Chemical constituents and bioactive activities of Eleutherine americana / 中草药

Objective: To study the chemical constituents of Eleutherine americana. Methods: The compounds were isolated by various chromatographic techniques, including silica gel, TLC, sephadex LH-20, and semi-preparative HPLC, and their structures were identified by their physicochemical properties and 1H-NMR and 13C-NMR data. Results: Fifteen compounds were isolated from E. americana, which were germacrenin B (1), phaffiaol (2), 4,8-dihydroxy-3-methoxy-l-methylonthra-9,10-quinone-2-carboxylic acid methyl ester (3), 3-heptadecyl-5-methoxyphenol (4), ethyl linoleate (5), 3,5-dimethoxybiphenyl-4’-ol (6), karwinaphthol A (7), 5-hydroxylkarwinaphthol A (8), 3,4-dimethoxy-8-hydroxy-1-methyl-anthra-9,10-quinone-2-carboxylic acid methyl ester (9), isoeleutherin (10), eleutherin (11), isoeleutherol (12), naphtho-γ-lactone (+)-9-hydroxyeleutherol (13), senkyunone (14), and palmitoleic acid (15). Conclusion: Compounds 2, 4-7, and 13-15 were isolated from the genus for the first time. The compounds 3, 8, and 13 had strong vasodilator effects with diastolic rate of 85.3%, 81.8%, and 89.5%, respectively, which were basically equivalent to the positive drug of tanshinone IIA (86.3%).

Study on vinegar-processing of Corydalis Rhizoma based on component analysis and activity evaluation / 中草药

Objective: To investigate the vinegar-processing technology of Corydalis Rhizoma under the modern alcohol extraction process conditions and to provide the basis for explaining the vinegar-processing significance of Corydalis Rhizoma. Methods: The contents of 10 alkaloids (allocryptopine, sea papaverine hydrochloride, berberine, palmatine, berberine, dehydrogenation corydaline, tetrahydropalmatine, corydaline, tetrahydro coptisine, and dehydrogenation sea papaverine) extracted from the viniger-processed Corydalis Rhizoma (VCR) and raw Corydalis Rhizoma (RCR) were analyzed, and the vasodilatation activity of intestinal absorption was compared. Results: Among the contents of 10 alkaloids in VCR and RCR, four of them increased while six of them decreased, and the vasodilatation activity of intestinal absorption of VCR was little higher than that of RCR, there was no obvious difference. Conclusion: In the view of the correlation between the content of the 10 compounds and the vasodilatation activity of VCR and RCR, there are not direct relationship.