efecto. Se realizó una búsqueda sin filtros ni marco temporal en Scopus y se encontraron 1 408 artículos entre los cuales 21 fueron revisados en profundidad y 6 fueron incluidos en el presente estudio. El riesgo de sesgo se evaluó mediante una evaluación de calidad y heterogeneidad; no fue posible realizar análisis de sesgo de publicación. Se realizaron metaanálisis separados para el malestar general y la flexibilidad psicológica en postratamiento y último seguimiento. El análisis del riesgo de sesgo sugiere un bajo riesgo de amenazas a la validez y atribuye la heterogeneidad principalmente a las tasas de deserción entre los estudios. Además, se proponen modelos de metarregresión para la duración, la tasa de deserción y la edad promedio en cada punto temporal. Los resultados muestran tamaños de efecto significativamente grandes para ambas variables en ambos puntos temporales y se evalúa su heterogeneidad, que se atribuye en gran medida a las tasas de deserción de los estudios incluidos. Según los modelos de metarregresión, las tasas de deserción son una variable mediadora del efecto sobre el malestar general tanto en el momento de la finalización como en el último seguimiento. Por otra parte, la duración, la edad y la tasa de abandono son variables mediadoras del efecto sobre la flexibilidad psicológica al final del tratamiento. Los resultados sugieren que es necesario mitigar las altas tasas de deserción observadas en la telepsicología y, cuando no sea posible, adoptar enfoques de intención de tratar para el análisis de los datos.

Acute promyelocytic leukemia in childhood and adolescence: treatment results of a modified AIDA protocol at a Brazilian center

ABSTRACT Introduction: Acute promyelocytic leukemia currently presents an excellent chance of cure with protocols based on all-trans-retinoic acid (ATRA) and anthracycline or only differentiation agents. However, high early mortality rates continue to be reported Methods: Between 2000 and 2018, patients were enrolled and retrospectively analyzed by medical records. A modified AIDA protocol, with a 1-year shortening of the treatment duration, reduction in the number of drugs and a strategy to reduce early mortality by the postponement of the initiation of anthracyclines were employed. Overall and event-free survival rates and toxicity were analyzed Results: Thirty-two patients were enrolled, of whom 56% were female, with a median age of 12 years and 34% belonged to the high-risk group. Two patients had the hypogranular variant and three had another cytogenetic alteration, in addition to the t(15;17). The median start of the first anthracycline dose was 7 days. There were two early deaths (6%) due to central nervous system (CNS) bleeding. All patients achieved molecular remission after the consolidation phase. Two children relapsed and were rescued by arsenic trioxide and hematopoietic stem cell transplantation. The presence of disseminated intravascular coagulation (DIC) at diagnosis (p = 0.03) was the only factor with survival impact. The five-year event-free survival (EFS) was 84% and 5-year overall survival (OS) was 90% Conclusion: The survival results were comparable to those found in the AIDA protocol, with a low rate of early mortality in relation to the Brazilian reality.

Trans-palmitoleic acid does not modify the inflammasome expression in a rodent model of diet-induced obesity / El ácido trans-palmitoléico no modifica la expresión del inflamasoma en un modelo murino de obesidad inducida por dieta

Abstract Background: Metabolic disorders such as obesity and type 2 diabetes (T2D) coincide with an increased expression of pro-inflammatory factors. The NLRP3 inflammasome is a complex that activates the pro-inflammatory cytokine IL-1β. (NOD-like receptor protein 3). Some nutrients, such as fatty acids, influence inflammatory processes. For example, in clinical studies, higher trans-palmitoyl acid (TP) concentrations coincide with lower adiposity and lower risk of developing T2D. This study aims to evaluate the effect of TP on NLRP3 expression in a rodent model of diet-induced obesity (DIO). Methods: C57BL/6J mice were fed ad libitum with a control or a high-fat diet (HFD), added with or without TP (3 g/kg diet), for 11 weeks. IL-1β was quantified in serum, and NLRP3-related gene expression was explored in epididymal adipose tissue. Results: Despite increased weight gain in both high-fat groups, the high-fat TP group gained less weight than the high-fat group. In addition, NLRP3 and caspase-1 expression was higher in the HFD groups, but no differences were observed between the HFD and the HFD TP groups. Serum IL-1β levels were not different among groups. Conclusions: Diet supplementation with TP prevents weight gain and has a neutral influence over NLRP3 expression and IL-1β concentration in a DIO mice model.

