Preliminary detection of alanine aminotransferase before blood donation / 中国输血杂志

【Objective】 To explore the optimized strategy of alanine aminotransferase(ALT) screening before blood donation, in order to reduce the waste of blood resources. 【Methods】 The blood donation scrapping of one blood center in Chongqing from January to April 2020 was analyzed retrospectively. The correlation between ALT test results by dry chemistry method and rate method in 500 blood donors, with ALT level close to the limit, was analyzed, and the abnormal rates of retest were stratified by initial results of ALT dry chemical method. 【Results】 Among blood donors enrolled in this study, the rate of ALT abnormality(0.89%, 43/4 846)was significantly higher than that of HBV (0.25%, 12/4 846), HCV (0.43%, 21/4 846) and HIV (0.19%, 9/4 846)(P<0.05). For the samples with pre-donation ALT close to the limit (40 U/L≤ALT≤50 U/L), a weak correlation between the results of dry chemistry method and rate method was observed, with correlation coefficient at 0.33 (P<0.05). The abnormal rates in retest were significantly higher in 45 U/L≤ ALT ≤50 U/L (10.7%, 22/206) group than that in the ALT < 45U/L group(P<0.05). 【Conclusion】 The ALT limit before blood donation should be set to 45 U/L. For blood donors with ALT within the range of (45~50) U / L, blood donation should be deferred until they passed retests by rate method.

Research progress on ossification by titanium nanotube implant modification / 口腔疾病防治

@#Surface modification of titanium implants is a hot topic for improving osteointegration and includes physical, chemical, bioactive and anodization methods. Among these methods, anodization methods can form TiO2 nanotube structures with a uniform and stable structure, and TiO2 nanotubes and substrates have high binding strengths and osteogenic properties and represent an excellent method for implant modification. TiO2 nanotube osteogenesis is closely related to its morphology, diameter and physicochemical characteristics. Therefore, the structure of TiO2 nanotubes with optimal osteogenic performance can be prepared by regulating these factors. At present, research on TiO2 nanotubes is mostly focused on composite treatments with TiO2 nanotubes, namely, the combination of other implant modification methods (physical method, chemical method, biological method) and TiO2 nanotubes to form a composite structure to work synergistically to treat osteogenesis. TiO2 nanotube composite treatment is a good prospective application for the further preparation of TiO2 nanotube-modified structures with strong osteogenic properties.

Research progress on color determination methods of reagent strips in routine urinalysis / 国际生物医学工程杂志

Clinically, routine urinalysis includes more than 10 items, such as urine protein, urinary pH, urinary bilirubin and so on. The above items are usually analyzed by dry chemical methods. The principle is that after the urine infiltrates the reagent strips, the color changes can be observed in each strip. The color depth is related to the component concentration, and the darker the color, the greater the measured component concentration, and vice versa. In this paper, four color discrimination methods in urinary dry chemical analysis were introduced, i.e. the method based on integrating sphere photoelectric conversion, the method based on charge-coupled device (CCD) image sensor, the method based on color sensor, and the method based on the combination of color sensor and fiber bundle. Besides, the four methods were compared and analyzed. Finally, the recent research progress in urinalysis was reviewed.

Performance verification of VITROS 5600 automatic biochemical analyzer / 国际检验医学杂志

Objective To verify the performance of VITROS 5600 automatic biochemical analyzer.Methods According to laboratory accreditation criteria and performance verification documents of American CLIA'88,the precision,accuracy,liner range,clinical reportable range and biological reference interval were verified for the test of 17 conventional biochemical indicators performed on VITROS 5600 automatic biochemical analyzer.Results The precision、accuracy、liner range、clinical reportable range and biological reference interval of VITROS 5600 automatic biochemical analyzer were all acceptable.Conclusion VITROS 5600 automatic biochemical analyzer could fully meet the requirements in clinical application.

