lnvestigation of correlations between vascular endothelial growth factor and proliferative diabetic retinopathy / 国际眼科杂志(Guoji Yanke Zazhi)

AlM:To determine the relations between the changes of vascular endothelial growth factor ( VEGF ) concentration in plasma, aqueous humor and vitreous of patients with diabetic retinopathy ( DR ) and proliferative diabetic retinopathy ( PDR) , as well as to find if the condition of metabolism control of patients with DR may affect the VEGF level. METHODS: Double antibody sandwich enzyme-linked immunosorbent assay was used to measure the levels of VEGF concentration in plasma of non-diabetic retinopathy ( NDR ) patients group, background diabetic retinopathy ( BDR ) patients group, and proliferative diabetic retinopathy ( PDR) patients group. The levels of HbAlc in plasma of patients in above three groups were measured at the same time. The VEGF concentration in aqueous humor, vitreous of PDR patients group and normal group was also measured for further comprehensive analysis. Human VEGF immunoassay Quantikine with high sensibility and quality was imported from R&D Co. LTD. , USA. RESULTS: There was an increasing tendency in the VEGF concentration in aqueous humor of PDR patients group. There was no statistically significant difference as it was compared with normal group (P>0. 05). The VEGF concentration in vitreous of PDR patients group was obviously increased, there was significant difference as it was compared with normal group (P 0. 05 ). ln normal group the VEGF concentration in plasma was the highest, but in PDR patients group the VEGF concentration in vitreous was the highest. CONCLUSlON: The significantly increased VEGF concentration in eyes, especially in vitreous of PDR patients may play an important role in development and deterioration of DR. lt was clear that VEGF played its normal biological functions mainly in plasma in normal human. ln severe DR patients, abnormal high may come from ischemic and VEGF concentration in vitreous suggested that it hypoxic retina and there was a tendency to diffuse towards the front of eyes.

Comparative Study on Measure Methods for Macular Thickness in Patients with Diabetic Retinopathy / 中国康复理论与实践

@#Objective To assess the value of Heidelberg retina tomograph-Ⅱ(HRT-Ⅱ)and optical coherence tomography(OCT)on making a diagnosis of macular edema in patients with background diabetic retinopathy.Methods Eighty eyes of 40 patients with background diabetic retinopathy were divided into the macular edema group and non-macular edema group according to examination of fluorescein fundus angiography(FFA).Macular thickness was measured by OCT and edema index was invested by HRT-Ⅱ.The results were analyzed by statistics test.Results The Kappa value of OCT examination on diagnosis for macular edema of background diabetic retinopathy was bigger than the HRT-Ⅱ.Making FFA as the diagnosis standard of macular edema,OCT examination showed P=0.0001,HRT-Ⅱ examination showed P=0.0856.Conclusion OCT and HRT-Ⅱ can be used to quantitate the macular edema of the diabetic retinopathy patients.OCT is better in the final diagnosis for macular edema than HRT-Ⅱ,and HRT-Ⅱ has higher misdiagnosis rate and lower missed diagnosis rate.HRT-Ⅱ can be used to screen macular edema patients with diabetic retinopathy.

Hue Discrimination and Contrast Sensitivity Deficits in Diabetic Subjects With and Without Retinopathy

In an attempt to assess the evidence of abnormal central vision before the development of either overt retinopathy or a reduction in visual acuity in patients with diabetes mellitus, hue discrimination and ccntrast sensitivity were tested in diabetic patients with background diabetic retinopathy(N=57) or no-retinopathy(N=28). The results were as follows: 1. There were statistically significant differences in the mean square root of total error score, blue-yellow and red-green partial error score between the normal controIs, the no-retinopathy group and the background diabetic retinopathy groups, respectively(P<0.001). 2. In the no-retinopathy group contrast sensitivity was decreased in high spatial frequency only in comparison with the normal controls. On the other hand, in the background diabetic retinopathy group contrast sensitivity was decreased in all spatial frequency in comparison with the normal controls and the no-retinopathy group(P<0.05). 3. The proportion of diabetic patients with statistically significant color vision and contrast sensitivity deficits in the no-retinopathy and the background diabetic retinopathy groups were 3.6% and 38.6% respectively.