Resumen Introducción: Las alteraciones metabólicas como la obesidad y diabetes tipo 2 (DT2) coinciden con la expresión aumentada de factores proinflamatorios. Un complejo que induce la activación de la citocina proinflamatoria IL-1β es el inflamasoma NLRP3 (NOD-like receptor protein 3). Algunos nutrimentos, como los ácidos grasos, influencian los procesos inflamatorios. Por ejemplo, en estudios clínicos, mayores concentraciones del ácido trans-palmitoléico (TP) coinciden con una menor adiposidad y un menor riesgo de desarrollar DT2. El objetivo de este estudio fue evaluar el efecto del TP sobre la expresión del inflamasoma NLRP3 en un modelo de obesidad inducida por dieta (OID) en roedores. Métodos: Se alimentaron ratones C57BL/6J ad libitum con una dieta control o alta en lípidos (AL), adicionada o no con TP (3 g/kg dieta), durante 11 semanas. Se cuantificó la concentración de IL-1β en elsuero de los animales, y en el tejido adiposo epididimal se midió la expresión de los componentes del inflamasoma. Resultados: A pesar del aumento de peso en ambos grupos de dieta con alto contenido en lípidos, el grupo alto en lípidos TP ganó menos peso que el grupo AL. Por otro lado, la expresión de genes del inflamasoma resultó mayor en los grupos AL, pero no se encontraron diferencias entre los grupos AL y AL TP. Además, no se observaron diferencias en la concentración de IL-1β en suero entre grupos. Conclusiones: La dieta suplementada con TP previno el aumento del peso corporal, pero no modificó la expresión de los componentes del inflamasoma ni la concentración de IL-1β en suero.

MicroRNA-150-5p inhibits the proliferation and invasion of human larynx epidermiod cancer cells though regulating peptidyl-prolyl cis/trans isomerase

Abstract Objective This study aimed to investigate the molecular mechanism of miR-150-5p regulating the malignant biological behavior of Human Epidermoid cancer cell (HEp-2) by targeting peptidyl-prolyl cis/trans isomerase NIMA-Interacting-1 (PIN1). Methods Firstly, qRT-PCR and Western blot were adopted to detect the expression levels of miR-150-5p and PIN1 in cancer tissue and paracancerous tissues of patients with LSCC, and those in human bronchial epithelial cells (16 HBE) and HEp-2. Next, the targeted relationship between miR-150-5p and PIN1 was assessed by bioinformatics website and dual-luciferase reporter assay, followed by their correlation analysis. Besides, after interfering with miR-150-5p or PIN1 expression in HEp-2 cells, CCK-8, cell colony formation assay, and transwell assay were utilized to detect the proliferation, viability, and invasion of cells, respectively. Subsequently, the protein levels of MMP-2, MMP-9, and EMT-related proteins in HEp-2 cells were checked by Western blot. Results Expression of miR-150-5p was down-regulated in LSCC tissues and HEp-2 cells. Moreover, miR-150-5p suppressed proliferation and invasion of HEp-2 cells, affected protein expression related to MMP and EMT, thereby inhibiting development of cancer. The expression of PIN1 was significantly increased in cancer tissues and HEp-2 cells, and there was a targeted relationship and negative correlation between miR-150-5p and PIN1 in cancer tissue. However, overexpression of PIN1 could reverse the effect of miR-150-5p on the proliferation and invasion of HEp-2 cells. Conclusion In a nutshell, there is a targeted relationship between PIN1 and miR-150-5p. Besides, miR-150-5p can inhibit the proliferation and invasion of HEp-2 cells by regulating the expression of PIN1. Level of evidence 3.

Experiencia actual en el abordaje laparoscópico transabdominal preperitoneal de hernias inguinales en el Servicio de Cirugía General del Hospital Nacional: periodo 2018-2022

