Comparative studies of contents of alkaline substances from different prescription of formulations of Chinese material medica with same dose of aconite / 中草药

Objective: To compare with the contents of alkaline substances from four different prescription of formulations of traditional Chinese medicine (single aconite decoction, Jiangfu Decoction, Sini Decoction, and Shenfu Injection) with the same dose of aconite. Methods: Taken aconitine as the standard substance, the area under absorbance-wavelength curve (AUAWC) and absorption values of alkaline substances from four formulations, namely single aconite decoction, Jiangfu Decoction, Sini Decoction and Shenfu Injection (every concentration of aconite crude herb for 0.2 g/mL as the same dose), were obtained, respectively, which were determined by the method of AUAWC after the scanning for the wavelength from 200~600 nm and the method of bromocresol green colorimetric at 415 nm, and the data calculated were compared. Results: The results are accurate and reliable, and the methods of both AUAWC and bromocresol green colorimetric are feasible. The linear equations were Y = 0.145 42 X + 3.240 4, r = 0.999 3 for the AUAWC and Y = 0.008 63 X + 0.006 57, r = 0.999 5 for the bromocresol green colorimetric, respectively. The contents of alkaline substances from single aconite decoction, Jiangfu Decoction, Sini Decoction and Shenfu Injection were acquired through the method of AUAWC, which were 1 137.992, 2 907.120, 2 455.611, and 298.208 μg/mL, respectively, and the contents of aconitum alkaline substances were obtained by bromocresol green colorimetric with the concentration of 293.824, 447.767, 343.804 and 38.382 μg/mL, respectively. The content determined by the former method was much larger than that of the latter method, but the trend of the contents from two methods was the same, which was Jiangfu Decoction > Sini Decoction > single aconite decoction > Shenfu Injection. Conclusion: The contents of alkaline substances from different prescription of formulations of traditional Chinese medicine with the same dose of aconite are different, and the concentration of overall alkaline substances from AUAWC is more comprehensive than that of aconitum alkaline substances determined by bromocresol green colorimetric, which will provide more scientific theory interpretation for compatibility mechanism and dose-effect relationship as well as security research of Chinese medicine preparation of aconite.

A new spectrophotometric method for the determination of fluvoxamine maleate in pure form and in pharmaceutical formulation.

A simple, accurate and highly sensitive spectrophotometric methods are proposed for the rapid and accurate determination of fluvoxamine maleate (FXA) using bromocressol green (BCG), methyl orange (MO) and bromothymol blue (BTB). The developed methods involve formation of stable yellow colored chloroform extractable ion-associate complexes of the amino derivative (basic nitrogen) of the FXA with three sulphonphthalein acid dyes, namely; BCG, MO and BTB, in potassium hydrogen phthalate buffer pH 3.3, 3.6 and 3.4 respectively. The ion-associates exhibit absorption maxima at 420, 420 and 410 nm for BCG, MO and BTB, respectively. FXA can be determined up to 2.0–16, 2.0–15 and 2.0–20 μgmL−1 for BCG, MO and BTB, respectively. The effect of optimum conditions via pH on the ion pair formation, reagent concentration, time and temperature, and solvent was studied. The composition of the ion pairs was found 1:1 by Job’s method. The low relative standard deviation values indicate good precision and high recovery values. These methods have been successfully applied for the assay of FXA in pure form and in pharmaceutical formulations and the results are in good agreement with those obtained by the official method.

Extractive colorimetric method development and validation for erlotinib in bulk and tablet dosage form.

Two simple, rapid, sensitive, precise and economic spectrophotometric methods have been developed for the estimation of Erlotinib in bulk and tablet formulation. During the course of study, it was observed that solution of the drug formed colored ion-pair complexes with Bromocresol Green (BCG) and Methyl Orange (MO) in phosphate buffer pH 2.5, and extracted in chloroform. This property of the drug was followed for the development of colorimetric methods for analysis of drug. The complex of etoricoxib with BCG and MO showed λ max at 418.5 nm and 424.4 nm respectively. The complex was stable up to 22 hrs and obeyed Beer's law over the concentration ranges of 10-1000 μg/ml. Correlation coefficient was found to be 0.9985. In addition we have determined the molar absorptivity, Sandell sensitivity and the optimum conditions for quantitative analysis of erlotinib. These methods were validated statistically. Recovery studies gave satisfactory results indicating that none of common additives and excipients interfere the assay method. The proposed methods are found to be simple, accurate and reproducible that was successfully applied for the analysis of tablet formulation.

Determination of Alkaloids in pericarpium papaveris by visible spectrophotography / 中成药

Objective: To establish the determination of alkaloids in Pericarpium Papaveris. Method: Adopting the bromocresol green as colored indicator, alkaloids in Pericarpium Papaveris were measured by visible spectrophotography. Results: The average recovery was 101.2%, the RSD was 2.46%.Conclusion: There are big difference in batchs, we should enhance the quality control of Pericarpium Papaveris.