An Experimental Evaluation of humboldtia vahliana wight., a Folk Medicinal Plant on Wound Healing Action in Wistar Albino Rats-an in-vivo Study

A wound is a break in the integrity of the skin or tissues often which may be associated with disruption of the structure and function. Globally, the magnitude of acute wounds as a healthcare problem is sharply rising mainly due to industrialization. There is still a need to revisit the ancient healing methods by using traditional medicine aiming to discover a wound healing drug with better protection, rapid granulation changes, least side effects, good bio-availability and low cost. A folk medicinal plant Humboldtia vahliana Wight. belongs to the family Fabaceae, is a medium sized tree with rough, dark brown bark mottled with white. Its stem bark is used for wound healing among the tribes. Aims and objectives: The study aims to evaluate the in-vivo wound healing action of Humboldtia vahliana Wight. through excision and incision animal wound models. Methodology: 36 male Wistar albino rats weighing 150-250gm. were selected and made into 3 groups each for both incision and excision wounds, i.e., control, standard and trial group -each containing 6 rats. The trial drug Humboldtia vahliana Wight. was applied over the wound area in the form of ‘Kalka’. In excision wound model, percentage of wound contraction was the parameter for the study and was achieved by using Planimetry. In incision wound model, tensile strength was the parameter and was achieved by Local Tensiometer. Results: In case of both Incision as well as Excision wound contraction study, the data shows a statistically non-significant increase in percentage wound contraction of Trial group when compared to Control and Standard groups. Interpretation and Conclusion: The stem bark of Humboldtia vahliana Wight. showed better wound healing action in Wistar albino rats when percentage of efficacy or mean values were compared

Effect of varying dietary crude protein and metabolizable energy levels on leg abnormalities and performance in broiler chickens

Background: Nutrient restriction is a common strategy to prevent metabolic disorders in fast growing broiler chickens, but limited information is available regarding the impact of diets with low protein and energy on leg abnormalities Objective: Two experiments were conducted to evaluate the effect of varying crude protein (CP) and metabolizable energy (ME) levels on gastrocnemius tendon (GTeBS) and tibia breaking strength (TiBS), gait score (GS) and valgus/varus angulation (VAng). Methods: In Experiment 1, eight-d-old Ross 308 broilers (n=90) were randomly assigned into three treatments: 13/2,900 (13% CP and 2,900 kcal ME kg-1 of diet), 17/3,000 (17% CP and 3,000 kcal ME kg-1 of diet), and 21/3,025 or control (21% CP and 3,025 kcal ME kg-1 of diet). In Experiment 2, six-d-old Ross 308 chickens (n=192) were randomly distributed into two treatments: 16/3,000; 16% CP and 3,000 kcal ME kg-1 of diet and 21/3,000 or control; 21% CP and 3,000 kcal ME kg-1 of diet. In both experiments data were analyzed as one-way ANOVA. Results: In Experiment 1, broilers in the 17/3,000 and control treatments had similar (p>0.05) GTeBS (202 and 224 N, respectively), and TiBS (338 and 332 N, respectively). Birds in the 13/2,900 treatment showed higher GS (lower walking ability; 80% of birds with score >3), and greater VAng (53% of birds with score >2) than broilers in the control treatment (0% of birds with GS score >3 and 0% of birds with VAng score >2). Experiment 2, broilers in the 16/3,000 showed similar VAng, GS and TiBS than birds in the control treatment. However, birds in the 16/3,000 treatment showed lower (-26%) GTeBS than birds in the control treatment (p<0.05). Conclusions: Feeding broilers with 16% CP and 3,000 kcal ME kg-1 of diet did not affect tibia breaking strength, gait score and valgus/varus angulation. A diet containing 16% CP or less and 2,900-3,000 kcal ME kg-1 reduces tendon and tibia breaking strength.

