RESUMO
Introduction: Anastrozole is an anti-cancer drug, an effective aromatase inhibitor for the treatment of breast cancerin post-menopausal women. As it undergoes extensive first-pass metabolism and has many side effects related to oraluse, it has been envisaged to develop anastrozole invasomes in the form of transdermal gel.Objective: The objective of this work was to prepare, characterize, and evaluate invasomal gel of anastrozole.Materials and Methods: Invasomes were prepared by thin layer film hydration method using Phospholipon 80H,fenchone (terpene), and ethanol. The optimized invasomes were incorporated into sodium carboxy methyl cellulosegel. Prepared formulations were evaluated and cytotoxic study on Michigan cancer foundation (MCF)-7 cancer cellline was studied.Results and Discussion: The scanning electron microscope results of the optimized formulation showed sphericalshaped vesicles.The ex vivo permeation of invasomes and the skin deposition (73%) were studied on male Wistar ratskin. Cell line studies on MCF-7 cells showed cytotoxic effect of optimized formulation at 5 µl/ml.Conclusion: It was concluded that the developed anastrozole invasomes enhanced the transdermal flux and theresults obtained encouraged the use of the anastrozole invasomal gel for the potential treatment of breast cancer inpost-menopausal women.
RESUMO
Introduction: Anastrozole is an anti-cancer drug, an effective aromatase inhibitor for the treatment of breast cancerin post-menopausal women. As it undergoes extensive first-pass metabolism and has many side effects related to oraluse, it has been envisaged to develop anastrozole invasomes in the form of transdermal gel.Objective: The objective of this work was to prepare, characterize, and evaluate invasomal gel of anastrozole.Materials and Methods: Invasomes were prepared by thin layer film hydration method using Phospholipon 80H,fenchone (terpene), and ethanol. The optimized invasomes were incorporated into sodium carboxy methyl cellulosegel. Prepared formulations were evaluated and cytotoxic study on Michigan cancer foundation (MCF)-7 cancer cellline was studied.Results and Discussion: The scanning electron microscope results of the optimized formulation showed sphericalshaped vesicles.The ex vivo permeation of invasomes and the skin deposition (73%) were studied on male Wistar ratskin. Cell line studies on MCF-7 cells showed cytotoxic effect of optimized formulation at 5 µl/ml.Conclusion: It was concluded that the developed anastrozole invasomes enhanced the transdermal flux and theresults obtained encouraged the use of the anastrozole invasomal gel for the potential treatment of breast cancer inpost-menopausal women
RESUMO
ABSTRACT Human have been constantly using plants and plant products to overcome many diseases. The antioxidant property of the plant sources is studied to obtain an efficacious drug against cancer. The objectives of the present study is to evaluate the antioxidant and cytotoxic activity of the Tecoma stans extracts against lung cancer cell line in comparison with vincristine drug. The antioxidant activity was studied using the standard DPPH assay and the cytotoxic activity using MTT assay. DPPH assay results show that methanolic extract of T. stans in higher concentration show better antioxidant potential than the standard L-ascorbic acid. They exhibited strong antioxidant potential at 20 µg/mL concentration. The absorbance at 517 nm showed that in the range of 0.201-0.0203 compared to that of absorbance of ascorbic acid at 0.023.Cytotoxic activity was studied using MTT assay which showed that the increase in concentration of extract increases the cell death. At 100µg/mL concentration there is an increased cytotoxic activity, i.e., 99% of cell inhibition. The results of antioxidant and anticancerous activity may be positively correlated.