RESUMO
Objective: To optimize the extraction process parameters of Guizhi Shaoyao Zhimu Granules (GSZG). Methods: Using high performance liquid chromatography and Elisa, the extraction rate of trans-cinnamaldehyde, paeoniflorin, mangiferin, glycyrrhizic acid, the dry extract yield of extracted herbs and inflammatory factor IL-6 were comprehensively evaluated. The information entropy weighting method was used to determine the objective weight of each index, and response surface methodology was adopted to optimize the extraction process parameters of GSZG. Results: The method had good linear relationship within the range of 3.27-104.64 μg/mL for trans-cinnamaldehyde (r = 0.999 9), 8.16-261.03 μg/mL for paeoniflorin (r = 1.000 0), 1.39-89.27 μg/mL for mangiferin (r = 0.999 9), and 1.00-33.00 μg/mL for glycyrrhizic acid ammonium salt (r = 0.999 9). Guizhi-Shaoyao-Zhimu extraction had the effect of inhibiting the proliferation of MH7A cells (molded by TNF-α) with IC50 of 1.02 mg/mL, and had anti-inflammatory activity. Entropy method was more scientific, reasonable and stable. According to the comprehensive scoring results and practical situation, it was determined that the best extraction process of the preparation was to add 16 times the amount of water, and decocted three times for 1 h each time. The RSD of the predicted value was less than 3% compared with the measured value. Conclusion: The preferred process has high extraction rate, with good stability and repeatability, which is suitable for the mass production of GSZG.
RESUMO
Objective: To provide a reference for the industrial production of the extraction process of Compound Baihuang Xiere Zhili Tablets (CBXZT) by exploring the optional extraction process parameters based on determining the index weight. Methods: With the extraction capacity of anemoside B4, berberine hydrochloride, aesculin, aesculetin, scopoletin, and ratio of extraction as comprehensive evaluation indexes, the weighting coefficient in six synthetic evaluation indexes was determined by Analytic Hierarchy Process (AHP), Criteria importance through intercriteria correlation (CRITIC) method, and mixed weighted AHP-CRITIC. And ethanol-extracting process was optimized by orthogonal test. Results: The excellent linearity with correlation coefficients were 121.1-1211.0, 82.2-822.0, 22.3-223.0, 15.42-154.2, and 1.6-16.0 μg/mL, r of 0.9997-0.9999 was obtained, and the average recoveries of the five compounds were 99.23%-100.63% with all RSD values less than 3.0%. Mixed weighted AHP-CRITIC was more scientific, reasonable, and stable than AHP and CRITIC. The optimal process parameters were confirmed to be decocting for thrice with 2 h in each by eight times the amount of 60% ethanol, according to the weight coefficient determined by comprehensive evaluation. Conclusion: These optimized technologies are stable, feasible, and suitable for standardized production of CBXZT. The weight coefficient determined by mixed weighted AHP-CRITIC was objective, real and could reflect the compound compatibility information. These optimized technologies are stable, feasible and suitable for standardized production of CBXZT.
RESUMO
Objective To evaluate the effect of midwives′antenatal clinic service on management of gestational weight and birth outcomes .Methods Totally 420 primiparas who had made registration and regular antenatal examination in a hospital ,were randomly divided into two groups .The women in the control group(n=208) received routine antenatal clinicservice .In addition ,the women in the experimental group(n=212) participated in an individual midwives′antenatal clinic .The primiparas′gestational weight gain and birth outcomes were measured and compared between the two groups .Results The rate of natural birth in the experimen‐tal group was higher than that of the control group .The rate of fetal macrosomia ,episiotomy and labor analgesia were lower than that of the control group(P0 .05) .Conclusion The midwives′antenatal clinic service has good effects on management ofgestational weight gain ,improves birth outcomes and pro‐vides primiparas positive experience of childbirth .