Antecedentes: La restricción de nutrientes es una estrategia común para prevenir trastornos metabólicos en pollos de engorde, sin embargo, se dispone de información limitada sobre el impacto de las dietas bajas en proteína y energía en las anomalías de piernas. Objetivo: Se llevaron a cabo dos experimentos para evaluar el efecto de niveles variables de proteína cruda (PC) y energía metabolizable (EM) en la fuerza de rotura del tendón del gastrocnemio (GTeBS) y tibia (TiBS), la evaluación de marcha (GS) y la angulación en valgus/varus (VAng). Métodos: En el Experimento 1, pollos de engorde Ross 308 de ocho días de edad (n = 90) se asignaron al azar en tres tratamientos: 13/2.900; 13% PC y 2.900 kcal ME kg-1 de dieta, 17/3.000; 17% PC y 3.000 kcal ME kg-1 de dieta y 21/3.025 o control; 21% PC y 3.025 kcal ME kg-1 de dieta. En el Experimento 2, pollos Ross 308 de seis días de edad (n = 192) se distribuyeron aleatoriamente en dos tratamientos: 16/3.000; 16% PC y 3.000 kcal ME kg-1 de dieta y 21/3.000 o control; 21% PC y 3.000 kcal ME kg-1 de dieta. En ambos experimentos los datos se analizaron como ANOVA de una vía. Resultados: En el Experimento 1, los pollos de los tratamientos 17/3.000 y control tuvieron GTeBS (202 y 224 N, respectivamente) y TiBS (338 y 332 N, respectivamente) similares (p>0,05). Las aves del tratamiento 13/2.900 mostraron mayor GS (menor capacidad para caminar; 80% de las aves con puntaje >3) y mayor VAng (53% de las aves con puntaje >2) que los pollos del tratamiento control (0% de aves con un puntaje GS >3 y 0% de aves con un puntaje VAng >2). En el Experimento 2, los pollos del 16/3.000 mostraron VAng, GS y TiBS similares a los de las aves del tratamiento control. Sin embargo, las aves del tratamiento 16/3.000 mostraron menor (-26%) GTeBS que las aves control (p<0,05). Conclusiones: La alimentación de pollos de engorde con 16% PC y 3.000 kcal EM kg-1 de dieta no afecta la resistencia a la ruptura de la tibia, la marcha y la angulación valgus/varus. La dieta formulada con 16% de PC o menos y 2.900-3.000 kcal de EM kg-1 reduce la resistencia a la ruptura del tendón y de la tibia.

Antecedentes: A restrição de nutrientes é uma estratégia comum para prevenir distúrbios metabólicos em frangos de corte, no entanto, há informações limitadas disponíveis sobre o impacto de dietas de baixa proteína e baixa energia nas anormalidades de pernas destas aves. Objetivo: Dois experimentos foram realizados para avaliar o efeito de diferentes níveis de proteína bruta (PB) e energia metabolizável (EM) em força de ruptura do tendão do músculo gastrocnêmio (GTeBS), forca de ruptura da tíbia (TiBS), gait score (GS) e angulação em valgus/varus (VAng). Métodos: Experimento 1, foram utilizados 308 frangos de corte machos da linhagem Ross com oito dias de idade (n = 90) distribuidos aleatoriamente em três tratamentos: dieta controle com 21% PB e 3.025 kcal EM kg-1 13/2.900; dieta con 13% PB e 2.900 kcal EM kg-1, y 17% PB e 3.000 kcal EM kg-1 . Experimento 2, foram utilizados 308 frangos de corte da linhagem Ross com seis dias de idade (n = 192) distribuídos aleatoriamente em dois tratamentos: dieta controle con 21% PB e 3.000 kcal EM kg-1 e dieta com 16% PB e 3.000 kcal EM kg-1 . Em ambos os experimentos, os dados foram submetidos a ANOVA. Resultados: Experimento 1, os frangos de corte submetidos ao dos tratamentos 17/3.000 e controle nao apresentaram diferencas significativas (p>0,05) para as variáveis GteBS (202 y 224 N, respectivamente) e TiBS (338 y 332 N, respectivamente). Os frangos submetidos ao 13/2.900 apresentaram maior frequência de GS (menor capacidade de locomocao; 80% das aves com pontuação>3) e maior VAng (53% das aves com pontuação>2) do que os frangos do tratamento controle (0% das aves com pontuação GS>3 e 0% das aves com pontuação VAng>2). Experimento 2, os frangos do 16/3.000 apresentaram VAng, GS e TiBS semelhantes aos das aves do tratamento controle. Entretanto, as aves do tratamento 16/3.000 apresentaram GTeBS mais baixo (-26%) em comparacao as aves do tratamento de controle (p<0,05). Conclusões: A dieta com 16% PB e 3.000 kcal EM kg-1 não afetou a forca de ruptura da tíbia, gaite score marcha e angulação em valgus/varus de frangos de corte. Uma dieta com níveis de 16% de PB ou menos e 2.900-3.000 kcal EM kg-1 reduziu a resistência à ruptura do tendão e a resistência de tibia de frangos de corte.

