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Objective:To construct a "drug-target-pathway" network of Polygalae Radix and Gastrodiae Rhizoma in treating epilepsy, in order to explore the "multi-component, multi-target, multi-pathway" mechanism of the two kinds of traditional Chinese medicines. Method:According to the reverse pharmacophore matching method, potential targets of Polygalae Radix and Gastrodiae Rhizoma were predicted. Biological information annotation databases were used to analyze the molecular function and biological process of the action targets. Cytoscape 3.2.1 software was used to construct the "ingredient-core, target-pathway" network of Polygalae Radix and Gastrodiae Rhizoma for the treatment of epilepsy. Result:The 14 major constituents from Polygalae Radix exhibited interactions with 105 potential targets, and the 12 compounds from Gastrodiae Rhizoma showed interactions with 109 potential targets, involving several cancer signaling pathways, neuroactive ligand-receptor interaction and mitogen-activated protein kinase(MAPK) biological process played roles in the treatment of epilepsy. Conclusion:According to the screening for the potential targets relating to epilepsy and the evidences obtaining from docking study, we demonstrated that constituents from Polygalae Radix and Gastrodiae Rhizoma could play an anticonvulsant role by mediating the levels of monoamine substances. The conclusion is close to literatures published online to a certain degree, suggesting the accuracy of the study on the effect of Polygalae Radix and Gastrodiae Rhizoma in treating epilepsy according to the network pharmacology.
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Objective: To establish a screening model in vitro with γ-aminobutyric acid transaminase (GABA-T) as a target for screening the inhibitors of anti-epilepsy drugs for the treatment of nervous diseases and finish the structure-activity relationship analysis of p-hydroxybenzaldehyde as effective components in Gastrodiae Rhizoma and its analogs. Methods: Catalyst performed temperature, reaction time, the concentration of NAD+, α-ketoglutarate, and GABA were investigated to optimize the model. The test of p-hydroxybenzaldehyde (HBA) and its 11 analogs were selected as verification of this model. Results: The screening model in vitro based on GABA-T was established. The testing result of p-HBA and its 11 analogs was consistent with the practical activity recorded in the literature. Hydroxyl groups of -OH and -CHO in p-aldehyde on the benzene ring were pharmacophores. Conclusion: The model established in this paper can be used for high throughput screening the inhibitors of anti-epilepsy drugs, which provides the reference for the study on the inhibitors of anti-epilepsy drugs for nervous diseases and their mechanisam.
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El dolor neuropático surge como consecuencia directa de una lesión o enfermedad que afecta al sistema somatosensorial. En el niño existen numerosas causas de dolor neuropático: traumáticas, síndrome doloroso regional complejo tipo 1, enfermedades neurológicas y neuromusculares, infecciones crónicas, cáncer y causas genéticas. Su diagnóstico puede ser difícil en los niños. El tratamiento del dolor neuropático es un reto para los médicos que se dedican a su atención. Los medicamentos antidepresivos tricíclicos como la amitriptilina y la imipramina y los antiepilépticos, se emplean con frecuencia en el tratamiento del dolor neuropático en los niños. También se emplean el acetaminofén (paracetamol), medicamentos antiinflamatorios no esteroideos y el tramadol. A todo niño y adolescente con sospecha de dolor neuropático se le debe realizar una evaluación clínica, que incluya una exhaustiva anamnesis y examen físico general, regional y por aparatos, con especial énfasis en el examen neurológico. Un examen neurológico normal no excluye la presencia de dolor neuropático.
Neuropathic pain occurs as direct result of a lesion or a disease affecting the somatosensory system. The neuropathic pain in a child has a number of causes: traumas, complex regional pain syndrome type 1, neurological and neuromuscular diseases, chronic infections, cancer and genetic causes. Its diagnosis may be difficult in children, so the treatment is a challenge posed to physicians who specialized in the field. Tricyclic antidepressive drugs as amitryptiline and imipramine and antiepilepsy drugs are often used in the treatment of neuropathic pain in children. Acetaminophen (paracetamol), non-steroidal anti-inflammatory drugs and tramadol are also used. Every child and adolescent suspected of neuropathic pain should be clinically assessed including exhaustive anamnesis and general, regional physical examination and use of equipment, with special emphasis on the neurological examination. A normal neurological test does not exclude the presence of neuropathic pain.
