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AlM:To determine the relations between the changes of vascular endothelial growth factor ( VEGF ) concentration in plasma, aqueous humor and vitreous of patients with diabetic retinopathy ( DR ) and proliferative diabetic retinopathy ( PDR) , as well as to find if the condition of metabolism control of patients with DR may affect the VEGF level. METHODS: Double antibody sandwich enzyme-linked immunosorbent assay was used to measure the levels of VEGF concentration in plasma of non-diabetic retinopathy ( NDR ) patients group, background diabetic retinopathy ( BDR ) patients group, and proliferative diabetic retinopathy ( PDR) patients group. The levels of HbAlc in plasma of patients in above three groups were measured at the same time. The VEGF concentration in aqueous humor, vitreous of PDR patients group and normal group was also measured for further comprehensive analysis. Human VEGF immunoassay Quantikine with high sensibility and quality was imported from R&D Co. LTD. , USA. RESULTS: There was an increasing tendency in the VEGF concentration in aqueous humor of PDR patients group. There was no statistically significant difference as it was compared with normal group (P>0. 05). The VEGF concentration in vitreous of PDR patients group was obviously increased, there was significant difference as it was compared with normal group (P 0. 05 ). ln normal group the VEGF concentration in plasma was the highest, but in PDR patients group the VEGF concentration in vitreous was the highest. CONCLUSlON: The significantly increased VEGF concentration in eyes, especially in vitreous of PDR patients may play an important role in development and deterioration of DR. lt was clear that VEGF played its normal biological functions mainly in plasma in normal human. ln severe DR patients, abnormal high may come from ischemic and VEGF concentration in vitreous suggested that it hypoxic retina and there was a tendency to diffuse towards the front of eyes.
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Objective@#This study compared the rates of aqueous-humor flow and trabecular outflow in eyes that had undergone YAG laser iridotomy (LI) for primary-acute-angleclosure (PAC) attack and primary-angle-closure suspect (PACS).@*Methods@#Patients who had PAC attack in one eye and narrow occludable angles (PACS) in the other eye that had undergone YAG LI were recruited. All underwent complete ophthalmologic examination including gonioscopy, ultrasonic pachymetry, A scan, and fluorophotometry to determine the rate of aqueous-humor flow. The Goldmann equation was used to compute the outflow facility using the values of aqueous flow and intraocular pressure (IOP).@*Results@#Fifty eyes of 25 patients were included, 25 of which had PAC attack and 25 were PACS. The central corneal thickness (CCT), anterior-chamber depth, and anterior-chamber volume of the 2 groups were comparable. PAC-attack eyes had significantly higher IOP (18.4 mm Hg) than the PACS (14.12 mm Hg) (p = 0.001). The mean rate of aqueous flow was 2.50 ± 0.94 µL/min and 2.89 ± 1.17 µL/min in the PAC and PACS respectively (p = 0.20). The mean aqueous-outflow facility was 0.29 ± 0.18 µL/min and 0.59 ± 0.37 µL/min respectively (p = 0.0008).@*Conclusion@#A significantly lower aqueous-outflow facility was demonstrated by fluorophotometry among eyes with PAC. Despite the anatomically open angles, they continued to have higher IOPs.