Introducción: la hernia inguinal es uno de los principales motivos de consulta quirúrgica y su reparación es uno de los procedimientos más comunes en cirugía. Objetivo: determinar la experiencia en el abordaje laparoscópico de las hernias inguinales por técnica transabdominal preperitoneal en el Servicio de Cirugía General del Centro Médico Nacional-Hospital Nacional. Metodología: estudio observacional descriptivo retrospectivo de corte temporal transversal. En pacientes de 16 a 90 años de edad con diagnóstico de hernia inguinal internados en el Servicio de Cirugía General del Centro Médico Nacional-Hospital Nacional para hernioplastia electiva. Resultados: se llevaron a cabo 30 hernioplastias por técnica técnica trans-abdominal pre-peritoneal de los cuales el 73 % fue realizado en hombres y 27 % en mujeres; se identificó una media de edad de 48,4 años, el grupo etario con mayor frecuencia fue de 38 a 48 años. En el examen físico pre quirúrgico se encontraron hernias inguinales unilaterales en el 76.6 % y bilaterales en el 23.3 %; en la mayor parte de los pacientes las hernias fueron primarias en el 86.6 %y recidivada en el 13.3 %. El tiempo quirúrgico en promedio fue de 93.1 minutos; con un tiempo máximo de 120 minutos y mínimo de 60 minutos. El tiempo de hospitalización en el 100 % de los pacientes fue de 48 h. De las complicaciones post operatorias se establece que el 76.6 % no presento ningún tipo de complicación; el 20 % presentó seroma como complicación principal y 3.3 % infección del sitio quirúrgico. Conclusión: debido a su alta frecuencia y a su impacto en la incapacidad laboral y social, las hernias inguinales representan una de las patologías quirúrgicas más importantes con bajas tasas de complicaciones post operatorias y corta estancia hospitalaria.

Introduction: inguinal hernia is one of the main reasons TAPP, e-TEP (Totally extraperitoneal with extended vision) for surgical consultation and its repair is one of the most common surgical procedures. Objective: to determine the experience in the laparoscopic approach of inguinal hernias by preperitoneal transabdominal technique in the Servicio de Cirugía General of the Centro Médico Nacional-Hospital Nacional. Methodology: retrospective descriptive observational study of cross-sectional time. In patients from 16 to 90 years of age with a diagnosis of inguinal hernia admitted to the Servicio de Cirugía General of the Centro Médico Nacional-Hospital Nacional for elective hernioplasty. Results: 30 hernioplasties were carried out by the TAPP technique, of which 73 % were performed in men and 27 % in women; a mean age of 48.4 years was identified, the age group most frequently being 38 to 48 years. In the pre-surgical physical examination, unilateral inguinal hernias were found in 76.6 % and bilateral in 23.3 %; in most of the patients the hernias were primary in 86.6 % and recurred in 13.3 %. Average surgical time was 93.1 minutes; with a maximum time of 120 minutes and a minimum of 60 minutes. The hospitalization time in 100 % of the patients was 48 hours. Of the post-operative complications, it is established that 76.6% did not present any type of complication; 20 % presented seroma as the main complication and 3.3 % surgical site infection. Conclusion: due to its high frequency and its impact on work and social disability, inguinal hernias represent one of the most important surgical pathologies with low rates of postoperative complications and short hospital stay.

Obstáculos identificados por las personas trans en la accesibilidad a los consultorios inclusivos en la provincia de Buenos Aires / Barriers to access inclusive healthcare services identified by transgender people in Buenos Aires province

RESUMEN INTRODUCCIÓN El acceso a la salud para las personas trans ha sido históricamente obstaculizado por el estigma y la discriminación institucional. A pesar de los avances legales y sociales en materia de reconocimiento de derechos de los últimos años, todavía se identifican barreras para su efectivo cumplimiento. Este trabajo tuvo como objetivo describir las principales barreras en la accesibilidad a los consultorios inclusivos (CI) y analizar las necesidades específicas de la población trans que se atiende en la provincia de Buenos Aires (PBA). MÉTODOS Se presenta un subanálisis de un estudio cualitativo más amplio realizado en 2017, cuyo objetivo fue relevar información sobre demandas sanitarias, accesibilidad y calidad de atención de los servicios de salud específicos para población de lesbianas, gays, bisexuales y trans (LGBT) en ocho efectores de la PBA. La muestra fue intencional, conformada por 29 entrevistas semiestructuradas a referentes y profesionales y 2 grupos focales con masculinidades y feminidades trans, respectivamente. Se realizó un análisis temático. RESULTADOS Se identificaron barreras organizacionales y simbólicas, sobre todo en los CI que funcionan en hospitales. Entre las simbólicas, se evidencia la persistencia de prácticas patologizantes en salud mental. DISCUSIÓN Los CI brindan una respuesta satisfactoria, pero transitoria. Es necesario transversalizar la perspectiva de género a todas las intervenciones en salud.

