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Objective To study the effects of chrysophanol(Chry)on NO of brain tissue and anti-anoxia in mice with cerebral ischemia-reperfusion injury. Methods A total of 75 SPF Kunming mice were randomly allocated into five groups:sham operation group, ischemia-reperfusion group, high-dose group (Chry 10.0 mg ·kg-1), medium-dose group (Chry 1.0 mg ·kg-1) and low-dose group (Chry 0.1 mg ·kg-1). Using improved Himori method, cerebral ischemia reperfusion-injury model was produced in conscious mice by temporarily obstructing bilateral common carotid arteries. The neurological function was measured according to the Bederson scoring standard. The mice were subjected to decapitation for hypoxia tolerance test. The gasping time was measured by anoxia tolerance test in beheaded mice. The level of NO in cerebrum was detected. Results Chrysophanol can decrease the level of NO in cerebrum of mice with cerebral ischemia-reperfusion injury and prolong the gasping time in beheaded mice with cerebral ischemia-reperfusion injury [low-dose group, (14.6±1.2) s; medium-dose group, (16.4 ± 1.2)s; high-dose group, (17.4 ± 1.1)s; ischemia-reperfusion group, (13.2 ± 1.0)s, P<0.05]. Conclusion The protective effects of chrysophanol on cerebral ischemia-reperfusion injury are involved in decreasing the content of NO in brain tissue and anti-anoxia in mice.
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Objective To investigate the effects of Ento-I plastic on anoxia and myocardial ischemia. Methods The effect of Ento-I plastic on anoxia in mice was evaluated by testing mice’s anti-anoxia time under ordinary pressure, mice’s gasp time and the number of mouth breathing under ischemia-anoxia, and mice’s survival time after sodium nitrite poisoning. Animals were divided into normal saline(NS)group, matrix group, aspirin(ASP)group and Ento-I plastic(9.125, 18.25 and 37.5 mg/kg)groups. The effect of Ento-oon myocardial ischemia in mice was preliminarily evaluated using the animal model of acute myocardial ischemia induced by the subcutaneous injection of isoprenaline(the animals were divided into NS group, matrix group, compound danshen dropping pill(DSP)group and Ento-I plastic(5, 10 and 20 mg/kg)groups. In these experiments, NS group, ASP group and DSP group were administered intragastrically, while the matrix group and Ento-I plastic groups were administered by daubing on both sides of the temple. Results There was no significant difference in all indexes between the NS group and the matrix group in all four experiments. Compared with NS, the middle dosage of Ento-I plastic(18.25 mg/kg)could obviously prolong the anoxia tolerance time to (50.87±11.90)min under ordinary pressure, survival time to(14.15±4.61)min after sodium nitrite poisoning and the gasp time to (27.90±4.79)s after decapitation, with the statistically significant difference(P<0.01). In anti-myocardial ischemia experiment, both the myocardial superoxide dismutase(SOD)activity and malondialdehyde(MDA)content were significantly decreased in the high dosage Ento-I(20 mg/kg)and DSP groups than in the NS group(P<0.01). The pathological examination of the ischemic of myocardium itself showed that Ento-I plastic could significantly reduce ischemic myocardial injury and enhance the anti-ischemic effect of myocardium itself. Conclusion The Ento-I plastic could obviously strengthen the anti-anoxic and anti-ischemic ability of mice.
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Aim To observe the effects of active com-ponent of Radix Isatidis ( ACRI ) on anti-anoxia and anti-fatigue in mice and investigate its possible mecha-nism. Methods Based on the weights, the mice were randomly divided into 5 groups: blank control group, ACRI 25, 50 and 100 mg·kg-1 groups, positive drug ( American ginseng liquid) control group 3 mL·kg-1 . Drugs were administered to the mice for about 14 con-secutive days, and during the experiment general situa-tions of mice were observed. The experiment of bearing hypoxia at normal pressure and the experiment of swim-ming while weight-bearing were conducted to study the effect of ACRI on anti-anoxia and anti-fatigue in mice. Then the superoxide dismutase ( SOD ) activities, the content of maleic dialdehyde ( MDA ) of mice serum and liver and blood urea nitrogen, blood lactic acid, liver glycogen were detected, in order to investigate its mechanism. Results ACRI decreased the growth rate of body weight in mice significantly, obviously pro-longed the survival time of anoxic mice at normal pres-sure and the swimming time of loaded mice, enhanced the SOD activities of mice blood and liver, decreased the MDA content of mice blood and liver, increased the content of liver glycogen, and decreased the blood urea nitrogen and blood lactic acid in mice after swim-ming. Conclusion ACRI has the anti-anoxia and anti-fatigue functions.