Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 5 de 5
Filtrar
Adicionar filtros








Intervalo de ano
1.
Journal of Zhejiang University. Science. B ; (12): 71-83, 2019.
Artigo em Inglês | WPRIM | ID: wpr-1010444

RESUMO

In this study, we evaluated the effect of the herbicide propyl 4-(2-(4,6-dimethoxypyrimidin-2-yloxy)benzylamino) benzoate (ZJ0273) on barley growth and explored the potential to trigger growth recovery through the application of branched-chain amino acids (BCAAs). Barley plants were foliar-sprayed with various concentrations of ZJ0273 (100, 500, or 1000 mg/L) at the four-leaf stage. Increasing either the herbicide concentration or measurement time after herbicide treatment significantly impaired plant morphological parameters such as plant height and biomass, and affected physiological indexes, i.e. maximal photochemical efficiency (Fv/Fm), quantum yield of photosystem II (ФPSII), net photosynthetic rate (Pn), and chlorophyll meter value (soil and plant analyzer development (SPAD)). Cellular injury of herbicide-treated plants was also evidenced by increased levels of reactive oxygen species (ROS) and antioxidative enzyme activity. Elevated levels of herbicide significantly reduced the activity of acetolactate synthase (ALS)-a key enzyme in the biosynthesis of BCAAs. In a separate experiment, growth recovery in herbicide-stressed barley plants was studied using various concentrations of BCAAs (10, 50, 100, and 200 mg/L). Increasing BCAA concentration in growth media significantly increased the biomass of herbicide-stressed barley seedlings, but had no significant effect on non-stressed plants. Further, BCAAs (100 mg/L) significantly down-regulated ROS and consequently antioxidant enzyme levels in herbicide-stressed plants. Our results showed that exogenous application of BCAAs could reverse the inhibitory effects of ZJ0273 by restoring protein biosynthesis in barley seedlings.


Assuntos
Aminoácidos de Cadeia Ramificada/administração & dosagem , Antioxidantes/metabolismo , Benzoatos/farmacologia , Biomassa , Clorofila/metabolismo , Herbicidas/farmacologia , Hordeum/metabolismo , Fotossíntese/efeitos dos fármacos , Folhas de Planta/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Plântula/metabolismo
2.
Journal of Zhejiang University. Science. B ; (12): 71-83, 2019.
Artigo em Inglês | WPRIM | ID: wpr-847073

RESUMO

In this study, we evaluated the effect of the herbicide propyl 4-(2-(4,6-dimethoxypyrimidin-2-yloxy)benzylamino) benzoate (ZJ0273) on barley growth and explored the potential to trigger growth recovery through the application of branched-chain amino acids (BCAAs). Barley plants were foliar-sprayed with various concentrations of ZJ0273 (100, 500, or 1000 mg/L) at the four-leaf stage. Increasing either the herbicide concentration or measurement time after herbicide treatment significantly impaired plant morphological parameters such as plant height and biomass, and affected physiological indexes, i.e. maximal photochemical efficiency (Fv/Fm), quantum yield of photosystem II (ФPSII), net photosynthetic rate (Pn), and chlorophyll meter value (soil and plant analyzer development (SPAD)). Cellular injury of herbicide-treated plants was also evidenced by increased levels of reactive oxygen species (ROS) and antioxidative enzyme activity. Elevated levels of herbicide significantly reduced the activity of acetolactate synthase (ALS)—a key enzyme in the biosynthesis of BCAAs. In a separate experiment, growth recovery in herbicide-stressed barley plants was studied using various concentrations of BCAAs (10, 50, 100, and 200 mg/L). Increasing BCAA concentration in growth media significantly increased the biomass of herbicide-stressed barley seedlings, but had no significant effect on non-stressed plants. Further, BCAAs (100 mg/L) significantly down-regulated ROS and consequently antioxidant enzyme levels in herbicide-stressed plants. Our results showed that exogenous application of BCAAs could reverse the inhibitory effects of ZJ0273 by restoring protein biosynthesis in barley seedlings.

3.
Chinese Journal of Natural Medicines (English Ed.) ; (6): 672-676, 2014.
Artigo em Inglês | WPRIM | ID: wpr-812217

RESUMO

AIM@#To examine the possible protective effect of Satureja khuzistanica essential oil (SKE) on antioxidant enzyme activity in alloxan-induced Type 1 diabetic rats.@*METHOD@#Thirty Sprague-Dawley male rats were divided into three groups randomly; group one as control, group two diabetic, with no treatment, and group three treatment with SKE at 500 ppm in drinking water, respectively. Diabetes was induced in the second and third groups by alloxan injection subcutaneously. After eight weeks, animals were anaesthetized. Blood samples were also collected before killing to measure antioxidant enzymes activity.@*RESULTS@#SKE significantly increased the serum level of glutathione and the serum activity of glutathione peroxidase, superoxide dismutase, and catalase in the treated group compared with the diabetic untreated group.@*CONCLUSION@#The findings showed that SKE exerts beneficial effects on the antioxidant enzymes activity in alloxan-induced Type 1 diabetic rats.