ABSTRACT INTRODUCTION Access to health for trans people has historically been hampered by institutional stigma and discrimination. Despite the legal and social advances achieved in recent years in terms of recognition of rights, barriers to effective enforcement are still identified. The objective of this work was to describe the main barriers to access inclusive healthcare services and analyze the specific needs of trans people receiving healthcare in the province of Buenos Aires (PBA). METHODS A subanalysis of a wider qualitative study conducted in 2017 is presented, its objective was to collect information on healthcare needs, accessibility and quality of care of health services specific for lesbian, gay, bisexual and transgender (LGBT) population in eight healthcare providers of PBA. The sample was intentional, including 29 semi-structured interviews to social organization leaders and professionals, and 2 focus groups with trans masculinities and femininities, respectively. Data were analyzed using thematic analysis. RESULTS Organizational and symbolic barriers were identified, especially in inclusive healthcare services functioning in hospitals. One of the symbolic ones was the persistence of pathologizing practices in mental healthcare. DISCUSSION Inclusive healthcare services provide a satisfactory, but temporary response. It is necessary to adopt a cross-cutting gender perspective for all healthcare interventions.

Factores socioafectivos y su influencia en la actividad sexual de adolescentes / Socioemotional Factors and Their Influence on Sexual Activity Among Adolescents / Fatores socioafetivos e sua influência na atividade sexual de adolescentes

durante la adolescencia se adquieren conocimientos y se desarrollan actitudes hacia el sexo, la anticoncepción y la reproducción, aspectos que influirán en el comportamiento sexual que repercute directamente en la calidad de vida. Por tanto, el objetivo de este estudio fue determinar los factores sociales y emocionales asociados al inicio de la actividad sexual, así como sus consecuencias como el embarazo y las enfermedades de transmisión sexual en adolescentes y adultos jóvenes. Materiales y métodos: estudio descriptivo, transversal y correlacional de una muestra de 1191 estudiantes de secundaria que recibieron cuestionarios sobre factores socioemocionales e iniciación sexual. Los datos se procesaron mediante estadística descriptiva y la prueba chi-cuadrado de Pearson para determinar la asociación entre los factores evaluados. Resultados: la tasa de inicio de la actividad sexual entre los adolescentes fue del 45 %, de los cuales el 14 % utilizaba protección. El 88,8 % de los adolescentes dice recibir apoyo de los padres, el 39 % acepta tratos violentos en el hogar y el 47 % tuvo una madre adolescente embarazada, entre otros aspectos. Conclusiones: existe una relación significativa (con p < 0.05) entre los factores socioemocionales de género, proyectos de vida, actividades extracurriculares, apoyo de los padres, violencia intrafamiliar y maltrato, con respecto a las madres con antecedente de embarazo precoz e inicio de la actividad sexual en la adolescencia.

During adolescence, knowledge is acquired and attitudes toward sex, contraception, and reproduction are developed, aspects that will influence sexual behavior, which has a direct impact on quality of life. Therefore, this study was designed to determine the social and emotional factors associated with the onset of sexual activity and its consequences, such as pregnancy and sexually transmitted dis-eases, among adolescents and young adults. Materials and Methods: A descriptive, cross-sectional, and cor-relational study involving 1,191 high school students was conducted. A questionnaire containing questions on socio-emotional factors and sexual initiation was administered. Data were processed using descriptive statistics and Pearson's chi-square test to determine the association between the evaluated factors. Results:The initiation rate of sexual activity among adolescents was 45%, of whom 14% used protection. However, 88.8% of the adolescents received parental support, 39% accepted violent treatment at home, and 47% had a pregnant adolescent mother, among other aspects. Conclusions: There is a significant relationship between the socio-emotional factors of sex, life projects, extracurricular activities, parental support, intra-family violence, and mistreatment. Having a mother with a history of early pregnancy is associated with the onset of sexual activity among adolescents.

durante a adolescência, são adquiridos conhecimentos e desenvolvidas atitudes em relação ao sexo, contracepção e reprodução, aspectos que irão influenciar o comportamento sexual que afeta diretamente a qualidade de vida. Portanto, o objetivo deste estudo foi determinar os fatores sociais e emocionais associados ao início da atividade sexual, bem como suas consequências, como a gravidez e doenças sexualmente transmissíveis em adolescentes e jovens adultos. Materiais e métodos: estudo descritivo, transversal e correlacional em uma amostra de 1191 estudantes do ensino médio que rece-beram questionários sobre fatores socioemocionais e iniciação sexual. Os dados foram processados por meio de estatística descritiva e teste qui-quadrado de Pearson para determinar a associação entre os fatores avaliados. Resultados: a taxa de início da atividade sexual entre os adolescentes foi de 45%, dos quais 14% utilizavam proteção. 88,8% dos adolescentes afirmaram receber apoio dos pais, 39% recebem tratamento violento em casa e 47% têm mãe adolescente grávida, entre outros aspectos. Conclusões: existe uma relação significativa com p < 0,05 entre os fatores socioemocionais de gênero, projetos de vida, atividades extracurriculares, apoio parental, violência doméstica e maus-tratos. Mães com histó-rico de gravidez precoce e início da atividade sexual na adolescência.

