Effects of edaravone on learning and memory abilities and expression of choline acetyl transferase and high affinity choline transporter in vascular dementia rats / 中华行为医学与脑科学杂志

Objective To investigate the effect and mechanism of edaravone on learning and memory abilities and expressing of choline acetyl transferase(ChaT) and high affinity choline transporter(ChT) in vascular dementia (VaD) rats.Methods 30 rats were randomly divided into sham operation group,model group and edaravone treatment group.The sham operation group with bilateral carotid arteries were isolated only,but did not block the blood flow and not inject drugs.VaD model was established by two-vessel method in model group and edaravone treatment group.The three groups underwent the Morris water maze test and immunohistochemistry to detect the expression of ChaT and ChT in hippocampus.Conclusion The first day,Morris water maze test showed that the model group escape latency ((92.6 ± 8.1) s) was significantly greater than the sham operation group ((71.9 ± 5.1) s) and edaravone treatment group ((71.2 ± 4.9) s),the differences were statistically significant (P ＜0.05).ChaT hippocampal gray value in the model group((89.05 ±9.42)) was higher than that in sham operation group(67.44 ± 6.36) and edaravone treatment group(62.35 ± 4.69),the differences were statistically significant (P ＜ 0.05).ChT hippocampal gray value in the model group(145.33 ± 3.79) was lower than that in sham operation group (164.21 ± 2.85) and edaravone treatment group (162.45 ± 4.72),the differences were statistically significant (P ＜ 0.05).Results Edaravone may be effective in improving learning and memory and protecting neurons in rats,and improve the expression of ChaT,but decrease the expression of ChT in hippocampal.

Effect of Nasal Administration of Compound of Cholera Toxin B Subunit and Nerve Growth Factor on Spatial Learning and Memory Abilities in the Amnesic Mice / 中国康复理论与实践

@#ObjectiveTo explore the effects of the conjugate prepared from the cholera toxin B subunit(CB) and nerve growth factor(NGF) on the spatial learning and memory abilities and cholinergic function.MethodsThe conjugate of CB-NGF was prepared by the improved sodium metaperiodate method and nasally administrated to the β-amyloid protein(Aβ25-35) induced amnesic mice for 7 days with 2 dosage (7-5 μg/d、15 μg/d). Spatial learning and memory abilities were evaluated by Morris water maze and cholinergic function was assessed with the choline acetyl transferase (ChAT) immunohistochemical methods.ResultsMorris water maze test showed that the escape latency in Aβ25-35-treated mice prolonged and the staying time reduced in the crossed first quadrant where the platform had been located, compared with the control mice (P<0-01). In addition, the number of ChAT positive neuron declined in the model mice(P<0-001). CB-NGF nasal administration significantly shortened the escape latency and elevated the staying time and number of ChAT positive neuron(P<0-01).ConclusionCB-NGF treatment can improve the spatial and memory performance which may involve the neuroprotection to cholinergic system.

The effect of ginkgolides on expression of ChAT in the Alzheimers disease-like rat brain / 中国药理学通报

Aim To investgate the mechnism through which ginkgolides affect learning and memory capabilities of the Alzheimers disease-like rats. Methods Okadaic acid(OA)was injected into the CA1 region of the rat hippocampus and the rats were gavaged with ginkgolides. The learning and memory abilities of the rats were assessed through Morris water maze behavioral test, and the expressions of nicotinic acetylcholine receptors and ChAT were observed by Western blotting and immunohistochemistry, respectively.Results Compared with the control rats, the capabilities of learning and memory were lowered significantly(P