Simultaneous Determination of Ferulic Acid and Tetramethylpyrazine in Rabbit Serum by RP-HPLC / 中国药房

OBJECTIVE:To establish a method for simultaneous determination of ferulic acid(FA)and TMP in serum sample.METHODS:After deprotein with boiling water-bath,RP-HPLC was performed on ODS C 18 column(250mm?4.6mm)with methanol-water(containing0.1%acetic acid)=8∶17as the mobile phase and UV detection at280nm.RESU LTS:The calibration curve of FA was linear within the range of30.2～70.8?g/ml,Y=57.51+413.12X,r=0.9974;the curve of TMP was linear within the range of10.1～50.7?g/ml,Y=41.32+308.64X,r=0.9922.CONCLUSION:Compared with the traditional extraction,the method is simple,rapid and sensitive,and can be used for simultaneous determination of the contents of active compositions in Chinese materia medica.