Percepções e expectativas de homens trans acerca dos relacionamentos afetivo-sexuais pós-transição / Trans men's perceptions and expectations of post-transition affective-sexual relationships / Percepciones y expectativas de los hombres trans sobre las relaciones afectivo-sexuales después de la transición

As identidades transmasculinas ganharam visibilidade social e acadêmica no Brasil a partir de 2010, contudo, as questões subjetivas dos homens trans ainda são pouco debatidas, em particular temas associados aos relacionamentos afetivos na experiência desses sujeitos. Este estudo qualitativo tem por objetivo identificar as percepções e expectativas dos homens trans acerca dos relacionamentos afetivo-sexuais no cenário pós-transição de gênero. Participaram da pesquisa 15 homens transexuais hormonizados, com idades entre 20 e 41 anos. A coleta de dados foi realizada por meio de entrevista semiestruturada nas modalidades presencial e on-line. Empregou-se análise temática reflexiva, que resultou em dois temas analíticos. Os resultados apontam que os homens trans, ao contrário de suas expectativas iniciais, percebem que tiveram menos oportunidades de relacionamentos afetivo-sexuais depois de sua transição de gênero. Os participantes atribuem essa dificuldade especialmente ao fato de não terem se submetido à cirurgia de redesignação sexual. O desconforto é acentuado por sua materialidade corpórea divergente da cisnormatividade, sistema regulador que associa pessoas pertencentes ao gênero masculino à presença de um pênis. Outra fonte de desconforto é o repúdio social, que alimenta a abjeção, exotização e fetichização dos corpos transmasculinos. Também são descritas as especificidades do relacionamento dos homens trans com mulheres cisgênero, heterossexuais e lésbicas. Os resultados evidenciam que a fixação persistente no genital, como referente e signo determinante do gênero e da sexualidade, modula e regula a busca e o encontro de parceira(o) íntima(o).(AU)

Transmasculine identities have gained social and academic visibility in Brazil since 2010, but subjective issues, especially those associated with affective relationships, are still little discussed. This qualitative study sought to identify trans men's perceptions and expectations regarding post-transition affective-sexual relationships. A total of 15 transsexual men undergoing hormone therapy, aged between 20 and 41 years, participated in the research. Data were collected by means of in-person and online semi-structured interviews and analysed using reflexive thematic analysis, which resulted in two analytical themes. Results show that trans men, differently from their initial expectations, perceive fewer opportunities for affective-sexual relationships after their gender transition. The participants attribute this difficulty, especially, to the fact that they have not undergone sexual reassignment surgery. Discomfort isaccentuated by their bodily materiality diverging from cisnormativity, the regulatory system that associates people belonging to the male gender with the presence of a penis. Another source of discomfort is the social repudiation, which reinforces the abjection, exoticization, and fetishization of transmasculine bodies. The specifics of trans men's relationships with cisgender, heterosexual, and lesbian women are also described. The results show that the persistent fixation on the genital, as a referent and determinant sign of gender and sexuality, modulates and regulates the search for and encounter of intimate partners.(AU)

Las identidades transmasculinas han ganado visibilidad social y académica en Brasil desde 2010, sin embargo, las cuestiones subjetivas de los hombres trans son aún poco discutidas, en particular las cuestiones asociadas a las relaciones afectivas en la experiencia de estos sujetos. Este estudio cualitativo tiene como objetivo identificar las percepciones y expectativas de los hombres trans sobre las relaciones afectivo-sexuales después de la transición de género. Participaron en la investigación 15 hombres transexuales hormonados, de edades comprendidas entre los 20 y los 41 años. La recopilación de datos se realizó mediante una entrevista semiestructurada en las modalidades presencial y en línea. Se realizó un análisis temático reflexivo, que dio como resultado dos temas analíticos. Los resultados muestran que los hombres trans, al contrario de sus expectativas iniciales, perciben que han tenido menos oportunidades de relaciones afectivo-sexuales después de su transición de género. Los participantes atribuyen esta dificultad especialmente al hecho de no haberse sometido a cirugía de reasignación sexual. La incomodidad se acentúa por su materialidad corpórea divergente de la cisnormatividad, un sistema normativo según el cual las personas pertenecientes al género masculino deben tener pene. Otra fuente de malestar es el repudio social, que alimenta la abyección, la exotización y la fetichización de los cuerpos transmasculinos. También se describen las especificidades de las relaciones de los hombres trans con las mujeres heterosexuales, cisgénero y lesbianas. Los resultados muestran que la persistente fijación en los genitales, como referente y signo determinante del género y la sexualidad, modula y regula la búsqueda y el encuentro de parejas íntimas.(AU)

