Blood group changes in acute myeloid leukemia and their relationship with therapeutic effect / 中国输血杂志

【Objective】 To explore the blood group changes of two acute myeloid leukemia patients with suspected O type, and their relationship with the therapeutic effect. 【Methods】 Serological analysis of ABO blood group of patients was carried out by microcolumn gel method, tube method and absorption-elution test, ABO blood group genotyping was performed by microfluidic chip method. Exons E2 to E7 of ABO gene were amplified by PCR and sequenced by Sanger method. 【Results】 The forward typing of two cases were both O type, but the reverse typing were both A type. The absorption-elution test results all showed detection of antigen A. ABO gene phenotype of the two cases were both A, with genotyping results as A102/A102 and A102/O01, respectively. Sequencing results showed that SNP sites of ABO blood group were 467T/T, 261G/delG and 467C/T, respectively.In one case, the intensity of anti-A agglutination reaction changed significantly from weak to strong with the progress of treatment. 【Conclusion】 For clinical samples of acute myeloid leukemia patients with ABO forward and reverse typing discrepancy and suspected O type, the result of reverse typing should be valued, and absorption-elution test should be performed to further confirm the ABO blood type combining the genetic test results, so as to develop appropriate blood transfusion strategies for patients.

Effects of using different root canal sealers and protocols for cleaning intraradicular dentin on the bond strength of a composite resin used to reinforce weakened roots / Efectos del uso de diferentes selladores de conductos radiculares y protocolos para limpiar la dentina intrarradicular sobre la fuerza de unión de una resina compuesta utilizada para reforzar raíces debilitadas

Background: This study evaluated the effects of using different root canal sealers and protocols for cleaning intraradicular dentin on the bond strength of a composite resin used to reinforce weakened roots. Material and Methods: Sixty-four roots of extracted human maxillary canines were weakened, prepared and filled with two different endodontic sealers (Endofill and AH Plus). In half of the sample, set aside for each respective sealer, excess filling material was cleaned. In the other half, the weakened areas were not cleaned, and the excess of sealer was spread on the intraradicular dentin. Intentionally worn areas inside each root were restored with a microhybrid light-cure composite resin (Z100) to reinforce them, with and without acid etching. Prefabricated metal posts were fixed with a dual resin cement (RelyX ARC), and the specimens were submitted to a pull-out test. Statistical analysis was performed by means of Shapiro-Wilk, analysis of variance (one-way ANOVA) and Tukey-Kramer tests (p<0.05). Results: The groups filled with Endofill (GI, GII, GIII, GIV) had the lowest bond strength values, which were similar among each other (p>0.05).The greatest bond strength values were observed in roots filled with AH Plus (GV, GVI, GVII, GVIII), mainly without cleaning of the weakened areas, and followed by acid etching (GVII), and also with cleaning of the weakened areas, however, with no acid etching (GVI) (p<0.05). Conclusion: The greatest bond strength values were observed in roots filled with AH Plus; (1) without cleaning of the weakened areas and with acid etching, and; (2) with cleaning of the weakened areas, but without acid etching.

Antecedentes: este estudio evaluó los efectos del uso de diferentes selladores de conductos radiculares y protocolos para limpiar la dentina intrarradicular sobre la fuerza de unión de una resina compuesta utilizada para reforzar las raíces debilitadas. Material y Métodos: Sesenta y cuatro raíces de caninos maxilares humanos extraídos fueron debilitadas, preparadas y rellenadas con dos selladores endodónticos diferentes (Endofill y AH Plus). En la mitad de la muestra, reservada para cada sellador respectivo, se limpió el exceso de material de relleno. En la otra mitad, las áreas debilitadas no se limpiaron y el exceso de sellador se esparció sobre la dentina intrarradicular. Las áreas desgastadas intencionalmente dentro de cada raíz se restauraron con una resina compuesta fotopolimerizable microhíbrida (Z100) para reforzarlas, con y sin grabado ácido. Los postes metálicos prefabricados se fijaron con un cemento de resina dual (RelyX ARC) y los especímenes se sometieron a una prueba de extracción. El análisis estadístico se realizó mediante Shapiro-Wilk, análisis de varianza (ANOVA de una vía) y pruebas de Tukey-Kramer (p<0,05). Resultados: Los grupos rellenos con Endofill (GI, GII, GIII, GIV) presentaron los valores más bajos de fuerza de unión, los cuales fueron similares entre sí (p>0,05). Los mayores valores de fuerza de unión se observaron en raíces rellenas con AH Plus (GV, GVI, GVII, GVIII), principalmente sin limpieza de las áreas debilitadas, seguido de grabado ácido (GVII), y también con limpieza de las áreas debilitadas aunque sin grabado ácido (GVI) (p<0.05). Conclusión: Los mayores valores de fuerza de unión se observaron en las raíces rellenas con AH Plus; (1) sin limpieza de las áreas debilitadas y con grabado ácido, y; (2) con limpieza de las áreas debilitadas, pero sin grabado ácido.

