Effect of Dietary Supplementation of Critical Amino Acids and Multi-Enzyme with Low Protein and Energy Diet on Carcass and Blood Biochemical Performance of Broiler Chicken

The experiment was conducted to study the effect of dietary supplementation of critical amino acids and multi-enzyme with reduced levels of energy and protein diet on carcass and blood biochemical performance in broiler chicken. Three hundred broiler chicks were allotted to five dietary treatments of three replications and 20 birds in each replication. The control (T0) were fed with standard diet, T1 (5% low CP and energy than standard with balancing of lysine and methionine), T2 (T1 + Multi-enzyme), T3 (10% low CP and energy than standard with balancing of lysine and methionine), T4 (T3 + Multi-enzyme).The dressing percentage and edible meat percentage were significantly more in T2 group. The breast meat percentage of the control group was comparable with T2 group. The result showed that the significantly higher (P<0.05) gizzard weights in groups receiving enzyme combination supplementation as compared to control group (T0) irrespective of the energy-protein levels. The mean value of total protein, albumin, globulin and blood urea nitrogen of all the experimental birds were found to be statistically similar with that of the control group. The treatment group T2 found to be effective and economical

Ameliorative potentials of aqueous extracts of leaf and stem of Ipomoea involucrata on selected biochemicals in experimental diabetic rats

Background: Diabetes mellitus is a chronic metabolic disorder characterized by derangements in carbohydrate, protein and lipid metabolisms, due to deficiency in insulin secretion and action. This research evaluates the ameliorative potentials of aqueous extracts of leaves and stem of Ipomoea involucorata on selected biochemicals in experimental diabetic rats.Methods: Diabetes mellitus was induced by single intraperitoneal injection of 150 mg/kg body weight of alloxan and the animals were orally administered with gilanil (4 mg/kg) for positive control, 100, 200 and 300 mg/kg bw aqueous extract of leaves (groups 4-6) and stem (groups 7-9) of Ipomoea involucrata once daily for 21 days. Biochemical parameters were analysed using standard methods.Results: The median lethal dose was established at 648 mg/kg (leaves) and 547 mg/kg (stem). The negative group (untreated) showed significant increase in glucose concentration compared to the other groups. After 2 to 3 weeks there was significant (p<0.05) decrease in glucose concentration of the extract and glibenclamide (positive group) treated groups when compared with the negative group. Diabetes control rats showed significant (p<0.05) high serum lipid profile (except for high density lipoprotein), liver enzymes/ indices and renal indices when compared with non-diabetic control rats. However, these alternations were reversed with the positive group and the groups treated with aqueous extracts of both samples. The differences observed in the electrolytes were not significant in all groups.Conclusions: The results suggest that aqueous extract of leaves and stem of I. involucrata is considerably safe and a potential therapy for management of complications associated with diabetes mellitus.

Analgesic and side effects of intravenous recombinant Phα1ß

Intrathecal injection of voltage-sensitive calcium channel blocker peptide toxins exerts analgesic effect in several animal models of pain. Upon intrathecal administration, recombinant Phα1ß exerts the same analgesic effects as the those of the native toxin. However, from a clinical perspective, the intrathecal administration limits the use of anesthetic drugs in patients. Therefore, this study aimed to investigate the possible antinociceptive effect of intravenous recombinant Phα1ß in rat models of neuropathic pain, as well as its side effects on motor, cardiac (heart rate and blood pressure), and biochemical parameters. Methods: Male Wistar rats and male Balb-C mice were used in this study. Giotto Biotech® synthesized the recombinant version of Phα1ß using Escherichia coli expression. In rats, neuropathic pain was induced by chronic constriction of the sciatic nerve and paclitaxel-induced acute and chronic pain. Mechanical sensitivity was evaluated using von Frey filaments. A radiotelemeter transmitter (TA11PA-C10; Data Sciences, St. Paul, MN, USA) was placed on the left carotid of mice for investigation of cardiovascular side effects. Locomotor activity data were evaluated using the open-field paradigm, and serum CKMB, TGO, TGP, LDH, lactate, creatinine, and urea levels were examined. Results: Intravenous administration of recombinant Phα1ß toxin induced analgesia for up to 4 h, with ED50 of 0.02 (0.01-0.03) mg/kg, and reached the maximal effect (Emax = 100% antinociception) at a dose of 0.2 mg/kg. No significant changes were observed in any of the evaluated motor, cardiac or biochemical parameters. Conclusion: Our data suggest that intravenous administration of recombinant Phα1ß may be feasible for drug-induced analgesia, without causing any severe side effects.(AU)

