Mechanism of Bushen Yijing Method in Treatment of Oligoasthenozoospermia Based on Intestinal Microbiota / 中国实验方剂学杂志

Oligoasthenozoospermia is the main cause of male infertility， with complex and diverse causes. Currently， there are still some unclear causes of oligoasthenozoospermia in clinical practice， known as idiopathic oligoasthenozoospermia. With the development of high-throughput sequencing technology， it has been found that intestinal microbiota disorder may be an important promoting factor for the onset of oligoasthenozoospermia. Traditional Chinese medicine believes that "deficiency of kidney essence" is the core pathogenesis of oligoasthenozoospermia. In clinical practice， the method of tonifying the kidney and strengthening the essence has a significant therapeutic effect on oligoasthenozoospermia， but its mechanism of action has not been fully elucidated. Based on the basic theories of traditional Chinese medicine and molecular biology research， it has been found that there is a similarity between "kidney essence" and intestinal microbiota. During the onset of oligoasthenozoospermia， the disorder of intestinal microbiota has similarities with the pathogenesis of "deficiency of kidney essence" in traditional Chinese medicine. Moreover， traditional Chinese medicine for tonifying the kidney and strengthening the essence can regulate the disorder of intestinal microbiota， which may be one of the effective mechanisms for the treatment of oligoasthenozoospermia with the Bushen Yijing method. Based on this， this article explored the mechanism of Bushen Yijing method of traditional Chinese medicine in treating oligoasthenozoospermia from the perspective of intestinal microbiota， so as to provide new ideas for the treatment of oligoasthenozoospermia with traditional Chinese medicine.

ffects of Zuogui Pills on kidney-yin deficiency syndrome of premature ovarian insufficiency rats / 国际中医中药杂志

Objective:To study the effects of Zuogui Pills on rats with kidney-yin deficiency syndrome of premature ovarian insufficiency.Methods:Totally 40 SD female unmated rats were randomly divided into blank group, model group, Zuogui Pills group and Bujiale group, with 10 rats in each group. Except for blank group, rats in other groups were subcutaneously injected with ZP3 and gavaged with levothyroxine sodium to induce kidney-yin deficiency syndrome model of premature ovarian insufficiency. At the same time of modeling, Zuogui Pills group and Bujiale group received corresponding drugs for gavage, and the other groups received corresponding solvent for gavage, once a day, for consecutive 21 days. On day 0, 7, 14 and 21, ear temperature and body weight of rats were measured, and the ovarian index, uterus index and thyroid index were calculated. Serum levels of adenosine cyclic phosphate (cAMP), cyclic guanosine phosphate (cGMP), Cortisol, follicle-stimulating hormone (FSH), luteinizing hormone (LH) and estradiol (E 2) were detected by ELISA. Pathological changes of ovary was observed with HE staining. Results:On day 14 and 21, compared with model group, the body weight of rats in Zuogui Pills group increased ( P<0.05), and the ear temperature decreased ( P<0.05); compared with model group, the ovarian index, uterine index and thyroid index of rats in Zuogui Pills group decreased ( P<0.05), the levels of serum cAMP/cGMP, cortisol, FSH and LH decreased ( P<0.05), and the level of E 2 increased ( P<0.05). Conclusion:Zuogui Pills have certain improvement effect on rats with kidney-yin deficiency syndrome induced by levothyroxine sodium tablets combined with ZP3.

Curative effect of Wenshen-Gushu Decoction combined with conventional western medicine for the postmenopausal osteoporosis patients with kidney yang deficiency type / 国际中医中药杂志

