RESUMO
Objective To investigate whether emodin(1,3,8-trihydroxy-6-methylanthraquinone) can attenuate inflammatory response in rats' lungs with acute necrotic pancreatitis (ANP). Method Acute necrotic pancreatitis model was induced by injection of 3% sodium taurocholic acid into the subcapsular of pancreas and emodin was administered by intestine perfusion. Plasma amylase of 3, 6 and 12 h with acute necrotic pancreatitis was measured together with the detection of IL-1β, IL-6 and IL-10 mRNA expressions in rats' lungs by semi-quantitative RT-PCR. Immunohistochemistry was detected the expression of IL-1β converting enzyme (ICE) in the rats' lungs. Result Plasma amylase of 3, 6 and 12 h with acute necrotic pancreatitis groups are obviously high as compared with normal group(P＜0.05). Plasma amylase was (1 611.20±218.72)IU/L in normal group. Plasma amylase of 3, 6 and 12 h with acute necrotic pancreatitis groups were (1 981.40±56.81)IU/L, (3 287.40±612.37)IU/L and (4 914.60±746.82)IU/L. Plasma amylase of 3, 6 and 12 h with acute necrotic pancreatitis after treatment with emodin groups were obviously low as compared with acute necrotic pancreatitis groups. The plasma amylase was(1 617.20±136.80)IU/L,(2 323.40±318.19) IU/L and (2 670.20±390.03)IU/L respectively. The study showed that the mRNA expression of pro-inflammatory cytokin IL-1β and the expression of IL-6, as well as the expression of IL-1β converting enzyme(ICE) were decreased and IL-10 was increased. Conclusion The study demonstrates that emodin plays an important role in reducing plasma amylase level. Emodin exerts anti-inflammatory effects in acute necrotic pancreatitis rats' lungs by downregulating the mRNA expression of IL-1β and IL-6 and upregulating the mRNA expression of IL-10.
RESUMO
Study on 70 acute pancreatitis patients (40 males, 30 females, aged 19 - 76) operated in Viet Duc Hospital from January 1999 to May 2002. Exclusive criteria: edema acute pancreatitis, necrotic pancreatitis without operation, necrotic pancreatitis was operated in basic settings. The results: the most of necrotic pancreatitis had no clear causes (74.28%). Mechanic causes included gallstone, worm (17.15%), acute pancreatitis after gall-pancreas area operation (1.43%). The disease was common in adults, mainly in 41-60 years old. In necrotic pancreatitis, pancreas could be necrotic very fast during the first 24 hours with shock, multi-organs failure, tympanites, vomit, peritoneum reaction and induction signs. The common symptoms were : increased blood amylase and urine amylase, leukocyte, blood urea, blood glucose; decreased blood calcium. CT scanner with a high accuracy rate (100%), was a gold criterion in diagnosis of necrotic pancreatitis. In operation, should minimized intervention. It is necessary to perform jejunotomy to decrease the expense due to intravenous feeding regularly.
Assuntos
Pancreatite , TerapêuticaRESUMO
OBJECTIVE:To observe the pharmacological effect of Ulinastatin on acute necrotizing pancreatitis in rats.METHODS:Animal models were divided into3groups:group A,sham operation;group B,acute necrotic pancreatitis given no treatment;group C,acute necrotic pancreatitis treated with Ulinastatin.The changes of AMS and TNF-?were compared at different time among3groups.RESULTS:AMS and TNF-?in group C were significantly different from those in group A and in group B.CONCLUSION:Ulinastatin could remarkably improve the prognosis of acute necrotic pancreatitis.