Analgesic and side effects of intravenous recombinant Ph alfa 1 beta

Background: Intrathecal injection of voltage-sensitive calcium channel blocker peptide toxins exerts analgesic effect in several animal models of pain. Upon intrathecal administration, recombinant Phα1β exerts the same analgesic effects as the those of the native toxin. However, from a clinical perspective, the intrathecal administration limits the use of anesthetic drugs in patients. Therefore, this study aimed to investigate the possible antinociceptive effect of intravenous recombinant Phα1β in rat models of neuropathic pain, as well as its side effects on motor, cardiac (heart rate and blood pressure), and biochemical parameters. Methods: Male Wistar rats and male Balb-C mice were used in this study. Giotto Biotech® synthesized the recombinant version of Phα1β using Escherichia coli expression. In rats, neuropathic pain was induced by chronic constriction of the sciatic nerve and paclitaxel-induced acute and chronic pain. Mechanical sensitivity was evaluated using von Frey filaments. A radiotelemeter transmitter (TA11PA-C10; Data Sciences, St. Paul, MN, USA) was placed on the left carotid of mice for investigation of cardiovascular side effects. Locomotor activity data were evaluated using the open-field paradigm, and serum CKMB, TGO, TGP, LDH, lactate, creatinine, and urea levels were examined. Results: Intravenous administration of recombinant Phα1β toxin induced analgesia for up to 4 h, with ED50 of 0.02 (0.01-0.03) mg/kg, and reached the maximal effect (Emax = 100% antinociception) at a dose of 0.2 mg/kg. No significant changes were observed in any of the evaluated motor, cardiac or biochemical parameters. Conclusion: Our data suggest that intravenous administration of recombinant Phα1β may be feasible for drug-induced analgesia, without causing any severe side effects.

Analyzing the bioassay of biochemical drugs on the Chinese Pharmacopoeia 2015 / 中国生化药物杂志

To analyze the major changes and existing problems of bioassay methodrelated to specifications of biochemicalsin the Chinese Pharmacopoeia 2015 edition and to discuss the trend of the quality control of biochemical and bioassay method improved

Influence of pulsed magnetic field on soybean (Glycine max L.) seed germination, seedling growth and soil microbial population.

The effects of pulsed magnetic field (PMF) treatment of soybean (Glycine max L. cv CO3) seeds were investigated on rate of seed germination, seedling growth, physico-chemical properties of seed leachates and soil microbial population under laboratory conditions. Seeds were exposed to PMF of 1500 nT at 0.1, 1.0 10.0 and 100.0 Hz for 5 h per day for 20 days, induced by enclosure coil systems. Non-treated seeds were considered as controls. All PMF treatments significantly increased the rate of seed germination, while 10 and 100 Hz PMFs showed the most effective response. The 1.0 and 10 Hz PMFs remarkably improved the fresh weight of shoots and roots, leaf area and plant height from seedlings from magnetically-exposed seeds compared to the control, while 10 Hz PMF increased the total soluble sugar, total protein and phenol contents. The leaf chlorophyll a, b and total chlorophyll were higher in PMF (10 and 100 Hz) pretreated plants, as compared to other treatments. In addition, activities of α-amylase, acid phosphatase, alkaline phosphatase, nitrate reductase, peroxidase and polyphenoloxidase were increased, while β-amylase and protease activities were declined in PMF (10 Hz)-exposed soybean plants. Similarly, the capacity of absorbance of water by seeds and electrical conductivity of seed leachates were significantly enhanced by 10 Hz PMF exposure, whereas PMF (10 Hz) pretreated plants did not affect the microbial population in rhizosphere soil. The results suggested the potential of 10 Hz PMF treatment to enhance the germination and seedling growth of soybean.