1, 25-dihidroxivitamina-D 3 sobre as características ósseas de frangos de corte fêmeas / 1, 25-dihydroxyvitamin-D3 over bony characteristics of female broilers

Objetivou-se avaliar as características dos tibiotarsos de frangos de corte fêmeas. As variáveis analisadas foram os pesos in natura, secos e desengordurados, o comprimento, os diâmetros, a resistência óssea, o índice de Seedor (IS), os percentuais de proteínas colagenosas (PC), as proteínas não colagenosas (PNC), os minerais (cálcio, fósforo, potássio e sódio) e as cinzas. Foram utilizadas 648 aves, da marca comercial Cobb®, em um delineamento em blocos ao acaso, com seis tratamentos e seis repetições, com 18 aves por unidade experimental. Os tratamentos consistiram na suplementação de 0,00; 0,50; 1,00; 1,50; 2,00 e 2,50 µg de 1,25-dihidroxivitamina-D3 (1,25(OH)2D3)/kg de ração. Aos 21 e 35 dias de idade, as aves foram pesadas e uma ave por unidade experimental (UE) com o peso médio da UE foi eutanasiada para a obtenção dos tibiotarsos e subsequente análise dos parâmetros ósseos. As variáveis métricas, bem como a composição orgânica (PC), a densidade (IS) e a resistência à quebra dos ossos das aves, não foram influenciadas pelos tratamentos. No recebimento de 2,50 µg de 1,25(OH)2D3/kg de ração, observou-se maior retenção mineral (cinzas) nos ossos das aves aos 35 dias de idade.(AU)

This study aimed to evaluate the characteristics of tibiotarsus of female broilers. The variables analyzed were the weights in natura, dry and degreased, length, diameter, bone strength, Seedor index, percentage of collagenous protein (CP), non-collagenous proteins (NCP), minerals (calcium, phosphorus, potassium and sodium), and ash. Six hundred forty-eight, Cobb® birds were used, a design of randomized blocks with six treatments and six replicates of 18 birds each. The treatments consisted of supplementation of 0.00; 0.50; 1.00; 1.50; 2.00 to 2.50 µg of 1,25-dihydroxyvitamin-D3 (1,25(OH)2D3)/kg of ration. At 21 and 35 days of age the birds were weighed and a unit/experimental unit (EU) with the EU average weight was euthanized to obtain the tibiotarsos and subsequent analysis of the bone parameters. The metric variables, as well as the organic composition (CP), density (IS) and resistance to breakage of the bones of the birds were not affected by treatments. Upon reception of 2.50 µg of 1,25(OH)2D3/kg ration, there was a higher mineral retention (ashes) in the bones of female broilers at 35 days of age.(AU)

Effects of shilajit on the bone tissue of alcohol administered rats.

Purpose: The purpose of the current study was to examine the effect of Shilajit; a herbomineral, supplementation on the mechanical strength of alcohol treated rat bone. Methods: Experimental animals each were assigned to six groups: group A(control): control rats were given water orally for a period of ten weeks; group B (treated): Animals were given processed shilajit (PS; 100mg/kg/day); group C (treated): Animals were given processed shilajit (PS; 200mg/kg/day); group D: animals were given 30% alcohol; group E: animals were given 30% alcohol and shilajit (100mg/kg/day) orally; group F: animals were given 30% alcohol and shilajit (200mg/kg/day) orally for ten weeks. Bone tissue mechanical strength along with bone weight, liver antioxidative enzymes and alkaline phosphatase (ALP) were assessed for all the treatment groups. Results: Mechanical strength of the bone tissue (both femur as well as tibia) was found to be significantly enhanced upon shilajit supplementation to alcohol treated group. Also, the activities of anti oxidant enzymes and alkaline phosphatase in the liver of alcohol administered groups were restored upon shilajit administration. Conclusion: These findings suggest that shilajit is very efficacious and competent in the maintenance of bone health

