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Objective To observe the protective effects of genistein sodium sulfonate(GSS)on mice chronic hepatic injury in-duced by carbon tetrachloride(CCl4 )and its influence on the protein expression of α7 nicotinic acetylcholine receptor(α7nAChR) and interleukin-1 beta (IL-1β)in liver tissue.Methods 60 SPF grade male Kunming mice were randomly divided into 5 groups,in-cluding the control group,model group,low and high doses GSS groups,and positive control group,12 cases in each group.Except for the control group,the other 4 groups were intra peritoneally injected by 10 % CCl4 with a volume of 0.1 mL/10 g for 6 weeks. The mice chronic liver injury was prepared.At the same time,the high and low doses DSS groups were given the different doses of GSS(0.30,0.10 mg/kg),the positive control group was given bifendate(DDB,2.5mg/kg),the control group and the model group were given the equal volume of normal saline for 6 consecutive weeks.The AST and ALT activity was detect and the ratio of ALT/AST was calculated;the Western blot method was used to detect the expression levels ofα7nAChR and IL-1βprotein in liver.Re-sults The serum levels of ALT and AST in the model group were increased obviously,and the expression level ofα7nAChR in the liver tissue was decreased,while the expression level of IL-1βwas increased;after the GSS treatment,the serum AST and ALT lev-els were significantly lower than those in the model group(P <0.05),while the expression level ofα7nAChR was increased (P <0.01)and the expression level of IL-1βwas decreased(P <0.05).Conclusion GSS might increase the expression ofα7nAChR in injured liver tissue,activates the cholinergic anti-inflammatory pathway,thus decreases the expression of inflammatory cytokines and antagonizes the mice chronic liver injury by inhibiting the inflammatory reaction.
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Objective To study the therapeutic effect and mechanism of Jiawei Xiaochengqi Decoction on hepatic injures by endotoxin in rats. Methods Endotoxemia model of Wistar rats was made by thioacetamide. Blood biochemistry and pathological changes were examined to observe the effect of Jiawei Xiaochengqi decoction on liver function, TNF-?, endotoxin of the model rats. Results Jiawei Xiaochengqi Decoction could reduce the contents of TNF-?, endotoxin in the blood and the degree of hepatic cells necrosis of Wistar rats. There was significant difference compared with model group (P
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During the period of the subcutaneous injection of CCl_4 0.5ml/kg, four times at intervals of 5 days, the C57 mice were fed with 6.8％ bee pollen of Codonopsis Pilosula Nannf (BPCPN). The results showed that BPCPN coule alleviate effctively the hepatic injury induced by CCl_4, and prevent the decrease of serum albumin and liver protein and glycogen after the liver was injured. The electron microscopis and pathologcal examination also showed that the mice had only slight hepatic necrosis, fat degeneration and fibrosis, but had a com plete repair as compared with the control groups. The above results indicated that BPCPN possessed preventive and curative on the impairment of CCl_4and could protect the hepatic metabolic function from the injury of CCl_4.