Objective:To investigate the clinical effect of Wenshen-Gushu Decoction combined with conventional western medicine on postmenopausal osteoporosis patients with kidney yang deficiency type. Methods:A total of 105 postmenopausal osteoporosis patients admitted in Huxi Hospital Affiliated to Jining Medical College from January 2017 to December 2019 were selected. According to the random number table method, they were divided into the control group (52 cases) and observation group (53 cases). The control group was treated with conventional western medicine, and the observation group was treated with self-made Wenshen-Gushu Decoction on the basis of the control group. Both groups were treated for 8 weeks. The TCM Symptom Score, Visual Analogue Scale (VAS), bone mineral density of troch (Troch), bone mineral density of ward’s triangle (ward’s), bone mineral density of L 1-4 and serum levels of steroid hormone receptor coactivator-3 (SRC-3), B-cell lymphoma gene-2 (Bcl-2) and transcription element coactivator (PGC-1a) were compared between two groups before and after treatment. The incidence of adverse reactions in the treatment process was observed, and the clinical efficacy was evaluated. Results:The total effective rate of observation group was 79.3% (42/53), and that of control group was 61.5% (32/52), where the difference between the two groups was statistically significant ( χ2=3.955, P=0.047). After treatment, the scores of lumbar and knee soreness, lumbar and spinal pain, lower limb flaccidity, walking difficulty and the VAS scores in the observation group were significantly lower than those in the control group ( t=21.956, 20.472, 18.591, 13.439, 19.594, 9.035, P<0.001). After treatment, the Troch [(0.70 ± 0.07) g/cm 2vs. (0.67 ± 0.06) g/cm 2, t=2.356] and Ward’s [(0.57 ± 0.06) g/cm 2vs. (0.510 ± 0.05) g/cm 2, t=5.561] and the bone mineral density of L 1-4 [(0.88 ± 0.07) g/cm 2vs. (0.80 ± 0.05) g/cm 2, t=6.727] in the observation group were significantly higher than those in the control group ( P<0.05). The serum levels of SRC-3 [(0.34 ± 0.08) ng/L vs. (0.27 ± 0.07) ng/L, t=4.768], Bcl-2 [(9.56 ± 2.15) μg/L vs. (8.45 ± 2.07) μg/L, t=1.917] and PGC-1a[(0.33 ± 0.06) g/L vs. (0.25 ± 0.04) g/L, t=8.023] in the observation group were significantly higher than those in the control group ( P<0.05). No serious adverse reactions occurred in both groups during the treatment. Conclusion:Wenshen-Gushu Decoction combined with conventional western medicine shows clinical effect and can reduce clinical symptoms, improve bone mineral density, and upregulate the levels of SRC-3, Bcl-2, PGC-1a for the postmenopausal osteoporosis patients with kidney yang deficiency typea with safety.

Effect of Herbal Medicines in Wuzi Yanzong Wan Processed with Salt-water on Apoptosis of Spermatogenic Cells in Rats / 中国实验方剂学杂志

Objective:To observe the effect of Wuzi Yanzong Wan made of different processed products on the apoptosis of spermatogenic cells in rats with kidney essence deficiency， and explore its protective effect on spermatogenic cells. Method:SD rats were randomly divided into the blank group， model group， whole raw product group， pharmacopoeia group and salt-processed product group， with 8 rats in each group. The kidney essence deficiency model was replicated by giving tripterygium glycoside tablets （the dose of 20 mg·kg^-1）. The flow cytometry （FCM） was used to analysis the apoptosis of spermatogenic cells in testis， the immunohistochemistry （IHC） and Western blot were used to detect the expression levels of B-cell lymphoma-2 （Bcl-2） and Bcl-2 associated X protein （Bax） in the testis. High performance liquid chromatography （HPLC） was used to compare the contents of eight components （chlorogenic acid， ellagic acid， hyperoside， isoquercitrin， verbascoside， astragalin， kaempferol and schisandrin） in Wuzi Yanzong Wan made of different processed products， the mobile phase was composed of acetonitrile （A）-0.4% phosphoric acid aqueous solution （B） for gradient elution （0-5 min， 5%-15%A； 5-10 min， 15%-17%A； 10-25 min， 17%A； 25-35 min， 17%-26%A； 35-60 min， 26%-56%A）， the detection wavelength was set at 254 nm. Result:Compared with the model group， the total apoptosis rate of spermatogenic cells， protein expression of Bax and Bcl-2 in each administration group were improved. Among them， the pharmacopoeia group and salt-processed product group had significant effects （<italic>P</italic><0.01）， and the improvement effect of the pharmacopoeia group and salt-processed product group was significantly better than that of the whole raw product group （<italic>P</italic><0.05）. The contents of chlorogenic acid， hyperoside， isoquercitrin and verbascoside in Wuzi Yanzong Wan were increased after the herbal medicines being processed with salt-water. The content of ellagic acid in the salt-processed product group increased， while it decreased in the pharmacopoeia group. The contents of verbascoside， astragalin， kaempferol and schisandrin in samples from the salt-processed product group were greater than those in samples from the pharmacopoeia group. Conclusion:Wuzi Yanzong Wan may reduce the apoptosis of spermatogenic cells in rat testis by inhibiting the expression of Bax and promoting the expression of Bcl-2， and exert its effect of nourishing kidney and enriching essence. The enhanced anti-spermatogenic effect of Wuzi Yanzong Wan after processing may be related to the changes in chemical composition content after processing.