Evaluation of the wound healing effect of Jasminum grandiflorum by wound contraction studies and wound breaking strength.

Background: Wound is a common process leading to inflammation and healing process. Jasminum grandiflorum is a plant widely grown in Karnataka and is mention in ancient medical systems to promote healing. So the study was taken up to confirm the anti-inflammatory effect of the plant. Methods: Excision and incision wound models in albino rats were used to study the wound healing effect. It was mainly studied by the degree of epithelisation at various intervals and compared with control group. The strength of the wound was assessed by its wound breaking strength at the end. Results: It was found that jasmine extract increased the degree of epithelisation and thus promoting wound healing. It was also found that the wound breaking strength was more in the drug treated group compared to normal. Conclusions: Jasminum grandiflorum leaf extract is known to have wound healing property.

Fermented soybeans by Rhizopus oligosporus reduce femoral bone loss in ovariectomized rats

BACKGROUND/OBJECTIVES: Soy isoflavones are structurally similar to estrogen and bind to estrogen receptors, suggesting that they exhibit estrogenic activities; therefore, they are referred to as phytoestrogens. Fermentation may affect the bioavailability of isoflavones altering soy isoflavone glycosides in the form of aglycones. Thus, this study investigated the effects of fermented soybeans by Rhizopus oligosporus on bone metabolism in both young rats as a pilot test and in ovariectomized (ovx) old rats as a model of menopause. MATERIALS/METHODS: In the pilot test, a total of 24 seven-week-old female Sprague-Dawley (SD) rats were fed one of three diets for a period of four weeks: casein, unfermented soybean product, or fermented soybean product by R. oligosporus. In the ovx rat model, 20-week-old SD rats weighing 260-290 g underwent either sham-operation (n = 10) or bilateral ovariectomy (n = 30) and were then fed the AIN-93M diet for one week. Thereafter, rats were fed sham-casein, ovx-casein, ovx-soybean, or ovx-fermented soybean diet for five weeks. After decapitation, femoral bones were isolated and preserved in 9% formalin for assessment of bone mineral density (BMD), bone mineral content (BMC), and bone-breaking strength (BBS). RESULTS: Ovx rats showed significantly increased weight gain and decreased uterine wet weight. Of particular interest, ovx rats fed fermented soybeans showed increased uterine wet weights compared to control rats. Fermented soybean diet caused a significant increase in plasma 17-beta estradiol concentrations in young rats, and 17-beta estradiol levels were enhanced in ovx rats to match those of sham-operated ones. Significantly lower femoral BMD and BMC were observed in ovx rats compared to sham-operated controls, whereas bone areas did not differ statistically among the groups. In addition, BBS tended to be increased in ovx rats fed soybeans and fermented soybeans. CONCLUSIONS: Supplementation of fermented soybeans could have preventive and therapeutic effects against osteoporosis in postmenopausal women.

Reversal of dexamethasone suppressed wound healing with ethanolic extract of leaves of hyptis suaveolens (L) poit.

The dexamethasone suppressed and normal wound healing effect of ethanolic extract of leaves of Hyptis suaveolens (L) poit. (EEHS) at 400mg/kg and 800mg/kg was studied in Wistar rats. Two wound models viz. incision and excision wounds were used in this study. The parameters studied were breaking strength in case of incision wounds, period of epithelization and wound contraction in case of excision wound. The dexamethasone treated group showed a significant (P<0.05) reduction in the wound breaking strength in incision type of wound model and significant increase in epithelization period and reduction in percentage of wound contraction in excision type of wound model as compared to control group. Extract treated groups showed significant (P<0.05) improvement in all the wound healing parameters of incision and excision wound models as compared to control. In incision wound model, Co administration of EEHS at both the dosage levels with dexamethasone had significantly (P<0.05) increased the wound breaking strength as compared to dexamethasone only treated group and was seen more at dosage level of 400mg/kg. Similarly, in excision wound model, there was significant improvement (P<0.05) in both the parameters when EEHS was co administered with dexamethasone as compared to dexamethasone only treated group but improvement was more at dosage level of 800mg/kg.

