RESUMO
BACKGROUND AND OBJECTIVES: Aloe is one of the well known substances to decrease inflammation and promote wound healing. Although little is known about its mechanism, recent studies suggest that aloe vera extracts may have the anti-allergic effect by inhibiting the mediator release evoked by mast cell activation. Therefore, we were to investigate the effect of NY945, a glycoprotein fraction of Aloe vera, on nasal mucosa of the ovalbumin (OVA) sensitized guinea pigs. Materials and Methods: Female Dunkin-Hartley guinea pigs were sensitized with intraperitoneal injection and subsequent inhalation of OVA. NY945 was injected intraperitonealy before intranasal challenge of OVA with an ultrasonic nebulizer. Airway resistance, mucosal permeability, histamine concentration in the nasal lavage fluid, the number of mast cells and eosinophils in nasal mucosa and serum level of OVA -specific IgE were evaluated. RESULTS: NY945 effectively inhibited respiratory airway resistance in the sensitized guinea pigs. The concentration of dye into nasal lavage fluid was rapidly increased after OVA instillation and NY945 inhibited the OVA -induced microvascular dye leakage. OVA inhalation to sensitized guinea pig resulted in prominent increase of histamine concentration in the nasal lavage fluid. This response was inhibited by intraperitoneal NY945 injection. It also inhibited accumulation of eosinophils in the nasal mucosa in sensitized guinea pigs. However, there was no change in the serum OVA -specific IgE level between the NY945 treated and control group. CONCLUSION: The data suggest that NY945 inhibits early allergic reaction via inhibition of histamine release and eosinophil infiltration.
Assuntos
Animais , Feminino , Humanos , Resistência das Vias Respiratórias , Aloe , Eosinófilos , Glicoproteínas , Cobaias , Guiné , Histamina , Liberação de Histamina , Hipersensibilidade , Imunoglobulina E , Inflamação , Inalação , Injeções Intraperitoneais , Mastócitos , Líquido da Lavagem Nasal , Mucosa Nasal , Nebulizadores e Vaporizadores , Ovalbumina , Óvulo , Permeabilidade , Ultrassom , CicatrizaçãoRESUMO
Objective: To study the pharmacological effects of Radix Saposhnikoviae (RL) and Fruetus Tribuli (FI) used singly and combinedly. Methods: The effect of the tested drugs on the animal itching models induced by dextran 40 and Histanmine, skin capillary permeability induced by Histamine and experimental nettle rash induced by dimethylsulfoxide (DMSO) were observed. Results: RL and FI used singly and combinedly could obviously relieve the itch, inhibit the increase of skin capillary permeability induced by Histamine and resist experimental nettle rash induced by dimethylsulfoxide (DMSO) in different degree. Conclusion:RL and FI used singly and combinedly have anti allergic effect and the effect of RL and FI used combinedly is the same as used respectively.