RESUMO
Valeriana amurensis Smir. ex Kom. widely distributed in the northeast region of China and some region in Russia and Korea, and its underground parts (roots and rhizomes) being used to cure nervous system diseases such as insomnia. The active components including the essential oil and iridoids of underground parts were investigated in different harvest periods in order to evaluate the quality for the roots and rhizomes of V. amurensis. The content of the essential oil was obtained by hydrodistillation and bornyl acetate in the oil was quantitated by GC-EI. The iridoids, valepotriates were determined by potentiometric titration and the main component, valtrate was quantitated by HPLC-UV. The factors of biomass were considered in the determination of collection period. Statistical analysis of results showed that, the highest content of the essential oil per plant was 22.69 µl in withering period and then 21.58 µl in fruit ripening period, while the highest contents of bornyl acetate, valepotriates and valtrate per plant were 2.82 mg, 31.90 mg and 0.98 mg in fruit ripening period separately. Fruit ripening period was decided as the best harvest period for the content of active constituents and output of drug, and it would provide scientific basis for the artificial cultivation of V. amurensis.
Valeriana amurensis Smir. ex Kom. Se distribuye ampliamente en la regioÌn noreste de China y en algunas regiones de Rusia y Corea, y sus partes subterraÌneas (raiÌces y rizomas) se utilizan para curar enfermedades del sistema nervioso como el insomnio. Se investigaron los componentes activos, incluidos el aceite esencial y los iridoides de las partes subterraÌneas de V. amurensis en diferentes periÌodos de cosecha para evaluar la calidad de las raiÌces y rizomas. El contenido del aceite esencial se obtuvo mediante hidrodestilacioÌn y el acetato de bornilo en el aceite se cuantificoÌ por GC-EI. Los iridoides, valepotriatos se determinaron mediante valoracioÌn potenciomeÌtrica y el componente principal, el valtrato se cuantificoÌ por HPLC-UV. Los factores de biomasa fueron considerados en la determinacioÌn del periÌodo de recoleccioÌn. El anaÌlisis estadiÌstico de los resultados mostroÌ que el mayor contenido de aceite esencial por planta fue de 22,69 µl en el periÌodo de marchitacioÌn y luego de 21,58 µl en el periÌodo de maduracioÌn de la fruta, mientras que el mayor contenido de acetato de bornilo, valepotriatos y valtrato por planta fue de 2.82 mg, 31.90 mg y 0,98 mg, respectivamente, en el periÌodo de maduracioÌn de la fruta por separado. Se definioÌ el periÌodo de maduracioÌn de la fruta como el mejor periÌodo de cosecha para el contenido de constituyentes activos y la produccioÌn de droga, lo cual proporcionariÌa una base cientiÌfica para el cultivo artificial de V. amurensis.
Assuntos
Valeriana/química , Óleos Voláteis/química , Raízes de Plantas/química , Estações do Ano , Canfanos/análise , Cromatografia Líquida de Alta Pressão , Espectrometria de Massas por Ionização por Electrospray , Rizoma/química , Iridoides/análiseRESUMO
This study was aimed to establish an HPLC method for simultaneous content determination of valtrate, acevaltrate, and their degradation products, which were baldrinal and 11-ethoxyviburtinal, in Valeriana jatamansi Jones. The separation and quantification of 4 constituents mentioned above were performed on an Agilent ZORBAX SB-C18 (4.6 mm×250 mm, 5 μm). The mobile phase consisted of water (A) - acetonitrile (B) with an optimized gradient program. The flow rate was 1 mL·min-1. The column temperature was set at 25℃. The wavelength was set at 241 nm. And the injection volume was 10μL. The results showed that among 14 different places of V. jatamansi, the 4 contents determined were different. The contents of valtrate, acevaltrate, and baldrinal in the Yunnan Baoshan Mount were the highest. And the content of 11-ethoxyviburtinal was the highest in Yunnan Dali. It was concluded that the method was with good precision, reproducibility and stability. And it was suitable for the determination of 4 valepotriates ingredients in V. jatamansi. It also provided references for the quality control and exploitation of V. jatamansi.