RESUMO
Objective To study whether hTR antisense PS ODN has anticancer effect and the effect of increasing the susceptibility of tumor to DDP, we explore the effect of hTR antisense oligodeoxynucleotide in combination with DDP on human gastric carcinoma transplanted subcutaneously in nude mice in vivo. Methods The model of human gastric carcinoma transplanted subcutaneously was established in thirty nude mice, then divided randomly into 5 groups (Control group, ASODN group, RODN group, DDP group and ASODN+DDP group). The weight of nude mice was measured, and the tumor growth inhibitory rate was calculated. The relative telomerase activity was quantitatively measured by TRAP PCR ELISA methods. Results The maximum tumor inhibitory rate in ASODN+DDP group, ASODN group, DDP group and RODN group was 94.2 %, 84.3 %, 92.8 % and 26.9 % respectively. There was significant difference in the relative telomerase activity among four treatment groups. Conclusions The results suggested that hTR antisense PS ODN may act as a specific tumor growth inhibitor and telomerase activity inhibitor may be in sequence specific manner, and have the effect of increasing the susceptibility of transplanted tumor to DDP.
RESUMO
Objective:To explore the effect of c-raf-1 antisense oligodeoxynucleotides (ASODN) treatment in the human ovarian epithelial cancer transplanted subcutaneously in nude mice.Methods:The models of human ovarian epithelial cancer transplanted subcutaneously were established in 15 nude mice,then divided randomly into 3 groups and different treatment were given respectively (control group,senseexperimental group and antisense experimental group).The weight of nude mice and tumor volume were observed,the tumor growth inhibitory rate and the tumor response rate calculated,too.Results:The growth inhibitory rate in sense experimental group and antisense experimental group were 6.8% and 68.1%,respectively,the tumor response rate of antisense experimental group was 16.7%.There was no statistical difference in nude mice weight among the 3 groups.Conclusion:The results suggest that there is a positive value in the human ovarian epithelial cancer transplanted subcutaneously in nude mice treated by c-raf-1 antisense oligodeoxynucleotides,which will be an important gene therapeutic strategy for the ovarian epithelial carcinoma in the future.