Cytotoxic effect and mechanism of Aflatoxins B1 on Vero E6 cells / 国际检验医学杂志

Objective To explore the cytotoxic effects and mechanisms of Aflatoxins B1(AFB1) on the African green monkey kidney cells (Vero E6).Methods After incubation of Vero E6 cells with AFB1,cellular toxicity was assessed by CCK-8 assay and LDH release rate.Annexin V/PI double staining and DAPI staining was used to detected cell apoptosis.Results AFB1 has significant toxic effects on Vero E6 cells.Cell viability was less than 10% when treated by 200 μmol/L AFB1 for 48 h.LDH release rate was increased significantly with the increase of AFB1 concentration.Externalization of cell membrane phosphatidylserine was observed by Annexin V/PI double staining.Nucleus fragmentation was detected by DAPI staining.Conclusion AFB1 exhibits significantly cytotoxic effects on Vero E6 cells by inducing cell apoptosis.

Separação e quantificação das aflatoxinas B1, B2, G1 e G2 por cromatografia líquida de alta resolução / Separation and quantification of aflatoxins B1, B2, G1, G2 by high-performance liquid chromatography

É descrito um método para determinação das aflatoxinas B1 B2 G1 e G2 por cromatografia líquida de alta resolução. As aflatoxinas foram extraídas, cromatografadas em coluna de fase reversa, e detectadas no ultravioleta a 350 nm. Nos testes efetuados, a recuperação variou de 92 a 106%. ° método foi aplicado em dez amostras de farelo de amendoim e em amendoim em grão. °limite de detecção foi de 2 ppb (/Lg/kg) (AU).