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Objective To investigate the changes of serum endotoxin and its receptor CD 14 gene expression in multiple organs in models of acute forebrain ischemia complicated with multiple organ dysfunction syndrome (MODS), and the pathogenesis of cerebrogenic multiple organ dysfunction syndrome (CMODS). Methods 54 Wistar rats were randomly divided into normal control group ( n=6), sham-operative group ( n=8) and forebrain ischemic group ( n=40). The rats in forebrain ischemic group were randomly divided into 5 subgroups: 12, 24, 36, 48, and 72 h (8 rats in each group). The contents of endotoxin in plasma were determined after models of acute forebrain infarction established. The area density and optical density of positive staining expressing CD 14mRNA in lung, liver, intestine and kidney were analyzed for the relative content of CD 14mRNA using in situ hybridization and CMIA medical image analysis system.Results Plasma endotoxin level was markedly high at 12 hours after acute forebrain ischemia, peaked at 24 hours and somewhat decreased at 72 hours. The CD 14mRNA expression in lung, liver, intestine, and kidney tissues increased after brain ischemia, reached the peak at 24~36 h, and decreased after 48 hours. The highest change of CD 14mRNA expression was found in lung ( P
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Objective To explore the effects of the vagus and its transmitter on cerebrogenic multiple organ dysfunction syndrome induced by cerebral hemorrhage(CH) in rats. Methods 80 wistar rats were randomly divided into sham-operative group (n=10),subdiaphringmatic vagotomy (SDV) group (n=10),CH group (n=20), CH+SDV group (n=20) and CH+SIV group (n=20).Rat CH model was made by stereotaxis injection of collagenase and heparin sodium in caudate nucleus. The success full CMODS rat model was characterized by the abnormality of hepatic and renal function,myocardium enzyme, blood routine and vital sign of rat chalked standard. The rats of CH+SDV group were practiced SDV operation 4 weeks before the model was made. CH+SIV group were put electrostimulation of vagus after the mode was made for 6 times. The stimulus continued 20 min with 4 h interval. All rats were executed 24 h after CH. The pathological change of main organs (liver, kidney, lung, small intestine),blood routine were tested. Myocardium enzyme,hepatic and renal function were examinied. Meanwhile, the expression of FOS,CHAT and VIP in the medullary visceral zone of the rats were examined with immunohistochemistry,compared with the sham-operative group and SOV gruop. Results Abnormality of hepatic and renal function,myocardium enzyme and the pathological change of liver,kidney,small intestine CH+SDV group,CH+SIV group and those of CH group outweigh than sham-operative group and SDV group(all P