Clinical Efficacy of Needle Warming Moxibustion Combined with Congrong Duhuosan in Treating Ankylosing Spondylitis with Deficiency of Kidney-Yang Type / 中国实验方剂学杂志

Objective:To observe the efficacy and safety of warm acupuncture combined with Congrong Duhuosan in treating ankylosing spondylitis （AS） with deficiency of kidney-Yang. Method:Totally 186 patients with AS of deficiency of kidney Yang type were randomly divided into observation group and control group, with 93 patients in each group. The control group was treated with meloxicam dispersive tablets combined with Sulfasalazine enteric-coated tablets, while the observation group was treated with warm acupuncture combined with Congrong Duhuosan, with a course of treatment of 63 d. Bath ankylosing spondylitis patient global score (BAS-G), visual analogue score (VAS), bath ankylosing spondylitis disease activity index (BASDAI), bath ankylosing spondylitis functional index (BASFI), physicians global assessment (PGA) and syndrome differentiation of kidney-Yang deficiency syndrome in traditional Chinese medicine （TCM） were observed before and after treatment. Interleukin-17 (IL-17)，interleukin-23 (IL-23) mRNA expression levels, T helper cell 17（Th17） and regulatory cells（Treg）levels and Th17/Treg ratio were detected before and after treatment. Efficacy and incidence of adverse reactions were compared between two groups. Result:There were 7 cases falling off in control group and 1 case in observation group. After treatment, the total effective rate of observation group was 85.87%（79/92）, which was better than 60.47%（52/86）of control group (χ2=6.08，P<0.01). The scores of BAS-G, VAS, BASDAI, BASFI, PGA and TCM kidney-Yang deficiency syndrome in observation group were significantly improved than those in control group (P<0.05, P< 0.01). IL-17 and IL-23 mRNA expression levels, Th17 and Treg levels and Th17/Treg ratio in observation group were significantly improved compared with control group (P<0.01). The incidence of adverse reactions in observation group was significantly lower than that in control group (P<0.01). Conclusion:Warm acupuncture combined with Congrong Duhuosan can effectively relieve pain, inflammatory reaction and other clinical symptoms of patients with kidney-Yang deficiency AS, and restore the activity function of patients with small adverse reactions. Therefore, it is a safe and effective clinical therapy.

Clinical observation on electroacupuncture combined with moxibustion for treatment of urinary incontinence after stroke due to deficiency of kidney-yang / 针刺研究

OBJECTIVE: To observe the clinical effect of electroacupuncture combined with moxibustion in the treatment of urinary incontinence after stroke due to deficiency of kidney-yang. METHODS: Sixty patients with urinary incontinence after stroke due to kidney-yang deficiency were randomly divided into a control group and an observation group，with 30 cases in each group. The patients in the control group were given oral administration of Jingui Shenqi pills and Suoquan capsules. On the basis of the treatment in the control group, the patients in the observation group were given electroacupuncture treatment at the Foot Motor sensory Area on the head, Shenshu(BL23) and Huiyang(BL35), together with moxibustion at Guanyuan(CV4) and Qihai(CV6) once daily. The treatment was conducted 6 times per week and for 2 successive weeks. The average number of daily urinary incontinence and night urinary incontinence, maximum bladder volume, residual bladder urine volume, degree of urinary incontinence and clinical symptoms score of the two groups before and after treatment were observed. And the clinical efficacy of the two groups were compared. RESULTS: After the treatment, compared with those before the treatment, the average numbers of daily and night urinary incontinence of the two groups were significantly reduced (P<0.01), the maximum bladder volume was significantly increased (P<0.05), and the residual urine volume of the bladder was significantly reduced (P<0.05). The number of cases with urinary incontinence degree Ⅰ and Ⅱ increased (P<0.05), and the score of clinical symptoms of urinary incontinence was significantly reduced (P<0.05). After the treatment, compared with the control group, the number of daily and night urinary incontinence in the observation group decreased (P<0.05), the maximum bladder volume increased (P<0.05), the residual bladder urine volume decreased (P<0.05), and the number of cases with urinary incontinence degree Ⅰ and Ⅱ increased (P<0.05), the clinical symptom score of urinary incontinence was significantly reduced (P<0.05). The total effective rates of the control group and the observation group were 73.3% (22/30) and 93.3% (28/30) respectively, and the effective rate of the observation group was higher than that of the control group (P<0.05). CONCLUSION: The combination of electroacupuncture, moxibustion and traditional Chinese medicine is effective in treating urinary incontinence after stroke due to deficiency of kidney-yang.

Observations on the effect of thunder-fire moxibustion with meridian point on deficiency of kidney and spleen, and blood stasis in chronic kidney diseases stage three / 中国实用护理杂志

Objective@#To observe clinical effect of thunder-fire moxibustion with meridian point, including lumbar spinal cordacupoints of bladder meridian and abdominal acupoints of Ren channel, which treating on deficiency of kidney and spleen and blood stasis in chronic kidney diseases (CKD) stage 3.@*Methods@#Totally 60 patients were randomly divided into treatment and control group. 30 patients in control were treated with blood pressure and lipid adjustment, Chinese medicine and nutritional therapy. 30 patients in treatment group were treated with thunder-fire moxibustion with meridian point based on control. A moxibustion-treated course has 2 weeks, patients were treated every other day. Observe blood urea nitrogen (Bun) , creatinine (Scr) , glomerular filtration rate (eGFR) , Chinese medicine syndrome and quantitative score, as well as clinical efficacy.@*Results@#After 4 weeks of intervention, the TCM syndrome score [8 (4,10)] in the treatment group was significantly lower than that in the control group [12 (10,14)], with a statistically significant difference (Z=-3.706, P = 0.000); in the quantitative score of symptom classification, the patients in the treatment group were significantly lower than those in the control group [2 (0,2)], chills [2 (2,2)], fatigue [2 (2,4)], the difference was statistically significant (Z=-2.625, -3.593, -4.609, P < 0.01). The frequency of nocturia in the treatment group was significantly higher than that in the control group (Z=-1.989, P < 0.05), while that in the treatment group was not significantly better than that in the control group (P > 0.05). In terms of clinical efficacy, the total effective rate of the treatment group was 93.3% (28/30), which was better than73.3% (22/30) of the control group (χ2= 38.19, P = 0.000).@*Conclusion@#Thunder-fire moxibustion with meridian point improve deficiency of kidney and spleen and blood stasis of patients in CKD stage3, such as weak waist, cold chills, poor appetite, weakness and nocturia, as well as ameliorate renal function.

Effect of Wubi Shanyao pills on sexual function in mice with kidney-yang-deficiency induced by hydrocortisone / 浙江大学学报·医学版

OBJECTIVE@#To investigate the effect of Chinese medicine Wubi Shanyao pills on sexual function of kidney-yang-deficiency mice induced by hydrocortisone.@*METHODS@#Male Kunming mice were injected with hydrocortisone for 10 days to prepare the kidney-yang-deficiency model, and administrated with Wubi Shanyao pills (0.91, 1.82, 2.73 g/kg) for 9 weeks. The general behaviors of mice (autonomous activity, grasping power) were observed; sexual behaviors (capture, straddle, ejaculation frequency and incubation period) of mice were detected by mating experiment. The serum levels of cortisol, adrenocorticotropic hormone (ACTH), luteinizing hormone (LH), follicle stimulating hormone (FSH), estradiol (E@*RESULTS@#Wubi Shanyao pills increased the number of independent activities, grasping power, capture frequency of model mice and shortened the capture latency (all @*CONCLUSIONS@#Wubi Shanyao pills can improve the sexual function of mice with kidney-yang-deficiency induced by hydrocortisone, which may be related to regulating the hypothalamus-pituitary-adrenal axis (HPA axis), promoting the proliferation of testicular cells, and inhibiting cell apoptosis.

Experience of Treating Adolescent Polycystic Ovary Syndrome of Professor LU Su / 浙江中医药大学学报

[Objective]The paper summarizes the clinical experience of Professor LU Su for treating adolescent polycystic ovary syndrome. [Methods] By learning from Professor LU Su, analyse the clinical cases, sum up the knowledge of etiology and pathogenesis, treatment characteristics and cases. [Results] Professor LU Su thought that the main etiological and pathogenesis of adolescent polycystic ovary syndrome was the deficiency of the kidney in the root and phlegm-dampness in the tip, which was the further development of kidney deficiency, resulting in the lack of materal foundation and impetus that cause the growth of the egg and the menses. In treatment, LU Su is good at syndrome differentiation and treatment, invigorating the kidney and resolving phlegm-dampness regulating menstrual cycle, integrating therapy of traditional Chinese and western medicine and combining medication with psychotherapy.[Conclusion] Therapies of Professor LU Su on treating adolescent polycystic ovary syndrome have significant effect.