Late Onset Subacute Profound Biotinidase Deficiency Caused by a Novel Homozygous Variant c.466-3T>G in the BTD Gene

Biotinidase defciency (BD) is an autosomal recessive disorder caused by bi-allelic mutation in the BTD gene. Clinical manifestations in BD mainly depends on residual biotinidase enzyme activity, although there are some exceptions. Broadly BD disorders are classifed as profound BD and partial BD. Further profound BD can be early onset, late onset, and sometimes may be asymptomatic. Clinically late-onset profound BD can present with spectrum of manifestations ranging from single organ to multiple organ involvement, typically afecting function of brain, eye, ear, and skin. Here, a frst-born child to consanguineous parents with late-onset profound BD presenting with hyperventilation secondary to lactic acidosis, hypotonia, evolving spasticity, and abnormal neuroimaging fndings caused by novel homozygous variant, c.466-3T>G in the BTD gene is reported.

Treatment of Insomnia from the Biased Constitution of the Elderly / 浙江中医药大学学报

[Objective] This article elaborated the clinical experience of Professor HAN Xu in the treatment of insomnia by using the theory of biased constitution. [Methods]For the sake of elaborating Professor HAN's experience in the treatment of insomnia, the author summed up the characteristics of old people's constitution and explained the relationship between the two from excess and deficiency syndrome. In order to better describe Professor HAN's experience in insomnia, the author enumerated 7 classic cases from several different aspects, like etiology and pathogenesis, therapy and prescriptions.[Results] Professor HAN believes that the characteristics of insomnia include the two aspects of deficiency and excess. The deficiency constitution should distinguish the deficiency between Qi and blood, Yin and Yang. The ways to treat the disease are invigorating Qi and nourishing blood, warming Yang and dispersing the cold, nourishing Yin and latenting Yang, striking the fire down. The Chinese medicinal formulae to treat the disease may use Decoction for invigorating the spleen, Mahuang Fuzi Xixin Tang, Fire diversion soup, Qianyang Fengsui Dan. The excess constitution should pay attention to phlegmatic hygrosis, stagnant blood, Qi-depression, fire -evil. The ways to treat the disease are soothing liver and invigorating spleen, descending and eliminating phlegm, stimulating circulation to end stasis. The ways include Peipi Shugan Tang, dissipating phlegm, Xuefu Zhuyu Decoction. To distinguish the actual situation of their specimens to take methods of strengthening the body resistance and eliminating evil, clinically has good effects. [Conclusion] Professor HAN makes the program of deficiency and excess and divides the biased constitution of the aged into six different types, like the deficiency of Qi and blood, Yang deficiency and Yin excess, hyperactivity of deficient fire, Liver Qi stagnation, phlegmatic hygrosis, blood stasis. The theory shows promising clinical outcome and is worth of a wide application.