Total body γ-irradiation decreases wound breaking strength during wound healing in rats / 第二军医大学学报

Objective: To investigate the influence of total body irradiation with different doses of 60Coγ on wound breaking strength during wound healing in rats. Methods: Rats were exposed to 60Coγ radiation at dosages of 4,6 ,and 8 Gy. Within 30 min after irradiation, full thickness skin wounds were made on the shaved back of rats to establish animal models of irradiated-trauma injury plus skin wounds (n=20), and non-irradiated rats with pure incision injury were used as controls (n=10). The rats were sacrificed at day 10 after treatment, and the full thickness skin wounds were harvested. Bio-mechanics method and histopathology examination were used to evaluate the wound breaking strength and histological features after healing. Results: The wound breaking strength of model groups were greatly retarded with the increase of irradiation doses. Statistical results showed that on day 10 the wound breaking strength values were significantly different between 4 Gy group ([114.26±0.29] g) and control group ([117.12±1.86] g, P>0.05), and the wound breaking strength values of 6 Gy group ([91.87±1.96] g) and 8 Gy group ([55.26±2.64] g) were significantly lower than that of the control group (P<0.05). H-E staining showed that the wounds in the irradiation trauma groups had looser and disorder collagenous fiber and less fibroblast proliferation than control group. Conclusion: Radiation injury may result in delayed wound healing, and the wound breaking strength decreases with the increase of irradiation dose in model of irradiation injury plus full thickness skin wounds.

Anastomosis colónicas en una sola capa continua con poligliconato (maxon) vs. anastomosis en dos capas con catgut crómico y seda: Estudio experimental / Single layer colonic anastomoses using maxon vs. two layer anastomoses using chromic catgut and silk: structured abstract

Background. The safety of an intestinal anastomosis is usually measured by its complication rate, especially the incidence of anastomotic leakage. A wide variety of methods have been described to reestablish intestinal continuity including single-layer continuous or two-layer interrupted anastomosis. Objectives. To evaluate if the single-layer continuous anastomosis using polygluconate is safer and reliable than two-layer interrupted anastomosis with chromic catgut and silk. Material and methods. A prospective, experimental, randomized and comparative analysis was conducted in 20 dogs. They were divided in two groups; group 1 underwent two-layer interrupted anastomosis and group 2 underwent sigle-layer continuous technique. Anastomoses were timed. Both groups were under observation. Anastomotic leakage, and other complications were evaluated. The animals were sacrified and the anastomosis was taken out together with 10 cm of colon on both sides of the anastomosis. Breaking strength, histologic evaluation and hydroxyproline determination were performed. Results. Ten two-layer anastomosis and ten single-layer anastomosis were performed. A median of 25 minutes (range: 20-30 minutes) was required to construct the anastomoses in group 1 versus 20 minutes (range: 12-25 minutes) in group 2. All animals survived and no leakage was observed. Wound infection ocurred in four dogs (20%). Median breaking strength was 230 mm Hg in group 1 and 210 mm Hg in group 2. Hydroxyproline concentration was 8.94 mg/g in group 1 (range: 5.33-16.71) and 9.94 mg/g in group 2 (range: 2.96-21.87). There was no difference among groups about the inflammatory response evaluated by pathology. There was no statistical significance in any variable evaluated. Conclusions. This study demonstrates that a single-layer continuous is similar in terms of safety to the two-layer technique, but because of its facility to perform, the single-layer technique could be superior.

