RESUMO
【Objective】 To explore the quantitative value of key points for ABO blood group initial screening in fixed blood donation sites, so as to provide reference for standardized testing process of sliding method. 【Methods】 Several groups of experiments were carried out to illustrate the optimal conditions, including the serum dosage of monoclonal reagent, red blood cell dosage of blood sample, and reaction time in ABO blood group initial screening, by sliding method, and the quantitative value of key points of sliding method was preliminarily determined. Blood typing tests of 310 blood donor samples including type A, B, O, AB, subtype A and subtype AB were conducted to evaluate the effects of quantitative value of key points in the initial screening procedure. The test tube method would be conducted if the results are inconsistent with the fully automated blood grouping analyzer. The ABO subtypes suspected are identified by serological and molecular biological methods. 【Results】 The quantitative value of key points in initial screening procedure of sliding methods was as follows: 2 drops of reagent serum, 5-10 μL of whole blood and 3 minutes of reaction time. The concordance rate of ABO blood type screening comparison experiment in 310 blood donors was 100%. 【Conclusion】 ABO blood group initial screening by sliding method with quantitative value can effectively standardize the pre-donation blood type screening in fixed blood donation sites, and can meet the requirements of ABO blood group initial screening.
RESUMO
【Objective】 To apply the spatial distribution analysis based on ArcGIS software, which has been applied widely in other fields, so as to analyze the intended locations for whole blood donation. 【Methods】 After a random stratified sampling was conducted among blood donors in the 17 donation sites during August 1st, 2021- July 30th, 2022, their intended blood donation locations were collected by an e-questionnaire. Addresses of donors′ intended donation locations were derived for GCJ-02 coordinates form and transformed by pandas module of Python to WGS84 coordinates, which further loaded to ArcGIS Arcmap module using Grouping Analysis for 17 median centers. The addresses of 17 blood donation sites in Guangzhou Blood Center were transformed to WGS84 coordinates and loaded to ArcGIS Arcmap module using the same methods for 3 ring buffer analysis. The criterion for judging whether the two were " matched" was whether the intended blood donation sites were covered by or adjacent to the 3 ring buffer zone of the existing blood donation sites. 【Results】 Of the 17 potential sites obtained from the spatial distribution analysis of 40 523 valid addresses of donors, 8 sites were covered or adjacent to the buffer of the existing donation sites, while the other 9 sites were far away from the existing donation sites. 【Conclusion】 By analyzing the spatial distribution of donors′ intended donation addresses, we can find out the service needs of donors for donating blood conveniently, which can provide basis for further blood donation service optimization.
RESUMO
【Objective】 To explore the whole blood collectionstatusat street fixed blood donation sites in different time periods and regions, among different populations with different recruitment methods in Chengdu. 【Methods】 The whole blood donation data at street fixed blood donation sites in Chengdu from 2014 to 2018 were collected, and then statistically analyzed in terms of weekdays/ weekends/holidays, the urban/suburban counties, the first/ repeated blood donation and local/ non-local household registration.On weekdays, blood donors were recruited at fixed blood donation sites while on weekends/holidays they were recruited via on-site recruitment combined with "computer-network-mobile phone" mode. 【Results】 There are 36 fixed blood donation sites in Chengdu, among which 22 were in suburban counties and 14 in urban area.There were more blood drives on weekdays than weekends / holidays every year, with fewer average daily collection on weekdays in comparison with weekends/holidays(P<0.05). The collection units on weekends/holidays were high in January, July, August and December, medium in March, April, June, September and October, and low in February, May and November (P<0.05). There were more blood drives in urban area than suburban counties on weekends/holidays, with fewer average daily collection in urban areain comparison with suburban counties. From 2014 to 2018, the total collection units at street fixed blood donation sites in suburban counties increased year by year (P<0.05). The proportion of first-time blood donors in urban area (59.62%) was significantly higher than that in suburban counties (43.45%) (P<0.05). The proportion of non-local registered blood donors in urban area (53.39%) was significantly higher than that in suburban counties (20.39%)(P<0.05). The number of text messages we sent for recruitment on weekends and holidays and the number of blood donors who responded increased year by year (P<0.05). 【Conclusion】 Whole blood collection at street fixed blood donation sites in Chengdu suburban counties on weekends and holidays was enhanced by using information interactive mode as "computer-network-mobile phone", which allows blood station managers to schedule blood collection in different periods and formulate reasonable blood collection plan.