"Una cosa es la ley, y otra la práctica médica": Las adolescencias trans en el sistema de salud en Argentina: entre las narrativas de derechos y el adultocentrismo / "One thing is the law, and another the medical practice". Trans adolescents in the health system in Argentina: between the narratives of rights and adultcentrism / "Uma coisa é a lei e outra é a prática médica". Adolescentes trans no sistema de saúde da Argentina: entre narrativas de direitos e adultocentrismo

Resumen En Argentina, la legislación garantiza que las infancias y adolescencias trans puedan realizar el cambio de nombre y sexo en su documentación de acuerdo con su identidad autopercibida. Antes de los 16 años, deben presentar conformidad a través de sus representantes legales y contar con la asistencia de unx abogadx del niñx. Distintos espacios gubernamentales han construido protocolos de atención de salud integral despatologizantes que garantizan el acceso a procesos de modificación corporal con autonomía. Sin embargo, en la configuración de las intervenciones médicas, las narrativas de derechos se traman con la persistencia del cisexismo y del adultocentrismo. La autoridad epistémica de la ciencia, o la especialidad, suele anteponerse a las decisiones de lxs adolescentes. Abordo este problema a través del análisis de protocolos oficiales y entrevistas a profesionales de la salud en una ciudad intermedia de Buenos Aires.

Abstract In Argentina, the legislation guarantees that trans children and adolescents can change their name and sex in their documentation in accordance with their self-perceived identity. Before the age of 16, they must present conformity through their legal representatives and have the assistance of a child's lawyer. Different governmental spaces have built comprehensive depathologizing health care protocols that guarantee access to body modification processes with autonomy. However, in the configuration of medical interventions, the narratives of rights are woven with the persistence of cissexism and adultcentrism. The epistemic authority of science, or specialty, often takes precedence over the decisions of adolescents. I approach this problem through the analysis of official protocols and interviews with health professionals in an intermediate city in the province of Buenos Aires.

Resumo Na Argentina, a legislação garante que crianças e adolescentes trans possam mudar seu nome e sexo em sua documentação de acordo com sua identidade autopercebida. Antes dos 16 anos de idade, eles devem apresentar seu consentimento por meio de seus representantes legais e ter a assistência de um advogado infantil. Diferentes espaços governamentais criaram protocolos integrais de atendimento à saúde despatologizantes que garantem o acesso a processos de modificação corporal com autonomia. Entretanto, na configuração das intervenções médicas, as narrativas de direitos são tecidas com a persistência do cissexismo e do adultocentrismo. A autoridade epistêmica da ciência, ou especialidade, muitas vezes tem precedência sobre as decisões dos adolescentes. Abordo esse problema por meio da análise de protocolos oficiais e entrevistas com profissionais de saúde em uma cidade intermediária de Buenos Aires.

Emotional States, Adherence and Prevention Behaviors in Trans Women during the COVID-19 Lockdown / Estados Emocionais e Comportamentos de Aderência e Prevenção em Mulheres Trans Durante o Confinamento por COVID-19 / Estados Emocionales y Conductas de Adherencia y Prevención en Mujeres Trans Durante el Confinamiento por COVID-19

Abstract The pandemic generated multiple biopsychosocial challenges that significantly impacted the health of trans women. The aim of this study is to characterize emotional states and resilience in trans women in Colombia and their relationship to COVID-19 adherence behaviors and prevention behaviors during lockdown. The study employed a cross-sectional descriptive correlational design, with an anonymous online questionnaire that was given to 100 trans women in five cities in Colombia. During the pandemic, more than half of trans women experienced negative emotional states such as depression, anxiety and somatization, and some positive ones such as resilience and personal growth. The latter two contribute to reinforcing adherence and prevention behaviors. This study demonstrates a clear need to give priority and recognition to trans women and their health needs, in order to protect their rights, their well-being, and their health.