Evaluation of Stress Distribution in Endodontically Weakened Teeth Restored with Different Crown Materials: 3D-FEA Analysis

Abstract This study evaluated the stress distribution in endodontically treated teeth, weakened (W) or not weakened (NW), restored with different materials of prosthetic crown using 3D-FEA. Models of a maxillary canine were constructed based on micro-CT images and divided into the groups: G1 (control) - sound tooth; G2 to G7 - endodontically treated teeth restored with glass fiber post (GFP); which G2 to G4 simulated NW root and G5 to G7 simulated W root. For crown material the teeth were restored with: G2 and G5: metallic coping and ceramic veneering, G3 and G6: zirconia coping and ceramic veneering, G4 and G7: alumina coping and ceramic veneering. Load of 180 N was applied at the incisal third of lingual surface at 45º. Models were supported by the periodontal ligament (x=y=z=0). The von Mises stress (VMS) values were calculated. The W teeth presented higher VMS at coping when compared to NW teeth and group G1 showed lower VMS value. For crown material, for both W or NW teeth, increasing VMS was found at metallic, zirconia and alumina coping, respectively. Metallic coping showed a better performance despite its unfavorable esthetics, suggesting as an appropriate material for prosthetic restoration of endodontically treated teeth.

Resumo Este estudo avaliou a distribuição de tensão em dentes tratados endodonticamente, fragilizados (F) ou não fragilizados (NF), restaurados com diferentes materiais para a coroa protética utilizando 3D-FEA. Modelos de um canino maxilar foram construídos baseados em imagens de micro-CT e divididos em grupos: G1 (controle) - dente hígido; G2 a G7 - dentes tratados endodonticamente com pino de fibra de vidro (PFV), sendo que G2 a G4 simularam raízes NF e G5 a G7 simularam raízes F. Para o material das coroas os dentes foram restaurados com: G2 e G5: coping metálico e revestimento cerâmico, G3 e G6: coping de zirconia e revestimento cerâmico, G4 e G7: coping de alumina e revestimento cerâmico. Carregamento de 180 N foi aplicado na superfície lingual em seu terço incisal com 45 graus de inclinação. Os modelos foram suportados pelo ligamento periodontal (x=y=z=0). Os valores da tensão de von Mises (VMS) foram calculados. Os dentes F apresentaram maiores valores VMS para o coping quando comparados aos dentes NF, sendo que o G1 apresentou menores valores VMS. Para o material das coroas, ambos F ou NF aumentaram VMS no coping metálico, zirconia e alumina, respectivamente. Copings metálicos apresentaram melhor comportamento mecânico apesar de não favorecerem a estética, o que sugere ser um material apropriado para a restauração de dentes tratados endodonticamente.

Finite element stress analysis of weakened root of maxillary central incisors restored with titanium post-core and fiber post-core systems / 西安交通大学学报(医学版)

Objective To analyze the stress distribution of the weakened root dentine restored with titanium post-core crown and fiber post-core crown by finite element method.Methods The sample was scanned by Inveon Micro-CT technology from incised margin to root apex at 21 μm interval to obtain transverse data of DICOM format.The three-dimensional finite element model was created by rebuilding the morphology of maxillary central incisor with Inveon Research Workplace as well as Mimics,Geomagic and Catia software.The model was meshed and loaded to analyze the stress distribution on the weakened dentine of root by finite element method.Results The maximum stress,shearing stress and equivalent stress of the root restored with fiber post-core crown were 60%less than the stresses of the root restored with titanium post-core crown in the experiment.The stress on various parts of the root restored with titanium post-core crown was higher than that of the root restored with fiber post-core crown.Furthermore,the stress concentration occurred mainly on the labial interface of the upper 1/3 of root. Especially when the root was restored with titanium post-core crown,the stress value reached 37 .73 MPa,which was 1.9 times that of fiber post-core crown.Conclusion The finite element analysis indicates that the fiber post-core crown is better than titanium post-core crown in avoiding stress concentration and root fracture in the restoration of weakened root of maxillary central incisors.

Update on effect of ferrule on teeth restored with fiber posts / 口腔疾病防治

@#Among the variety of post and core materials, fiber post is getting more and more popular because of its excellent physical and mechanical characters, superior aesthetics properties. However, it also has some limitations. In the restoration of damaged teeth, there should be more dentin ferrule, and the different designs of ferrule affect the retention, stability and root fracture resistance of teeth restored with fiber posts. The purpose of this article is to give a literature review of the effect of ferrule on teeth restored with fiber posts.

