RESUMO
Aim To observe whether the mechanosensitive ion channel Piezo1 was involved in the senescence of atrial fibroblasts by activating β-catenin based on our previous study which found marked increase of Piezo1 mRNA in senescent atrial fibroblasts. Methods Primary mouse atrial fibroblasts (MAFs) were isolated from male C57BL/6 mice (3-4 weeks) by enzyme digestion, and tert-butyl hydroperoxide (TBHP) was used to induce the senescence of cells. The ratio of senescent cells was detected by senescence-associated β-galactosidase (SA-β-Gal) staining. The protein levels of Piezo1, β-catenin/p-β-catenin, senescence-associated proteins p53 and p21 in the cells treated with TBHP (100 μmol · L
RESUMO
OBJECTIVE: To observe the effects and mechanisms of sodium tanshinone II A sulfonate (STS) on transforming growth factor-β1 (TGF-β1)-induced atrial fibroblasts differentiation. METHODS: In the culture of neonatal rat atrial fibroblasts, type I/III collagen in the cell culture supernatant were measured by enzyme-linked immunosorbent assay. The proliferation activity was measured by methylthiazolyl tetrazolium(MTT) assay. Atrial fibroblasts differentiation was determined by measuring the expression of α-SMA(α-smooth muscle actin) using Western blot and immunofluorescence. Extracellular signal-regulated kinase 1/2 (ERK1/2) activation was measured by Western blot. RESULTS: STS can decrease TGF-β1-induced elevations of type I/III collagen and proliferation activity; inhibit the expression of α-SMA and ERK1/2 activation. CONCLUSION: SIS can inhibit TGF-β1-induced atrial fibroblasts differentiation, the mechanism may be associated with depression of ERKl/2 signaling pathway.