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Aims Tocomparethepharmacokineticsof ephedra alkaloids,cinnamic acid and cinnamic alcohol in Ephedrae, Cinnamomi and Ephedrae-Cinnamomi herb couple through UPLC-MS/MS in rats respective-ly,and to investigate the effect of combination on phys-iologicaldisposition.Methods Plasmasampleswere collected at different times after oral administration of Ephedrae,Cinnamomi and Ephedrae-Cinnamomi herb couple extracts.The concentrations of ephedra alka-loids,cinnamic acid and cinnamic alcohol in plasma samples were determined by UPLC-MS/MS.DAS 3. 0 was used to calculate pharmacokinetic parameters.The differences of samples in two groups were conducted with univariate statistical analysis using SPSS 13. 0. Results ComparedwithEphedraegroup,theCmaxof norephedrine hydrochloride, norpseudoephedrine hydrochloride,ephedrine hydrochloride,pseudoephed-rine hydrochloride,and methylephedrine hydrochloride in Ephedrae-Cinnamomi herb couple group were signif-icantly greater (P<0. 05 );the AUC0-t of norpseudo-ephedrine hydrochloride was significantly greater (P<0. 05 );the MRT0-t of norephedrine hydrochloride, phedrine hydrochloride, pseudoephedrine hydrochlo-ride,and methylephedrine hydrochloride were signifi-cantly less (P <0. 05 );the T1/2z of norephedrine hydrochloride,phedrine hydrochloride,and methyl-ephedrine hydrochloride were significantly less (P <0. 05 ).The AUC0-t and MRT0-t of cinnamic acid and cinnamic alcohol were significantly greater than those inCinnamomigroup(P<0.05).Conclusion The combination of Ephedrae and Cinnamomi improves the absorption concentration of five ephedra alkaloids, Slows down the elimination of norephedrine hydrochlo-ride,phedrine hydrochloride,pseudoephedrine hydro-chloride,and methylephedrine hydrochloride,and in-creases the bioavailability of cinnamic acid and cin-namic alcohol.
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Objective: To investigate the chemical constituents from the petroleum ether and ethyl acetate extract of Guizhi Fuling Capsule (GFC). Methods: The compounds were separated with chromatography on silica gel and polydextran gel columns, and prep-HPLC, and their chemical structures were identified by NMR and MS spectral methods, respectively. Results: Eight compounds were isolated from the petroleun ether extract and were identified as coumarin (1), 7-hydroxycoumarin (2), cinnamic alcohol (3), ergosterol (4), ergosta-4, 6, 8 (14), 22-tetraen-3-one (5), β-amyrin acetate (6), campesterol (7), and α-amyrin acetate (8). Thirteen compounds were isolated from the ethyl acetate extract and were identified as apingenin (9), kaemferol (10), quercetin (11), kaempferol-3-O-glucopyranoside (12), catechin (13), epicatechin (14), syringic acid (15), hyperin (16), eicosanoids (17), syringaresinol (18), mudanpioside A (19), mudanpioside C (20), and galloylpaeoniflorin (21). Conclusion: Compounds 1-21 are isolated from GFC for the first time.
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The components of volatile oil in radix and rhizome of Changbaishan Rhodiola Sachliensis A. Bor. were analysed with combine use method of GC-MS-Computer. A total of 26 components were separated and identified. The cinnamic alcohol content among them was highest, relative content reaching 26%. The volatile oil contained 7 sesquiterpene components. The content of ?-caryophyllene and ?—patchoulene was higher among them, 12% and 1.86% of total volatile oil, respectively.