Incapacidade funcional: condições de saúde e prática de atividade física em idosos / Discapacidad funcional: condiciones de salud y práctica de actividad física en ancianos / Functional disability: health conditions and physical activity practice in older adults

RESUMO Objetivo: verificar a prevalência de incapacidade funcional entre idosos e sua associação com condições de saúde e prática de atividade física regular. Método: trata-se de um estudo de base domiciliar, transversal, realizado com idosos de ambos os sexos. As variáveis associadas à incapacidade funcional foram verificadas por meio de regressão de Poisson. Resultados: participaram deste estudo 420 idosos (68,1% mulheres). Observou-se associação estatisticamente significativa entre incapacidade funcional e número de doenças crônicas, autoavaliação de saúde e prática de atividade física, essa última apenas entre os homens. Idosos que referiram quatro ou mais doenças crônicas, que autoavaliaram a saúde como ruim e que não praticavam atividade física, apresentaram elevadas prevalências de incapacidade funcional. Conclusão: considerando o caráter modificável dessas variáveis, recomendam-se ações de prevenção, principalmente em nível primário, que retardem o surgimento de incapacidades.

RESUMEN Objetivo: verificar la prevalencia de discapacidad funcional entre ancianos y su asociación a condiciones de salud y práctica regular de actividad física. Método: estudio de base domiciliaria, transversal, realizado con ancianos de ambos sexos. Las variables asociadas a la discapacidad funcional fueron verificadas mediante regresión de Poisson. Resultados: participaron del estudio 420 ancianos (68,1% mujeres). Se observó asociación estadísticamente significativa entre discapacidad funcional y número de enfermedades crónicas, autoevaluación de salud y práctica de actividad física, esta última sólo entre los hombres. Los ancianos que informaron cuatro o más enfermedades crónicas, que autoevaluaron su salud como mala y que no practicaban actividades físicas presentaron prevalencias elevadas de discapacidad funcional. Conclusión: considerando el carácter modificable de estas variables, se recomiendan acciones de prevención, particularmente a nivel primario, con el fin de retardar el surgimiento de discapacidades.

ABSTRACT Objective: to verify the prevalence of functional disability among older adults and how it can affect their health conditions and the regular practice of physical activities. Method: this is a household and cross-sectional study conducted with older adults of both sexes. We verified the variables associated with functional disability by Poisson's regression. Results: around 420 older adults participated in this study (68.1% of them being women). We observed a statistically significant association between functional disability, the number of chronic diseases, self-assessed health conditions, and the practice of physical activities; the latter only being found among men. Older adults who reported presenting four or more chronic diseases, self-assessed their health conditions as poor, and were not used to practice physical activities, showing high prevalence of functional disability. Conclusion: considering the changeable character of these variables, we recommend that prevention actions be taken, mainly at primary level, to delay the emergence of disability.

Effects of Different Materials Post-core Restoration on Fracture Resistance of Weakened Roots / 中国医科大学学报

Objective To investigate the influence of different kinds of post?core materials on fracture resistant ability of different walls during weakened root restoration. Methods Forty?eight human single root mandibular first premolars were randomly assigned to two groups(groupⅠwas repaired with quartz?fiber post?core and mental crown ,and groupⅡwas repaired with Co?Cr cast metal post?core and mental crown). Each main group was then divided into four subgroups of 6 specimens. All samples were tested on the universal test machine. We observed the fracture load and the fracture mode of the specimens. Results Cr?Co ally post?core group exhibited significantly higher resistance to fracture than quartz fiber post?core in the same number weakened root walls(P<0.05). With the increase in the quantity of weakened root walls ,the fracture resistance was significant decreased in the same post?core material(P<0.05). The possibility of repairable fractures in groupⅠwas significantly higher than that in groupⅡ(P<0.05). Conclusion Cr?Co alloy post?core can bear a higher bite ,but quartz fiber post?core has the most favorable fracture modes.

