Determination of entrapment efficiency of bulleyaconitine A multivesicular liposomes / 中成药

AIM:To establish a method of determining the entrapment efficiency of bulleyaconitine A multivesicular liposomes.METHODS:Bulleyaconitine multivesicular liposomes and its free bulleyaconitine were separated by centrifugalization.To calculate the entrapment efficiency,total and free bulleyaconitine were assayed by HPLC.RESULTS:At low speed,the rate of rotation did not affect the determination of entrapment efficiency.The average entrapment efficiency of three lot samples was 87.12%.CONCLUSION:Entrapment efficiency of bulleyaconitine multivesicular liposomes could be evaluated simply and quickly by centrifugalization.