Improvement effect and mechanism of Baicao fuyanqing suppository on bacterial vaginitis in rats / 中国药房

OBJECTIVE To explore the improvement effect and potential mechanism of Baicao fuyanqing suppository on bacterial vaginitis （BV） in rats. METHODS The female SD rats were randomly divided into normal group， model group， metronidazole group （positive control， 0.03 g/kg）， Baicao fuyanqing suppository low-dose， medium-dose and high-dose groups （0.18， 0.36， 0.72 g/kg）， with 8 rats in each group. Except for the normal group， the rats in other groups were injected subcutaneously with 0.2 g of Estradiol benzoate injection+20 μL of Escherichia coli suspension （2×108～3×108 cfu/mL） through the vaginal opening to establish the BV rat model. Administration groups were given relevant medicine vaginally， while the normal group and the model group were given normal saline， once a day， for 6 consecutive days. Twenty-four hours after the last administration， the vaginal appearance score and vaginal pH were measured for each group of rats. The levels of cytokines [interleukin-1β （IL-1β）， IL-2， IL-13， immunoglobulin A （IgA）] in vaginal lavage fluid were determined. The morphology of the uterus and accessories， and pathological changes in the vaginal tissue were observed. The expressions of Toll-like receptor 2 （TLR2）， TLR4 and nuclear factor-κB （NF-κB） in vaginal tissues were determined. RESULTS Compared with the normal group， the uterus edema， the irregular shape of uterus and accessories， the vaginal mucosa hyperplasia， and the massive desquamation of epithelial cells were observed in the model group， complicating with massive infiltration of inflammatory cells； vaginal opening redness and swelling score and secretion score， vaginal pH， the levels of proinflammatory cytokine IL-1β and IL-2， the protein expressions of TLR2， TLR4 and NF- κB were all increased or up-regulated， while the levels of IgA and anti-inflammatory cytokine IL-13 decreased significantly （P＜0.05 or P＜0.01）. Compared with the model group， varying cn degrees of improvement in uterine and accessories， and vaginal tissue lesions in rats were observed in administration groups， and most of the quantitative indicators mentioned above showed significant improvement （P＜0.05 or P＜0.01）. CONCLUSIONS Baicao fuyanqing suppository has a certain improvement effect on inflammatory symptoms in BV rats， and its mechanism may be related to the inhibition of the TLR/NF-κB signaling pathway.

Pharmacodynamics Studies of Yinyanjing Gel in the Treatement of Bacterial Vaginitis in Rats / 中国药师

To study the bacteriostat, anti-inflammatory and relieving itching effects of Yinyanjing gel. Methods: A double dilution method was used to detect the in vitro bacteriostat effect of Yinyanjing gel. The therapeutic effects of Yinyanjing gel on bacterial vaginitis were observed in rats. The antipruritic effect of Yinyanjing gel was observed in guinea pigs. Results:MIC for Staphy-lococcus aureus was 47. 39 mg·ml-1 , and that for Escherichia coli was 189. 59 mg·ml-1 . The therapeutic effects of Yinyanjing gel on bacterial vaginitis were significant. Yinyanjing gel showed notable antipruritic effect on the itching induced by histamine phosphate in guinea pigs. Conclusion:Yinyanjing gel exhibits significant effects of bactriostasis,anti-inflammation and relieving itching.

Curative efficacy analysis for metronidazole tablets combined with sophora gel in treatment of bacterial vaginitis / 中国生化药物杂志

Objective To observe the clinical efficacy of metronidazole tablets combined with sophora gel in treatment of bacterial vaginitis ( BV) . Methods Eighty-seven cases of BV patients were randomly divided into the study group(45cases) and the control group(42cases).The control group was given metronidazole tablets (400 mg, two times a day), oral for seven days, while the study group was given sophora gel (vaginal implantation) on the basis of control group.Seven days as a course of treatment.Pared the leukocyte esterase test positive rate in vaginal fluid and the recurrence rate within a year of the two groups before and after treatment.ResuIts After 7 days’treatment, the positive rate of leukocyte esterase (LE) in two groups were all significantly declined, which was more significant in the study group (P<0.05);the total effective rate of the study group was 95.6%, which was significantly higher than that of the control group 78.6%(P<0.05).The recurrence rate within a year of the study group was 6.7%, which was significantly lower than that of the control group 26.2%( P<0.05 ) .ConcIusion The therapy of metronidazole tablets combined with sophora gel in treating BV can significantly decline the LE positive rate and recurrence rate, and improve the clinical curative efficacy as well.