Assuntos
Animais , Masculino , Antioxidantes , Metabolismo , Farmacologia , Usos Terapêuticos , Glicemia , Metabolismo , Catalase , Metabolismo , Diabetes Mellitus Experimental , Tratamento Farmacológico , Diabetes Mellitus Tipo 1 , Tratamento Farmacológico , Glutationa , Metabolismo , Glutationa Peroxidase , Metabolismo , Óleos Voláteis , Farmacologia , Usos Terapêuticos , Estresse Oxidativo , Fitoterapia , Extratos Vegetais , Farmacologia , Usos Terapêuticos , Ratos Sprague-Dawley , Satureja , Química , Superóxido Dismutase , Metabolismo
4.
Nutrition Research and Practice ; : 308-314, 2012.
Artigo em Inglês | WPRIM | ID: wpr-72111

RESUMO

Radish (Raphanus sativus L.) is a cruciferous vegetable, and its leaves have antioxidant and anticancer properties. This study was conducted to evaluate the effects of ethyl acetate extracts from radish leaves on hypertension in 11-week-old spontaneously hypertensive rats (SHRs). The SHRs were randomly divided into 3 groups of 6 rats each on the basis of initial systolic blood pressure (SBP) and were treated with oral administration of radish leaf extract (0, 30, or 90 mg/kg body weight [bw], respectively) for 5 weeks. Six Wistar rats were used as normotensive controls. The amount of the radish leaf extract had no effect on body weight. The SBP of the SHRs showed a decreasing trend with the consumption of the radish leaf extract. In the third week, the SBP of the group fed 90 mg extract/kg bw reduced from 214 mmHg to 166 mmHg and was significantly lower than that of the normotensive and hypertensive controls. The extract did not show a significant effect on the angiotensin-converting enzyme (ACE) activity in the serum, kidney, and lung. The extract increased the concentration of NO in serum and the activities of antioxidant enzymes such as glutathione peroxidase and catalase in red blood cells (RBCs). The serum concentrations of Na+ and K+ were not significantly different between all groups. However, the fecal concentrations of Na+ and K+ increased; the fecal concentrations of Na+ and K+ for the normotensive and hypertensive controls were not different. Urinary excretion of Na+ was higher in the normotensive Wistar rats than in the SHRs, while that of K+ was not significantly different. These findings indicate that consumption of radish leaves might have had antihypertensive effects in SHRs by increasing the serum concentration of NO and fecal concentration of Na+ and enhancing antioxidant activities.


Assuntos
Animais , Ratos , Acetatos , Administração Oral , Pressão Sanguínea , Peso Corporal , Catalase , Eritrócitos , Glutationa Peroxidase , Hipertensão , Rim , Pulmão , Raphanus , Ratos Endogâmicos SHR , Ratos Wistar , Verduras
5.
The Korean Journal of Nutrition ; : 801-807, 2006.
Artigo em Coreano | WPRIM | ID: wpr-652482

RESUMO

To elucidate the effect of soy isoflavone supplementation on bone mineral density and antioxidant enzyme activity in 60 postmenopausal women residing in Iksan area were recruited. There were 31 participants in the treatment group and 29 in the control group. The treatment group consumed isoflavone extract capsules daily (which contained 90 mg of soy isoflavones) for 12 weeks. The study compared before and after isoflavone intake in the following areas: Physical examination, diet survey, bone mineral density (BMD) and antioxidant enzyme activity (superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) and total antioxidant capacity (TA)). The average age of the treatment group was 64.6 years and that of the control group was 66.5 years. There were no significant differences between the two groups in terms of height, weight and body mass index. Both groups maintained a regular diet pattern in terms of their average daily nutrient intake. There were no significant differences between the treatment group (23.3 mg) and the control group (24.0 mg) in terms of daily isoflavone intake based on diet. Isoflavone supplementation of 12 weeks did not resulted in any significant changes in BMD or parameters of antioxidant enzyme activity, implying the necessity of more intensive intervention for a substantial change. In conclusion, this study revealed that antioxidant enzyme activity holds a significant relationship with the bone mineral density in postmenopausal women and further systematic research on dose and period of isoflavone supplementation is needed to clearify the positive effect of isoflavone on BMD and/or blood antioxidant capacity in postmenopausal women.


Assuntos
Feminino , Humanos , Índice de Massa Corporal , Densidade Óssea , Cápsulas , Catalase , Dieta , Inquéritos sobre Dietas , Glutationa Peroxidase , Exame Físico
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA