RESUMO
Objective To prepare cysteamine group substitutes of catechin compounds from Weimaining. Methods After thiolased by cysteamine, Weimaining was subjected to the open column chromatography on C18, and subsequently to the semipreparative high performance liquid chromatograph (HPLC) to prepare the cysteamine group substitutes of catechin compounds. Results Compounds 4β-(2-aminoethylthio)catechin, 4β-(2-aminoethylthio)epicatechin, and 4β-(2-aminoethylthio) epicatechin-3- J-gallate were obtained, and their purities were above 95%. Conclusion The method is simple and easy, and provides a reference for the preparation of the cysteamine group substitutes of catechin compounds.
RESUMO
Objective To prepare cysteamine group substitutes of catechin compounds from Weimaining. Methods After thiolased by cysteamine, Weimaining was subjected to the open column chromatography on C18, and subsequently to the semi-preparative high performance liquid chromatograph (HPLC) to prepare the cysteamine group substitutes of catechin compounds. Results Compounds 4β-(2-aminoethylthio)catechin, 4β-(2-aminoethylthio)epicatechin, and 4β-(2-aminoethylthio) epicatechin-3-O-gallate were obtained, and their purities were above 95%. Conclusion The method is simple and easy, and provides a reference for the preparation of the cysteamine group substitutes of catechin compounds.