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Mem. Inst. Oswaldo Cruz ; 103(3): 301-305, May 2008. ilus, tab
Artigo em Inglês | LILACS | ID: lil-485225

RESUMO

Three Enterococcus faecalis and one Enterococcus faecium strains were characterized by plasmid profile, pulsed-field gel electrophoresis (PFGE) and determination of antimicrobial minimal inhibitory concentrations. VanA elements were characterized by Long PCR, overlapping PCR and DNA sequencing. Enterococcal strains showed resistance to vancomycin and harbored the vanA gene, and three these were teicoplanin susceptible while one showed intermediate resistance to teicoplanin. Two E. faecalis strains showed indistinguishable PFGE profile while the third was unrelated. E. faecalis strains showed a deletion in the right terminal region of the Tn1546-like element. The E. faecium strain showed an insertion element in the vanXY intergenic region. Mutations in VanA elements were not found. Rearrangements in the VanA element could be responsible for incongruities in genotype and phenotype in these strains.


Assuntos
Humanos , Antibacterianos/farmacologia , Enterococcus faecalis , Enterococcus faecium , Teicoplanina/farmacologia , Vancomicina/farmacologia , Brasil , Proteínas de Bactérias/genética , Carbono-Oxigênio Ligases/genética , DNA Bacteriano/genética , Eletroforese em Gel de Campo Pulsado , Enterococcus faecalis/efeitos dos fármacos , Enterococcus faecalis/genética , Enterococcus faecalis/isolamento & purificação , Enterococcus faecium/efeitos dos fármacos , Enterococcus faecium/genética , Enterococcus faecium/isolamento & purificação , Genótipo , Testes de Sensibilidade Microbiana , Fenótipo , Reação em Cadeia da Polimerase , Análise de Sequência de DNA , Resistência a Vancomicina/genética
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