Influence of injection cefotiam on vanadate oxidation method and dry chemical method assay total bilirubin / 国际检验医学杂志

Objective Discuss the interference of injection cefotiam on vanadate oxidation method and dry chemical method assay total bilirubin .Methods Collected 60 examples ,include total bilirubin concentration 20 examples less than 20 μmol/L ,20 examples between 150-220 μmol/L and 20 examples between 350-410 μmol/L ,add an equal volume of various concentrations of cefotiam in each case ,formulated into cefotiam final concentrations of 300 ,150 ,75 mg/L of serum samples as the test group ,add an equal volume of water in each serum samples as the control group ,determine all the samples total bilirubin concentration respectively by vanadate oxidation method and dry chemical method ,compared the interference of cefotiam on determined total bilirubin by two method ,analyze the data by SPSS13 .0 .Results Determined total bilirubin by dry chemical method ,the test group higher than the control group ,the difference was statistically significant(P<0 .05) ,at the same total bilirubin levels ,with cefotiam concentrations decreased ,increased rate of total bilirubin concentration were decreased in the experimental group .Determined total bilirubin by vanadate oxidation method ,when the total bilirubin concentration between 150 -220 μmol/L ,the test group was higher than the control group ,the difference was statistically significant(P<0 .05) .Conclusion Interference of injection cefotiam on determined to‐tal bilirubin by dry chemical method is strong ,and with the drug concentration increased ,effect is more obvious ,but determination of total bilirubin by vanadate oxidation method has almost no effect .

Analysis of factors affecting results of ALT preliminary screening in blood donors / 国际检验医学杂志

Objective To explore the factors affecting results of alanine aminotransferase(ALT)preliminary screening by using dry chemical method in blood donors,so as to establish standards of ALT preliminary screening and reduce waste of blood. Methods A total of 21 065 blood specimen of blood donors in Blood Center of Maoming City were collected from January to June 2013.The influences of specimen hemolysis,lipidemia,different sample amount and waiting time after adding sample on the detec-tion results of ALT by using dry chemical method were comparatively analysed,and standardized procedures and established appro-priate limits of ALT preliminary screening according to the results.Results The specimen hemolysis affected test results of ALT, which was not obvious when the hemolysis rate was less than or equal to 0.5%.There was no obvious interference of lipidemia on ALT test.When the sample amount was (32±3)μL or the waiting time after adding sample was 10 to 40 seconds,the detection re-sults of ALT were acceptable.The standard limit of ALT was less than or equal to 40 U/L of female and less than or equal to 45 U/L of male.Conclusion Hemolysis,sample amount and waiting time after adding sample can influence the results of ALT detec-ted by using dry chemical method,while lipidemia had no interference on ALT test.Standardizing operation and reasonably setting normal limits standard may ensure the accuracy and stability of determination,save blood resources,further ensure blood safety, which should be worthy of wide application.

Effects of Nano-sized Calcium on Intestinal Absorption and Bone Turnover / 대한골다공증학회지

OBJECTIVES: We compared the effects of two different nano-sized calcium supplements (synthesized by wet-chemical method or the dry-grinding method of calcium carbonate) and one micro-sized calcium supplement (calcium carbonate) on intestinal absorption and bone turnover in 20 young women. MATERIALS & METHODS: This study was carried out with a three week cross-over design. The subjects ingested one of three different calcium supplements (1 dose of 500 mg Ca++) at 8 AM, which was repeated three times weekly. Serum and urine samples were collected before and after the calcium load using a time table (serum sampling 0, 2 hr, 4 hr, 8 hr after loading; urine sampling 0, 4 hr, 8 hr after loading). RESULTS: Serum ionized calcium and parathyroid hormone concentrations significantly changed in response to all calcium supplements loading, the difference among calcium supplements was not significant. However, the postload urinary excretion of calcium and the N-telopeptide/creatinine ratio were significantly different among three calcium supplement over time by repeated measured ANOVA. CONCLUSIONS: The results of this study suggest the effect of nano-sized calcium supplements synthesized by the wet-chemical method is superior to the other calcium supplements in intestinal calcium absorption and bone turn-over.

Analysis of the Urinary Stone Components Using Chemical Analysis Method / 대한비뇨기과학회지

We analyzed the composition of urinary stones using chemical analysis method. From 1985 to 1995, 495 urinary calculi were eligible for stone analysis. Forty six per cent of the stones were composed of single chemical component, and pure calcium oxalate stone was the most common type of the stones consisting 34.9% of all stones, followed by 23.8% of mixture of calcium oxalate and calcium phosphate, and 92.7% of the stones had calcium component. Calcium oxalate crystal was found in 82.6% of the stones, calcium phosphate in 50.5%, ammonium magnesium phosphate in 26.7%, uric acid in 11.3% and cystine in 0.4%. Nation-wide extension of this study using chemical analysis will be mandatory for a proper assessment of the stone composition of Koreans and for planning of the proper management strategies for urinary stones in Korea.