Effect of Light Sources on the Bond Strength of Resin Material to Thin-walled Roots

The aim of this study was to evaluate the bond strength to the dentin of an adhesive material used for root reinforcement light activated with different sources. Roots were divided into 4 groups (n=15) according to the light source used to activate the resin reinforcement: GI, non-weakened roots (control); GII, halogen light (H) 600 mW/cm2; GIII, LED 800 mW/cm2 and GIV, LED 1500 mW/cm2. The reinforcement was done with adhesive, composite resin and fiberglass posts. After 24 h, the specimens were sectioned and the first slice of each post region was used in the push out test in a universal testing machine with a crosshead speed of 0.5 mm/min. Failure modes of the debonded specimens were examined. Data (MPa) were analyzed by ANOVA and Holm-Sidak test (=0.05). The second slice from each region was analyzed by scanning electron microscopy (SEM). LED-1500 (4.69 ± 1.74) provided bond strength similar to the control group (5.05 ± 2.63) and statistically different from H-600 (1.96 ± 0.94) and LED-800 (2.75 ± 1.90), which were similar to each other (p<0.05). Cervical (4.16 ± 2.32) and middle (4.43 ± 2.32) regions showed higher bond strength than the apical (2.25 ± 1.50) (p<0.05). There was a prevalence of adhesive failures in H-600 and LED-800 and cohesive failures in LED-1500. SEM showed the formation of long, numerous and fine tags. It was concluded that LED-1500 provided higher bond strength of resin reinforcement to the dentin.

O objetivo deste estudo foi avaliar a resistência de união à dentina de uma resina utilizada para reforço de raiz, ativada com diferentes fontes de luz. De acordo com a fonte de luz utilizada as raízes foram divididas em 4 grupos (n = 15): GI, raízes não fragilizadas (controle); GII, luz halógena (H) 600 mW/cm2; GIII, LED 800 mW/cm2 e GIV, LED 1500 mW/cm2. O reforço foi feito com adesivo, resina composta e pino de fibra de vidro. Após 24 h, os espécimes foram seccionados e o primeiro slice de cada região utilizado para o teste de união push out, na máquina de ensaios universais com 0,5 mm/min, e o tipo da falha avaliada. Os dados obtidos (MPa) foram analisados utilizando os testes de ANOVA e Holm-Sidak (=0.05). O segundo slice de cada região foi analisado por microscopia eletrônica de varredura (MEV). O LED-1500 (4.69 ± 1.74) proporcionou resistência a união similar ao controle (5.05 ± 2.63) e estatisticamente diferente do H-600 (1.96 ± 0.94) e LED-800 (2.75 ± 1.90), que são similares entre si (p<0.05). As regiões cervical (4.16 ± 2.32) e média (4.43 ± 2.32) apresentaram alta resistência à união quando comparadas à região apical (2.25 ± 1.50) (p<0.05). Houve uma predominância de falhas adesivas com as fontes de luzes H-600 e LED-800 e coesivas com o LED-1500. A análise em MEV demonstrou a formação de longos tags resinosos. Desta forma, pode-se concluir que o LED-1500 proporcionou maior resistência à resina utilizada para o reforço da dentina radicular.

An In Vitro study of Fracture Resistance of Weakened Tooth Roots Reinforced with Two Types of Adhesive Restorative Materials.

Introduction: Composite resin (CR) is among the commonly used material for intraradicular reinforcement of weakened tooth roots. Purpose: This study was to compare the fracture resistance of experimentally weakened tooth roots reinforced using auto-polymerized composite resin and light-polymerized composite resin. Materials and Methods: Fifty-six extracted human maxillary incisors were divided into 2 groups (n = 28) and the root canals were over prepared to weaken it. The samples in Group A were restored using light-cured CR Z100 and light-transmitting polymerizing post (Luminex), whereas Group B using auto-cured CR Alpha-dent. Both groups were placed with metal parapost cemented with a resin luting cement (Nexus 2). Specimens were subjected to compressive load (N) using Instron machine until fracture. Data were submitted to independent t test analysis of variance (p < 0.05). Results: There was no significant difference (p = 0.233) in fracture resistance between the teeth reinforced with light-polymerizing and auto-polymerizing CR are 549.3 (± 95.44) and 490.7 (± 110.37) respectively. Conclusion: The use of less technique sensitive auto-polymerizing CR give equivalent benefit effect on reinforcing weakened roots, as the more commonly light-polymerized composite resin.