RESUMO
Objective: To clarify the contribution of the fingerprint peaks from different parts of prepared Ershen Pill to the effect of warming spleen to relieve diarrhea, and to reflect the material basis. Methods: The HPLC fingerprints of different parts of Ershen Pill (composed of Psoraleae Fructus stir-baked with saltwater and Myristica Fragrans simmered with bran) were established. The diarrhea index of acute diarrhea in mice caused by senna leaf was researched to compare the effect of warming spleen to relieve diarrhea. Results: The fingerprints of different parts of Ershen Pill were established, and all of the similarity degrees were over 0.90. The effect of warming spleen to relieve diarrhea of Ershen Pill was obtained from many constituents collaboratively. The contribution of different peaks to relieve diarrhea with astringents was ranked as 6 > 18 > 7 > 20 > 14 > 21 > 1 > 25 > 24 > 23 > 4 > 3 > 8 > 11. Conclusion: The method for establishing HPLC fingerprints of different parts of Ershen Pill is simple and repeatable. The relationship between the fingerprints and the effect of warming spleen to relieve diarrhea is paralleled to some extent. And this will lay a foundation for the research of processing mechanism of Ershen Pill.