Resumo A pandemia gerou múltiplos desafios biopsicossociais que impactaram significativamente a saúde das mulheres trans. O objetivo deste estudo é caracterizar estados emocionais e resiliência em mulheres trans da Colômbia e sua relação com os comportamentos de aderência à COVID-19 e os comportamentos de prevenção durante o confinamento. Foi realizado um desenho descritivo, transversal e correlacional, foi administrado um questionário online anônimo a 100 mulheres trans em cinco cidades da Colômbia. Durante a pandemia, mais da metade das mulheres trans experimentaram estados emocionais negativos como depressão, ansiedade e somatização, e alguns positivos como a resiliência e o crescimento pessoal. Estes dois últimos contribuem para reforçar os comportamentos de adesão e de prevenção. Em situações como a pandemia, é prioritário dar reconhecimento às mulheres trans e às suas necessidades de saúde, a fim de proteger os seus direitos e a sua saúde integral.

Resumen La pandemia generó múltiples desafíos biopsicosociales que impactaron significativamente en la salud de las mujeres trans. El objetivo del presente estudio fue caracterizar los estados emocionales y resiliencia en mujeres trans de Colombia y su relación con las conductas de adherencia y prevención a la COVID-19 durante el confinamiento. Se realizó un diseño trasversal correlacional descriptivo, administrando un cuestionario online anónimo a 100 mujeres trans en cinco ciudades de Colombia. Se encontró que más de la mitad de las mujeres trans han experimentado estados emocionales negativos como depresión, ansiedad y somatización y algunos positivos como resiliencia y crecimiento personal. Los dos últimos contribuyen a fortalecer las conductas de adherencia y prevención de la COVID-19. Es prioritario dar reconocimiento a las mujeres trans y a sus necesidades de salud, desde diversos frentes de acción con el fin de proteger sus derechos y su salud integral.

Exposição ambiental ao benzeno, parâmetros hematológicos e níveis urinários de ácido trans, trans-mucônico em uma comunidade da região Nordeste do Brasil / Environmental exposure to benzene, hematological parameters, and urinary levels of trans,trans-muconic acid in a community in the Northeast region of Brazil

Resumo Introdução O metabólito do benzeno ácido trans, trans-mucônico (t, t-MA) urinário é considerado bom bioindicador de exposição a níveis de benzeno no ar superiores a 0,50 ppm. Objetivo Determinar níveis urinários de ácido trans, trans-mucônico (t, t-MA) e investigar relação com potenciais fontes de exposição ao benzeno e associação com parâmetros hematológicos. Método Estudo seccional, com 150 moradores de Açailândia, Maranhão, Brasil. Utilizou-se questionário estruturado para caracterização sociodemográfica, ocupacional, dentre outras. Coletaram-se amostras de urina e sangue para determinar níveis de t, t-MA e realização de hemograma. As associações foram exploradas mediante regressão logística e linear múltipla. Resultados O t, t-MA foi detectado em 27% dos participantes, sendo a média da concentração 0,15 mg/g de creatinina. As alterações hematológicas mais frequentes foram bastonetes baixos (41%), eosinofilia (33%) e níveis reduzidos de hemoglobina (19%). Não encontrou-se correlação estatisticamente significativa entre t, t-MA e parâmetros do hemograma. Os fatores que influenciaram de forma significativa a excreção de t, t-MA foram cor da pele, prática regular de atividades de lazer com exposição a solventes orgânicos e consumo de refrigerante e refresco nas últimas 24 horas. Conclusão A população parece não estar exposta a concentrações elevadas de benzeno. Entretanto, o perfil hematológico revelou presença de alterações bioquímicas compatíveis com anemia e comprometimento imunológico.

Abstract Background The urine benzene metabolite trans,trans-muconic (t,t-MA) is considered a good bioindicator of exposure to benzene levels in the air above 0.50 ppm. Objective Determine urinary levels of trans, trans-muconic acid (t,t-MA) and investigate the relationship with potential sources of exposure to benzene and its association with hematological parameters. Method A sectional study was carried out with 150 residents of Açailândia, Maranhão, Brazil. A structured questionnaire was used for sociodemographic and occupational characterization, among others. Urine and blood samples were collected to determine t,t-MA levels and blood counts. Associations were explored using multiple linear and logistic regressions. Results The t,t-MA was detected in 27% of the participants, with an average concentration of 0.15 mg/g creatinine. The most frequent hematological changes were low rods (41%), eosinophilia (33%), and reduced hemoglobin levels (19%). No statistically significant correlation was found between t,t-MA and blood count parameters. The factors that significantly influenced the excretion of t, t-MA were skin color, regular practice of leisure activities with exposure to organic solvents, and consumption of soda and soft drinks in the last 24 hours. Conclusion The population does not appear to be exposed to high concentrations of benzene. However, the hematological profile revealed the presence of biochemical changes compatible with anemia and immunological impairment.