The pharmacoeconomic evaluations of four drugs in the treatment of bacterial vaginitis / 中国基层医药

Objective To investigate the cost-effectiveness of four drugs in treatment of bacterial vaginitis and improve clinic rational drug use.Methods 361 bacterial vaginitis out-patients were treated with lactobacillus vaginal effervescent tablets(92 cases),lacidophilin vaginal capsules(88 cases),shuangzuotai vaginal effervescent tablets(90 cases),metronidazole vaginal effervescent tablets(91 cases).The therapeutic effect,drug cost and therapeutic effect were evaluated by cost effectiveness analysis 7d after treatment.Results The overall response rate of lactobacillus vaginal effervescent tablets,lacidophilin vaginal capsules,shuangzuotai vaginal effervescent tablets,metronidazole vaginal effervescent tablets were 78.26％,76.14％,83.33％ and 78.02％,respectively.The overall response rate was no significant difference between four groups (0.06),and metronidazole vaginal effervescent tablets had the lowest cost effectiveness rate (C/E,0.26).Conclusion The metronidazole vaginal effervescent tablets is better schedule in treatment of bacterial vaginitis.

Clinical Implications of Multiplex PCR Detection of Fastidious Microorganisms in Vaginitis Patients / 대한임상미생물학회지

BACKGROUND: Bacterial vaginitis (BV) and Trichomonas vaginitis are the most frequently recurring infectious diseases in women. Therefore, accurate tests for post-treatment follow-up are required. A multiplex PCR assay allows for the simultaneous detection of multiple pathogens in a single specimen. In this study, we assessed the clinical implications of multiplex PCR detection of fastidious microorganisms causing vaginitis. METHODS: A total of 216 vaginitis patients who presented to Chung-Ang University Yongsan Hospital with more than one positive result on multiplex PCR (Trichomonas vaginalis (TV), Neisseria gonorrhoeae (NG), Chlamydia trachomatis (CT), Ureaplasma urealyticum (UU), Mycoplasma genitalium (MG), Mycoplasma hominis (MH)) were retrospectively enrolled in this study. Each patient's clinical symptoms, initial treatment and follow-up for BV, and other related test results were also retrospectively reviewed. RESULTS: The most commonly reported symptom was abnormal discharge, followed by pruritis (73.1%), lower abdominal pain (38.4%), urination difficulties (13%), and others such as fever. According to the multiplex PCR results, there were 116 cases (35.8%) of MH, 86 cases (26.5%) of UU, 62 cases (19.1%) of CT, and 84 cases (38.9%) were mixed infections. Among those patients with single infections, treatment changed for 63 cases (65.6%) while treatment remained unchanged for 17 (17.7%) after PCR results were reported. CONCLUSION: The diagnosis of BV using multiplex PCR is clinically effective and the results of which can be incorporated in antibiotic selection for patients with multiple sexually transmitted diseases (STD). Multiplex PCR may be especially helpful in the diagnosis of patients in whom the differentiation of STD pathogens is difficult using traditional methods.

Therapeutical Effect of Zangfukang Suppository on Experimental Vaginitis in Rats / 中国药房

OBJECTIVE: To evaluate the therapeutic effect of Zangfukong suppository on the experimental vaginitis.METHODS:The experimental rats were equally assigned to 6 groups: normal control group,model control group,gyno-daktarin(0.12 g?kg-1) group,three Zangfukang groups(0.33 g,0.17,and 0.08 crude drug? kg-1).Candida albicans solution was injected into the vigina of the estrous rats to establish colpomycosis model.The effect of Zangfukang suppository on the infection rate of candida albicans was observed.The bacterial vaginitis model was established by injecting the mixture bacteria of Neisseria gonorrhoeae,staphylococcus aureus and escherichia coli into the vagina of rats,and the effect of Zangfukang suppository on the gonococcal infection rate.RESULTS: After local application of Zangfukang suppository for 7 consecutive days for treatment of colpitis mycotica of rats,the infection rates of candida albicans were significantly lower in medium-dose and high-dose groups than in the model control group(P

Bacterial vaginitis infection in pregnant women

The prevalence of Bacterial vaginitis in pregnant women was 7.8%. 42.6% women with Bacterial vaginitis are asymptomatic. Risks associated with Bacterial vaginitis include history of IUD use (OR: 3.41; 95% Cl: 1.65-6.99) and changing pads under three times a day (OR: 2.19; 95% Cl:1.07-4.45). Amoxicillin is effective for treatment of Bacterial vaginitis in pregnant women.