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Humanos , Criança , Exame Físico/métodos , Dor Aguda , Dor Crônica , Ketamina/uso terapêutico , Anamnese/métodosRESUMO
Objective Study on efficacy of epileptic patients on treatment with modified Chaihu Guizhi De -coction(Chinese traditional medicine compounds )combined with anti-epilepsy drug.Methods 90 case of epileptic patients were randomly assigned to study group or control group ,45 case for each .The control group was treated only with anti-epilepsy drug , and the study group was treated with modified Chaihu Guizhi Decoction and anti -epilepsy drug.Observed and compared the treatment effect as well as the monthly average of seizure frequency of the epilepsy after treatment.Results The study group effective rate was 82.22%,which significant higher than the control group (64.44%).The study group's monthly average of seizure frequency was lower than the control group , the clinical effect between the control group and the study group were differences significantly (p<0.05).Conclusion Modified Chaihu Guizhi Decoction combined with anti -epilepsy drug can significantly reduce the seizure frequency of the epi-leptic patients in the treatment of epilepsy .It's worthy to be widely applied in clinical .
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[Objective]This study was designed to identify the risk factors related with reproductive endocrinology disorder in Chinese women of child-bearing age with epilepsy.[Methods]The clinical data of 102 women with epilepsy were collected.The patient were grouped according to seven aspects(seizure onset age,seizure type,seizure frequency,duration of epilepsy,AED type,age of start AED therapy and duration of therapy)and the contribution of these factors in development of PCOS and its components were analyzed.[Results]The incidence of hyperandrogenemia in the patients with an early onset age(≤14 years old)was higher than the ones with an onset age＞14 years old.Onset age≤14 was the risk factor of hyperandrogenemia in logistic regression analysis.The incidence of a/oligomenorrhea,polycystic ovaries,hyperandrogenemia and PCOS in the valproate-treated women were 40.63%,50.00%,15.65%,and 34.38%,respectively,which were higher than the no-therapy group and nonvalproate treated group.Valproate therapy was the risk factor of PCOS and its components.[Conclusion]Valproate therapy was the risk factor of PCOS and its components in Chinese women of child-bearing age with epilepsy.Onset age≤14 was the risk factor of hyperandrogenemia.
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[Aim] To gain the fusion protein purified GST-AEP.[Methods] The protein GST-AEP was expressed in E.Coli-DH5? as a fusion protein induced by IPTG.The protein was a kind of inclusion body.The purifying and refolding to inclusion body were optimized.The purity of GST-AEP was identified by 12% SDS-PAGE and thin-layer scanning analysis.The quantitation of the fusion protein GST-AEP was done with BCA Protein Assay.[Results] Purity of GST-AEP was higher than 90% and concentration was about 0.163?g/?l.[Conclusion] The fusion protein was highly purified and the method of fusion protein purification from the inclusion body was developed,which was the basis for further study on AEP.
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Objective To study the effects of catgut implantation at acupoint on epileptic attack behavior in experimental epilepsy rats. Methods 40 rats were randomly divided into model group, catgut implantation group, western medicine group and acupuncture group. Making the acute epilepsy model by intraperitoneal injection of penicilin in rats after pretreatment, observed the latent period and the performance of epilepsy seizures within 70 minutes after intraperitoneal injection of penicilin. Results The latent period of catgut implantation, western medicine and acupuncture groups was prolonger than model group (P 0.05). The times of epileptic attack behavior in catgut implantation group including nodding/tail-flick, beard shaking, anterior/hind limb tic, systemic tic and jump was less than the model group respectively (P
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Carbamazepine ( CBZ ) serum and salivary concentration and toxic and side reactions were studied in 60 epilepsy patients. The propriate CBZ serum concentration was l7~38?mol/L, salivary concentration was 4~13?mol/L. The toxic and side reactions were correlated with serum level, there were different toxic symptoms when CBZ levels were in therapeutic range. When CBZ serum concen tration was higher than 38~51 ?mol/L, salivary concentration was higher than 14.8~17 ?mol/L, the toxic and side reactions were significant increased in our test patients.