Study of bacteriological pattern of suspected cases of meningitis.

Meningitis is an inflammatory condition of the membranes that cover the brain and spinal cord. Present study was carried out to know The pattern of bacteriology of suspected cases of meningitis and to find the rate of susceptibility of various age groups. This work is a bacteriological, cytological and biochemical study of cerebrospinal fluid collected from patients clinically suspected of meningitis, and admitted in Civil hospital, Ahmedabad during the period of 5th April 1999 to 11th May 2000. Higher incidences (34 %) of bacteriological positive cases were found among the patients below one year of age. Most commonly isolated organisms was S.aureus (54.1 %) followed by E.coli and klebsiella. Study shows mortality rate of 29.16 %

A surveillance study of bacteriologic profile in rhinosinusitis.

Objective: To review the types of bacteria found in rhinosinusitis and the prevalence of a beta-lactamase producing organism in a tertiary care hospital during the year 2004 and compared with our previous reports. Methods: Charts of patients who underwent endoscopic sinus surgery or maxillary antral puncture or endoscopic-guided culture at the Rhinology & Allergy Division, Department of Otolaryngology and the Department of Microbiology, Siriraj Hospital from January 2004 to December 2004 were reviewed. Information regarding the patient’s age, site of specimens, culture and sensitivity results were obtained. Results: There were 162 specimens and 29 bacterial species isolated. There were 50.4% positive aerobic cultures, gram-negative bacteria were more common than gram-positive bacteria (68.5% vs 31.5%). Common aerobes were Pseudomonas aeruginosa (16.2%), non-fermentative gram negative rod: NF-GNR (10.8%), Coagulase- negative Staphylococcus aureus :CNS (9.9%) and Klebsiella pneumoniae (9.9%).The most common anaerobes were Peptostreptococcus sp., Bacteroides fragilis and Fusobacterium sp. Conclusion: Contrary to our previous studies, gram negative organisms play a more important role than gram positive organisms. The causative pathogens of rhinosinusitis should be studied continuously because rapid progress in the development of new antimicrobial agents has a significant impact on their bacteriologic profile.

Bacteriological and serological study of clinical cholera: a report on 94 cases admitted at the San Lazaro Hospital

Out of 94 cases with a clinical diagnosis of cholera, only 69.15% could be bacteriologically proven. The pitfalls in the clinical diagnosis is in the less severe casesNCV, which had been attributed by many authors as possible cause of diarrheal disease in man does not seemed to be true with the three isolates in this studyThere was a fairly close correlation between the bacteriological and the serological findings in this study, which confirmed results of other workers. However, while the bacteriological results could be affected by antimicrobial, the serological was not. A combined bacteriological and serological study of cases with clinical manifestations of cholera would therefore results in a greater percentage of confirmed cases. (Summary)

Cross Contamination of Inpatient Bed Sheet: An Evaluation / 中华医院感染学杂志

OBJECTIVE Through studying the bacteria spreading in the in-hospital patients′ mattresses,cotton quilts,and pillows,to analyze the possible factors that could lead to the cross-infections among the(patients).METHODS Using the technique stipulated in the Regulations of Sterilization Techniques(Ministry of Health,2002) GB15982-1995A_3,30 samples were collected from each group of mattresses,cotton quilts,and pillows,and were examined through bacteriological lab investigations.RESULTS There were different types of(bacteria) in the mattresses,cotton quilts,and pillows;the spread of the bacteria was various.CONCLUSIONS The(percentage) of infection in the pillows is higher than the percentage in the mattresses and the cotton quilts.There are different types of bacteria existing in the samples taken,and the spreading of the bacteria varies.

Epidemilogical Study of Nares and Umbilicus of Normal Fullterm Newborns in the Nursery and Clinical Follow Up at 4 Weeks of Age

This study was undertaken to find out the bacteriological colonization of nares and umbilicus of 166 normal fullterm newborns in the nursery of Ewha Womans University Hospital during the period from July 1 to Aug 31. 1980 and its relation with follow up study at the age of 4 weeks. The results are as following: 1) The cultures of nares and umbilicus of 91 newborns, while only soap was used(Group 1), were compared with those during the period when Betadine was added as a washing agent(75 newborns, Group 2). 2) The incidence of colonization with coagulase (+) staphylococci was 28.6% in nares, and 29.7% in umbilicus(average 29.1%) in Group 1, and 20.0%, 13.3% respectively(average 16.0%) in Group 2. 3) In follow up, 33.3% of Group 1 and 42.7% of Group 2 were seen at 4 weeks of age. Among them, the incidence of pyoderma was 3.3% in (Group 1) and 6.1% in Group 2, and that of omphalitis was 3.3% and 6.1% in each Group respectively. 4) The incidence of clinical infection at 4 weeks of age was 1.5% in culture-negative 117 cases in Group 1, and 2.2% in culture-positive 45 cases, 2.9% in culture-negative 105 cases in Group 2. 5) Gram-Negative bacilli was cultured in 7.1% of Group 1 and 14.7% of Group 2. Lactose-fermenter, e.g. E. coli, comprised 61.5% in Group 1 and 50.0% in Group 2, and non-lactose fermenter, e.g. Pseudomonas, comprised the rest. 6) Gram negative bacilli was recovered in 1.9% of Staphylococci-positive population and 8.5% of Staphylococci-negative population in Group 1. In Group 2, the incidence of gram-negative bacilli culture was 15.4% in Staphylococci-positive and 14.5% in Staphylococci negative population respectively.

Bacteriological Study of Urinary Tract Infections / 대한비뇨기과학회지

Urine specimens were obtained from one hundred and thirty nine patients in-out-patient and in-patient service of B.N.U.H., Dept. of Urology, with the presumptive diagnosis of urinary tract infections or indwelling catheter. These specimens were processed by Triphenyltetrazolium Chloride test, quantitative test and identification of the organisms. The results were as follows: 1. Triphenyltetrazolium Chloride test was an easily performable and a fairmely accurate method for screening of urinary tract infections. 2. The urine specimens showed false negative reactions to triphenyltetrazoium Chloride in some cases with moderate (10(4) to 10(6) bacteria per milliliter) growth of bacteria or in the antibiotics used patients. 3. The incidence of urinary tract infections with gram negative bacilli are ninety-two percent. In their series, incidence of E. coli decreased and that of other gram negative bacilli increased such as Enterobacter, Proteus and Serratia, etc. This shift in the pathogen spectrum occurs mostly in the patients of complicated or lower urinary tract infections, in-patients, antibiotics used patients and the patients with indwelling catheter.