RESUMO
Objective: To study the change regulation of nine compounds(oxypaeoniflorin, albiflorin, paeoniflorin, benzoyl-paeoniflorin, glycyrrhizic acid, liquiritin, isoliquiritin, liquiritigenin, and isoliquiritigenin) in Peony and Licorice decoction, and analyze the correlation between therapeutical effects and active components alignment. Methods: Classical prescription Peony and Licorice decoction (Shanghanlun) were taken as the carrier, five different combinations of Peony and Licorice decoction were used as the control, and the content was determined by HPLC-DAD. Results: The contents of nine compounds of different combinations of Peony and Licorice decoction was(mg/ml): Shanghanlun 1.92, 0.90, 3.51, 0.53, 8.35, 3.16, 0.77, 0.77 and 0.15; Chuanxinshiyongfang 0.92, 0.45, 1.62, 0.37, 0.78, 0.34, 0.06, 0.10 and 0.01, Zhushijiyanfang A 1.59, 0.82, 3.28, 0.49, 3.15, 1.10, 0.27, 0.44 and 0.08, Zhushijiyan-fang B 1.14, 0.52, 2.12, 0.41, 2.92, 0.97, 0.22, 0.36 and 0.06, Weishijiacangfang 1.38, 0.59, 2.70, 0.43, 2.40, 0.35, 0.06, 0.10 and 0.01, Yi-menbafang 1.52, 0.67, 2.91, 0.45, 3.00, 1.07, 0.24, 0.42 and 0.07. Conclusion: 1. The change regulation of the nice compounds was Shang-hanlun>Zhushi-jiyanfang A > Yimenbafang, oxypaeoniflorin, albiflorin, paeoniflorin and benzoylpaeoniflorin in Zhushijiyanfang B Weishijia cangfang. chuanxinshiy-ongfang has the the lowest content of the nine compounds. 2. Total contents and proportion of the nine compounds are different due to different compatibility. The clinical curative effect is also different. Total contents of the nine compounds in Shanghanlun were 20.06 mg/ml which is used to relieve pain in the legs, in Zhushijiyanfang A was 8.72 mg/ml which is used to relieve pain caused by diabetes, in Zhushijiyanfang B was 8.72 mg/ml which is used to treat foot weakness, in Chuanxinshiyong-fang was 4.65 mg/ml which is used to treat wet beriberi, in Weishijiacangfang was 8.02 mg/ml which is used to treat wet beriberi, in Yimenbafang was 10.35 mg/ml which is used to treat peratodynia.
RESUMO
Objective To explore the change regulation of blood coagulation function before and after treatment of snake bite pa‐tients .Methods A total of 226 hospitalized patients with clear diagnosis belong to what kind of snakes in the past two years were selected in this study ,39 cases were bit by coral ,76 cases were bit by trimeresurus stejnegeri ,47 cases were bit by cobra ,24 cases bit by king cobra ,40 cases were bit by adder .Five blood coagulation indexes were determined before and after treatment various pe‐riods for these patients .The tested results were made to statistical analysis according to kind of snakes ,periods and disease condi‐tion .Results D‐D level obvious .rise before treatment for the severe cases patients of coral snake bite (P0 .05) before and after treatment ,only D‐D level of the severe cases had statistical significance before treat‐ment in contrast to the mild cases of cobra bite (P< 0 .05) .The changes of 5 item blood coagulation indexes were all very large whether mild or severe for viper bite patients ,a number of comparisons had statistical significance difference (P<0 .05) whether before or after treatment and whether mild or severe for viper bite patients .Conclusion The bite of coral snake ,cobra and king co‐bra affect little for the blood coagulation function .The severe cases patients of trimeresurus stejnegeri bite might lead to more seri‐ous blood coagulation function abnormality but the recovery is faster after treatment .The viper bite might lead to maximum blood coagulation function abnormality and the recovery is slow after treatment .