Long-term Recovery Patterns of Olfactory Function after Trans-sphenoidal Approach with Nasoseptal Flap Elevation

Abstract Introduction Nasoseptal flap is widely used in reconstruction of the skull base to prevent cerebrospinal fluid leakage after surgery for skull base lesions. There has been a debate on whether more severe olfactory dysfunction occurs after nasoseptal flap elevation than the conventional trans-sphenoidal approach. Objective To compare the long-term recovery patterns associated with nasoseptal flap and the conventional trans-sphenoidal approach. Methods The subjects were divided into the conventional trans-sphenoidal approach group and the nasoseptal flap elevation group. We followed up self-reported olfactory score using the visual analogue scale and threshold discrimination identification (TDI) score of the Korean Version of the Sniffin Stick test II for 12 months, with olfactory training. Results The study included 31 patients who underwent the trans-sphenoidal approach. Compared with preoperative status, the mean visual analogue scale and TDI scores in the conventional trans-sphenoidal approach group recovered 2 months postoperatively, while in the nasoseptal flap elevation group the visual analogue scale and TDI scores recovered 6 months and 3 months after surgery, respectively. Twelve months after surgery, the visual analogue scale and TDI scores in the conventional trans-sphenoidal approach group were 9.3 ± 0.5 and 28.5 ± 4.3, while those from the nasoseptal flap elevation group were 8.9 ± 1.5 and 27.2 ± 4.7 (p = 0.326; 0.473). Only one of the patients in the nasoseptal flap elevation group had permanent olfactory dysfunction. Conclusion The olfactory function recovered more gradually in the nasoseptal flap elevation group than in the conventional trans-sphenoidal approach group, but there was no difference between the two groups after 6 months.

"La covid no ha podido con nosotrxs": impacto de la pandemia en el acceso efectivo de personas trans a tratamientos hormonales integrales en Córdoba, Argentina / "Covid couldn't get us down": pandemic impact on effective access to transgender hormone therapy in Cordoba, Argentina

Resumen El objetivo de este trabajo fue indagar el impacto de la pandemia de la covid-19 en el acceso de personas trans a tratamientos hormonales en los servicios de salud pública de la provincia de Córdoba, Argentina. En los meses de junio/julio de 2020, se realizaron 16 entrevistas telefónicas a personas trans en proceso de hormonización y usuarias de servicios públicos de salud, a profesionales sanitarios de dispositivos específicos de atención a personas trans, integrantes de organizaciones sociales y de gestión. Se aplicó un análisis temático que identificó tres temas y subtemas: 1) Estar a la deriva en relación al tratamiento hormonal: Reestructuración de servicios de salud; Sentimientos de incertidumbre; 2) Lo necesito, el cuerpo lo pide: suministro de hormonas: Significados en torno al tratamiento; Estrategias y resistencias; 3) Oportunidad de visibilizar fallas estructurales: las hormonas como la punta del iceberg: Déficit en las políticas públicas: en pandemia desde hace muchos años; Exclusiones del CIStema de salud. Se concluyó que la pandemia acarreó un grave retroceso para los derechos de las personas trans. Sin embargo, se identificaron estrategias claves de acceso, vinculadas principalmente a las organizaciones y activistas trans y travestis.

Abstract The purpose of this research was to study the impact of covid-19 pandemic on transgender people's access to healthcare and hormone gender-affirming treatments in public healthcare services of the province of Córdoba, Argentina. Between June/July-2020, sixteen telephone interviews were conducted with people in gender-affirming hormone treatments in public healthcare services, professionals, members of social organizations and management. A thematic analysis was carried out, identifiying three themes and sub-themes: 1) Being adrift in relation to hormonal treatment: Restructuring of healthcare services; Feelings of uncertainty. 2) I need it, the body asks for it: supply of hormones: Meanings around treatment; Strategies and resistance. 3) Opportunity to make visible structural failures: hormones as the tip of the iceberg: Deficit in public policies: in a pandemic for many years; Exclusions from the CISHealthcare System. We conclude that the pandemic implied a serious setback for transgender people's rights. However, strategies were identified, mainly linked to transgender organizations and activists, which were key to access.