Content determination of alkaloids in different parts of Papaver nudicaule from different populations / 中草药

Objective To determine the qualitities Papaver nudicaule from different populations, a total alkaloid and three main kinds of alkaloids (reframidine, nudicauline, and amurensinine) from different parts, in order to provide a scientific basis for rational utilization and quality evaluation of P. nudicaule. Methods The qualitative and quantitative analysis of P. nudicaule has been conducted based on the method of LCMS-IT-TOF, Bromocresol green acid dye colorimetry and UPLC-MS, respectively. Results From the quantitative analysis, 13 kinds of alkaloids have been analyzed, in which eight kinds of chemical structures have been identified, and the results of quantitative analysis showed that there were greater variabilities in total alkaloid and three main kinds of alkaloids. Comprehensively, the plants grown in WLBT, HGL, and DL were better than other populations, in which the plants grown in WLBT were the best. In addition, the contents of total alkaloid and three main kinds of alkaloids were highly existed in the entire plants and flowers. Conclusion Based on the evaluation of the quality characteristics of P. nudicaule, it is suggested that total alkaloid and nudicauline can be seen as main reference indexes. Additionally, the result suggests that WLBT, HGL, and DL can be chosen to select and breed excellent quality of P. nudicaule, develop the quality standard and make layout of source area. The best harvest time should be chosen in late July to early August, and harvest site should be chosen flowers and whole plants.

Larvicidal Activity of Tephrosia purpurea, (L) Against the Larvae of Culex quinquefasiciatus.

The aim of this work was to study the larvicidal activity of Tephrosia purpurea (L) Pers. against the larvae of culex quinquefasiciatus. The preliminary laboratory trail was undertaken to determine the efficacy of petroleum ether and ethyl acetate extract of dried whole plant of Tephrosia purpurea belonging to the family Papilionaceae at various concentrations against the late third or early fourth instar larvae of culex quinquefasiciatus by following the WHO guidelines. The results suggest that 100% mortality. Petroleum ether and ethyl acetate extract of Tephrosia purpurea (L) Pers. was observed at 250ppm and 300ppm respectively. The results suggested that use of plants in insect control as an alternative method for minimizing the noxious effect of some pesticide compound on the environment. Thus the extract of Tephrosia purpurea delivers promising more selective and biodegradable agent.