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Objective:To establish an atomic absorption spectrometry to determine the content of eight trace elements of Yu Salvia miltiorrhiza in different growth stages，namely K，Cu，Na，Mg，Fe，Zn，Ca and Mn. Method:Micro-digestion-atomic absorption spectrometry was used to determine the trace elements in the roots of Yu S. miltiorrhiza. The HPLC method was used to determine the content of active constituents of Yu S. miltiorrhiza. Principal component analysis and correlation analysis were used to evaluate the results. Result:The contents of trace elements in Yu S. miltiorrhiza in different growth periods were significantly different. Cu had a significant positive correlation with the growth period，while Zn，Ca and Mn had significant negative correlations with the growth period. The comprehensive score of Yu S. miltiorrhiza in December was the best. The content of index components was negatively correlated with Mn，Zn and Ca，and positively correlated with Cu，Fe and Na. In soil，Mg，Fe，Ca and Mn were correlated with Zn，Ca and Mn，while Mn was negatively correlated with Cu. The content of K and Mg in the crude drug increased gradually with the change of the growth period，and the overall score of annual Yu S. miltiorrhiza was the best. Conclusion:The change of trace elements in Yu S. miltiorrhiza in different periods has certain regularity. Trace elements in soil have impacts on trace elements in medicinal materials. Trace elements in medicinal materials are closely correlated with index components and quality of medicinal materials.
RESUMO
Objective To determine the contents of elements Fe, Mn, Ni, Cu, Zn, Ca, Mg and Cr in Gentiana macrophlla and Gentiana officinalis. Methods The contents of elements were determined by flame atomic absorption spectrometry. Results The recovery rates obtained by standard addition method were between 88.1%～114.5%, and the RSDs were lower than 3.18%. Gentiana macrophlla is rich in the trace elements such as Fe, Ni, Cu, Ca, Mg, and Gentiana officinalis is rich in the trace elements such as Mn, Zn, Cr. Conclusion There are some difference between wild and planted Gentiana officinalis in element content, that may be related to their growing environment.
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Amounts of Certain elements in Anopheles sinensis, both normal ones (emerged mosquitoes, d0, d5, d8, d12 and d18 after taking blood meal) and those infected with Brugla malayi microfilaria (d5, d 8, d12 and d18 after infection), were determined using IL-551 and PE-603 atomic absorption spectrometer(AAS). The results showed that 9 kinds of trace elements including Fe, Zn, Cu, Cd, Al, Pb, Mn. Ni and Cr and 4 kinds of macro elements as K, Na, Ca, Mg were present in both the noninfected and infected mosquitoes. Comparing the contents of the elements between the noninfected and infected mosquitoes, the amounts of many kinds of elements reduced obviously in mosquitoes infected with microfilaria. The quantity and kinds of elements reduced in mosquitoes with the days of infection, for instance, 10 kinds of elements on the 5th day, 11 on the 8th day, 9 on the 12th day and 7 on the 18th day after infection(Tables 1, 2).