Inhibition of resvertrol on osteoblast-like trans-differentiation of vascular smooth muscle cells / 中草药

Objective: To investigate the inhibition of resvertrol (Res) on angiosteosis, and to observe the effects of Res at different concentration on osteogenic conversion of human umbilical artery vascular smooth muscle cells (hVSMCs) induced by elevated calcium (Ca2+) and phosphate (P) culture. Methods: The hVSMCs were cultured in vitro, and identified by morphology and α-smooth muscle actin (α-SMA) antibody. High calcium (2.5 mmol/L) and high phosphate (3.0 mmol/L) culture was used to induce the osteogenic differentiation of hVSMCs. The calcification was confirmed by Alizarin red staining. Cells were divided into five groups such as normal control (NC, normal concentration of Ca2+ and P), positive control (PC, high Ca2+ and high P), and three Res (5, 10, and 20 μmol/L) groups. Calcium deposition in cells was measured by Arsenazo III after 12 d. Real-time PCR and Western blotting were used to detect mRNA and protein expression of BAP, BMP2, and OPN. Results: Compared to NC group, calcium deposition in the cells dramatically increased by five times in PC group, the protein expression of α-SMA decreased, and the mRNA and protein expression of BAP, BMP-2, and OPN obviously increased (P < 0.05). Res at different concentration could reduce the cellular Ca2+. The levels of BAP, BMP2, and OPN were highest in PC group than NC and three Res groups. Furthermore, the expression of BMP2 and OPN decreased with increasing Res concentration. Conclusion: Res prevents the process of the osteogenic differentiation of hVSMCs induced by high calcium and high phosphate. The degree of osteogenic differentiation is falling with increasing Res concentration.

Diagnosis and treatment of vascular complications of external iliac arteries after kidney transplantation：a report of 6 cases / 中南大学学报(医学版)

Objective: To explore the characteristics of external iliac artery vascular complications atfer renal transplantation and the diagnosis and treatment. Methods: We reviewed the clinical data of 6 patients with of external iliac artery vascular complications atfer renal transplantation from more than 2000 renal transplantation patients in the Transplantation Center of the Third Xiangya Hospital of Central South University from 2001 to 2013, and analyzed the clinical characteristics, diagnosis and treatment. Results: hTe renal allogratf was removed in 5 of the 6 patients due to repeated external iliac arteryhemorrhage: 2 patients were replaced the external iliac artery with reversed autogenous great saphenous vein, 2 patients underwent the bilateral femoral artery bypass surgery, and 1 was repaired the external iliac artery directly. The other 1 was resected the renal allograft and the involved external iliac arteries due to fungal mass in the external iliac artery. Among the 6 patients, except 1 patient died atfer the surgery of the repair of the external iliac artery, the other 5 are all alive. Conclusion: Vascular replacement and artery bypass are effective methods for patients with external iliac artery vascular complications atfer kidney transplantation.

Inhibitory effects of chitosan on the proliferation of bovine basilar artery vascular smooth muscle cells induced by angiotensin Ⅱ / 中国海洋药物

The bovine basilar artery vascular smooth muscle cells(BAVSMC S) were isolated and cultured.The effects of chitosan and enalaprilat on the proliferation of BAVSMC S induced by angiotensin Ⅱ(AngⅡ) were evaluated with MTT method,the DNA synthesis in BAVSMC S was measured by thymidine incorporation and total protein of BAVSMC S was detected by coomassie brilliant blue method.The results showed that chitosan can inhibit the proliferation of BAVSMC S induced by AngⅡ in a time and concentration dependent manner,and depress thymidine incorporation of BAVSMC S induced by AngⅡ in a concentration dependent manner chitosan can reduce the content of protein in BAVSMC S induced by AngⅡ.These findings suggested that chitosan is capable of producing an inhibitory effect on proliferation of bovine basilar artery vascular smooth muscle cells induced by AngⅡ .

Efficacy studies of buflomedil on rabbits in vivo and vitro / 中国药理学通报

In order to provide experimental fundament and theory for clinical administration, the efficacy studies of buflomedil in vitro and vivo were conducted by using rabitt's chest artery strip and rabitts acute microcirculatory disturbance model reproducted with high molecular dextran, in which peripheral vascular dilating agent tolarzoline was acted as control agent. Experimental results showed that buflomedil of 6 ? 10-5~ 6? 10-4 mol?L-1 could inhibit con-tractions of rabbit artery strip induced by NA producing concentration-dependent, and get the curves of dose-activity shift towards right, which suggests buflomedil significantly dilates microvessels and increases blood flow velocity and capillary perfusion capacity per area (P

Inhibitory Effect of SZ-1 on the Arachidonic Acid Releases in the Cerebrovascular Endothelial and Smooth Muscle Cells / 第二军医大学学报

The release of platelet activating factor (PAF) induced 14C-arachidonic acid (14C-AA) in the bovine cerebral microvascular endothdial cells (CMEC) and arterior cerebral artery smooth muscle cells (ACASMC) and the antagonism of SZ-1 are described. The results showed that 14C-AA incorporated into the cells rapidly and PAF 0.1-20?mol/L dose-dependently stimulated the AA release significantly. It indicated that the action of PAF on the cerebrovascular system was associated with the stimulation of AA release. SZ-1 0.2-20?nol/L dose-dependently inhibited the PAF induced AA release in CMBC and ACASMC, and PAF induced aggregation of washed rabbit platelets, but did not inhibited ADP or AA induced aggregation of platelet-rich plasma(PRP), and PAF production in CMEC, indicating the specific antagonism of SZ-1 on PAF receptor.