RESUMO
Objective:To obtain flavonoids of Cacumen Platycladi with high purity by using the method of ultrasonic emulsification combined with ultrafiltration membrane separation.Methods:Seven compounds including myricetrin as the reference substances,an HPLC method was used for the content determination.Using the single factor experiments,ultrasound,microwave,reflux and ultrasonic emulsification were compared,and using the orthogonal experiments,the ultrasonic emulsification time,ethanol concentration,solid-liquid ratio and times were studied.Biomax-5 membrane was adopted to improve the membrane separation.Results:The single factor experiments showed the homogeneous extraction method with the highest contents.The orthogonal experiments showed the optimal extracting conditions as follows:the ultrasonic emulsification time was 15 min,the ethanol concentration was 50%,the material-liquid ratio was 1∶10,and the extraction times was 3.The conditions of ultrafiltration membrane separation were as follows:the flow rate was 1.5 L·min-1,and the membrane separation pressure was 0.8 kg.Conclusion:The combination of ultrasonic emulsification and ultrafiltration membrane separation is feasible in the extraction and purification of flavonoids in Cacumen Platycladi,and the product is with high purity,suggesting the method has good application prospect.
RESUMO
Objective: To study the application of four kinds of hollow fiber ultrafiltration membranes by taking Qihong Maitong Injection (QMI) microfiltrated liquid as the research object. Methods: Polysulfone, polyether sulfone, polypropylene, and blended composite membranes with the entrapment relative molecular mass of 100000 for ultrafiltration were selected to determine the best appropriate ultrafiltration membrane material. The membrane flux of ultrafiltration was determined by taking the content of active ingredients (Astragalus total saponin, astragaloside IV, and hydroxy safflower yellow A), solid reduction rate, protein reduction rate, related substances, and pyrogen inspection of different membrane materials as the evaluation indexes. Results: The suitability of four different kinds of ultrafiltration membrane materials with the same entrapment relative molecular mass was different. The pure water flux recovery rates of polypropylene, polyether sulfone, and blended composite materials are higher than that of polysulfone material. The component permeation rates of polypropylene and polyether sulfone materials were higher, while the solid and protein reduction rates of polysulfone and blend compound materials were higher. For QMI, the ultrafiltration membrane with entrapment relative molecular mass of 100000 could effectively remove the pyrogen. Conclusion: The polypropylene-100000 ultrafiltration membrane could not only effectively remove the solid and high polymer material, but also keep the active ingredients. It is suitable for the purification of QMI.
RESUMO
Objective: To investigate the ultrafiltration effect of polysulfone hollow fiber ultrafiltration membrane and polysulfone plate ultrafiltration membrane with relative molecular weight by different cut-off on the decarburization fluid of Reduning Injection. Methods: The transfer rates of chlorogenic acid and geniposide, fingerprint similarity, and removal of bacterial endotoxin, protein, oxalate, and resin before and after ultrafiltration were used as inspect indicators to optimize the purification technology for the decarburization fluid of Reduning Injection. Results: The obvious effect of polysulfone hollow fiber ultrafiltration membrane with the relative molecular weight 10000 on oxalate and resin in the decarburization fluid of Reduning Injection was observed. Bacterial endotoxin (100%) in the decarburization fluid of Reduning Injection was removed by the polysulfone plate ultrafiltration membrane with the relative molecular weight 5000. Conclusion: The stability and the preparation safety of Reduning Injection could be ensured by the two stage combination of the ultrafiltration membrane technology.
RESUMO
Objective: To study the best pre-treatment method and its technology before ultrafiltration of Reduning water extract. Methods: To investigate such pre-treatment methods as chitosan flocculation on the impurity of prescription water extract, as well as polysulfone ultrafiltration membrane refination effect with molecular weight cut-off three to five million. Results: The centrifugation and decompress filtration were not benefit to promote the geniposide reservation and reduce fouling degree of membrane. Absorption of active carbon could make the geniposide reservation deeply lower. The geniposide was reserved mostly and the total extracts reduced mostly for chitosan flocculation and microfiltration. But the flux was higher and the membrane fouling is smaller after chitosan flocculation. Conclusion: Chitosan flocculation is the best pre-treatment method. The operating conditions are that temperature of Reduning water extract is 70°C, pH value 6, and the dosage of 1% chitosan is 70 mL/L.
RESUMO
Objective: In order to improve the technology of Chinese medicine extraction, experiments were made with large pore size ultrafiltration (LPS UF) membranes in the process of Chinese medicine production, and the feasibility of replacing traditional alcohol sedimentation with LPS UF method in the production of compound Chinese medicine was also explored. Methods: The water extraction liquid of Shenbao Mixture with alcohol percolation extract was ultrafiltered. Icariin as index component was determined. Results: Component of Icariin was reserved as 90%, this technology was similar to alcohol extraction. Conclusion: The experiments on the extraction of “Shengbao” mixture show that the LPS UF membranes with MWCO above 100,000 were more effective in retaining the effective ingredients and removing the precipitates.