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Mycorrhizal association is an integral part of terrestrial ecosystems. The present work was focused to examinearbuscular mycorrhiza (AM), dark septate endophyte (DSE) fungal colonization, and the composition of AMfungi from two home gardens of Tripura in Northeast India. The results reveal eight plants commonly occurringin two sites belonging to seven families. Of the eight plants, dual colonization of AM and DSE fungi wasobserved in seven plants from two sites. A total of 18 AM fungal species were recovered from both the sites.The study reveals a robust composition of AM fungi in the home garden ecosystem. AM fungi isolated fromthese ecosystems confirm their occurrence and these fungi may be beneficial in improving the cultivationpractices in the home garden systems of the region.
RESUMO
OBJECTIVE: To investigate extensively the cultural endophytic fungal community colonized mainly in the roots of five orchid medicinal plants species. METHODS: Cultural endophytic fungi were identified using the morphological methods and molecular sequences. RESULTS: A total of 241 strains of fungi were isolated and identified into at least 52 genera. Among them, Fusarium (14.9%), Colletotrichum(12.9%), Xylaria(5.4%) and Alternaria(3.7%) were dominated. Ten interested fungal strains were identified, including Dark septate endophyte (DSE) and Orchid mycorrhizal fungi(e. g. Tulasnella and Sebacina). CONCLUSION: Fungal taxa and richness are related to host plant species and its geological distribution. This study provides important fungal resources for cultivation of orchid medicinal plants and screening of bioactive compounds in the future.
RESUMO
OBJECTIVE: To examine the diversity and antimicrobial activities of the endophytic fungi of Dysosma versipellis (Hance) M. Cheng. METHODS: The identification of the fungi was carried out by rDNA-ITS sequence analysis. The antimicrobial activities of the isolated endophytic fungi were determined by agar block test. RESULTS: Nineteen strains of various endophytes were obtained from the roots, stems and leaves of D. versipellis, which were molecularly identified to belong to eight catalogues and thirteen genuses. The DV04 had remarkable antimicrobial activities to Staphylococcus aureus, Escherichia coli, and Candida albicans. Morphological and molecular identification demonstrated that DV04 represented Leptodontidium sp.. CONCLUSION: The endophytic fungi in Dysosma versipellis are diverse and rich. Some strains such as DV04 deserve further research as an alternative antimicrobial resource.