Antecedentes. Una de las preocupaciones más importantes en cirugía colorrectal es la presencia de dehiscencia o fístula de la anastomosis. Múltiples técnicas han sido descritas para realizar anastomosis, entre las que se incluyen las anastomosis en una sola capa o en dos capas. Objetivos. Evaluar si la anastomosis colonica en una capa con poligliconato es más segura y efectiva que la anastomosis colonica en dos capas con catgut crómico y seda. Material y métodos. Se llevó a cabo un estudio prospectivo, experimental, aleatorio y comparativo en 20 perros. Se dividieron a los animales en dos grupos; grupo 1: anastomosis en dos capas y grupo 2: anastomosis en una sola capa. Se evaluó el tiempo de duración de la anastomosis. Todos los animales se mantuvieron en observación evaluando datos de complicaciones como fístulas colocutáneas o dehiscencia de la anastomosis. En el día diez del postoperatorio se sacrificaron. Se resecó el segmento de la anastomosis abarcando 10 cm proximales y 10 cm distales. Se midió la presión de ruptura, se realizó análisis histopatológico y se determinó la cantidad de hidroxiprolina de la línea de la anastomosis. Resultados. Se incluyeron diez perros en el grupo 1 y diez perros en el grupo 2. La mediana del tiempo de duración de la anastomosis en el grupo 1 fue de 25 minutos (rango: 20-30 minutos) y en el grupo 2 de 20 minutos (rango: 12-25 minutos) (p = NS). No se presentaron datos de fístula, dehiscencia o estenosis de la anastomosis. Cuatro perros presentaron infección en la herida. La presión de ruptura del grupo 1 fue de 230 mm Hg (115-360) y del grupo 2 fue de 210 mm Hg (100-300). La concentración de hidroxiprolina en el grupo 1 fue de 8.94 mg/gramo (rango: 5.33-16.71), y en el grupo 2 fue de 9.94 mg/gramo (rango: 2.96-21.87). No se encontró diferencia significativa en las variables analizadas. Se comparó el grado de reacción inflamatoria en ambos grupos, no hubo diferencia estadística. Conclusiones. Los dos procedimientos son seguros y confiables de realizar, aunque por su mayor facilidad, estos datos apoyan la utilización del método de una sola capa.

The Effect of Water Soluble Chitin on Wound Healing in Rats

The effect of water soluble chitin on wound healing in rats Chitin and chitosan, polysaccharides made up of polymeric N-acetyl glucosamine and D-glucosamine, are widely found in nature, forming skeletons of crustaceans and insects as well as components of bacteria cell walls. Chitin and chitosan have been known to have many useful biological properties such as biocompatibility, biodegradability, antimicrobial activity, and wound healing property. The key-point of practical use of chitin was making water soluble form. The purpose of this study was to compare the wound healing effect of water soluble with insoluble chitin. Full-thickness skin incision was made on the backs of the Sprague-Dawley rats. Three groups composed respectively with saline, water insoluble chitin and water soluble chitin were embedded in the wounds. The wound breaking strength and the collagen-hydroxyproline content of the wounded skins were measured and histological examination was performed at the time of 3, 7, 10 days respectively after the procedure. The tensile strength was maximum in case of water soluble chitin. The amount of collagen hydroxyproline was minimum in water soluble chitin. The wound treated with the water soluble and insoluble chitin was completely reepithelialized and granulation tissue in the wound was observed at 7 days after initial wounding. The arrangement of collagen fibers in the skin was orderly and similar to normal skin.

Assessment of mechanical properties of three silicone elastomers / 实用口腔医学杂志

0.05);the elongation (%) 470?9.5,560?8.2 and 470?5.8 (SY-1 versus SY-2 or MDX-4-4210,P

EFFECTS OF ZINC ON COLLAGEN SYNTHESIS AND WOUND HEALING IN RATS / 营养学报

The effects of zinc on collagen synthesis and wound healing were observed in 124 rats. The animals were divided into three groups. ZD--zincdeficient, SP--pair fed with zinc supplemented and SF---ad lib withzinc supplemented, each receiving 0.07, 0.82 and 0.84 mg Zn per rat per day respectively. An 8 cm long surgical incision was made on one side of the back of each animal and 3 pieces of sponge were implanted subcutaneous