Professor GAO Yueping's Experience in Treating Sequelae of Pelvic Inflammatory Disease Caused by Shenxu Xueyu / 浙江中医药大学学报

[Objective] To summarize the clinical experience of Professor GAO's treatment of sequelae of pevic inflammatory disease(SPID), and to provide a new approach for treating SPID. [Methods]By listening to Professor GAO's understanding about the etiology and pathogenesis, clinical manifestations, treatment principle of SPID, reading the relevant literature, collecting typical cases to sum up the characteristics of SPID.[Result]With years of clinical experience, Professor GAO proposes the theory about pathogenesis of Shenxu Xueyu,the deficiency of shenyang is most common, Professor GAO 's Wenyang Huayu decoction can Yiqi Wenyang, Huayu Zhitong effectively, in order to treat both causes and symptoms with tonification and purgation in combination, she also tells the patients to pay more attention to the prevention and nursing of the disease.[Conclusion]According to dialectical treatment of TCM,Professor GAO has unique theory and characteristics in treating SPID with remarkable clinical effect, it has high application and promotion value.

Experience of Treating Menopausal Syndrome of Professor Lu Su / 浙江中医药大学学报

Objective]The paper summarizes the clinical experience of Professor Lu Su for treating menopausal syndrome.[Methods] By learning from Professor Lu Su, sum up the knowledge of etiology and pathogenesis, treatment characteristics and analyze cases. [Results]Professor Lu Su thought that the main etiological and pathogenesis of menopausal syndrome was that deficiency of the kidney-yin and hyperactivity of heart and liver fire. The kidney water cannot aid heart, the occurrence of heart(including liver) fire too strong, the heart is not calm, leading to hot flashes sweating, upset insomnia and other symptoms of menopausal syndrome. Therefore, she made a total therapeutic principle of invigoration of the kidney and nourishment of Yin, purging heart fire and soothing the nerves. Besides, the article introduces prescribing characteristics of other lesions relating to menopausal syndrome, such as blood stasis, phlegm, yang deficiency and disharmony between spleen and stomach. In treatment, Lu Su is good at syndrome differentiation and treatment, prescribing refinedly and simply, combining medication with psychotherapy. [Conclusion]Therapies of Professor Lu Su on treating menopausal syndrome have significant effect.

<b>A Case of Trigeminal Trophic Syndrome Treated by a Combination of Rokumigan and Sansoninto</b> / 日本東洋医学雑誌

A 7 year-old male patient who suffer from trigeminal trophic syndrome following herpes zoster was treated with a combination of rokumigan and sansoninto. We conclude that the pathogeneses in this case were excessive rises in liver yang and liver wind caused by yin deficiencies of the kidneys and liver, and that these were ameliorated by the effect of the rokumigan and sansoninto combination in nourishing kidney yin, clearing away heat-evil, and eliminating restlessness.

The influence of kidney-yang deficiency on rat's gastric antrum and small intestinal mucosa tissue microstructure change / 国际中医中药杂志

Objective To research the influence kidney-yang deficiency on rat' s gastric antrum and small intestinal mucosa tissue microstructure' s change.Methods The rats were cut off bilateral adrenal three-quarters to establish the model rats with deficiency of kidney-yang,which were randomly divided into a model group and a Shenfu decoction group at 4 weeks after operation.The sham operation group was the same as model group except without cutting rats bilateral adrenal and setting as a control group.The Shenfu decoction group was given suspension liquid of Shenfu decoetion by lavage,and both the control group and model group were given physiological saline of the same capacity for 4 weeks.Each rat gastric antrum and small intestinal mucosa's organization structure by conventional HE slice was observed.Results ① The sham operation group rats' gastric and intestinal mucosa were pink,shiny and elastic by macroscopic observation.Compared with the sham operation group,the gastric and intestinal mucosa of the model group became thinner,dimmer and less elastic.Compared with the model group,the gastric and intestinal mucosa of the Shenfu decoction group were a bit thicker,shinier and more elastic.② The results of the light microscope showed that:The gastric antrum and intestinal mucosa's tissue structure were normal.Compared with the sham operation group,the antrum mucosa tissue's cell gap of the model group became larger,besides the mucous glands had different degrees of alignment disorders,and a large number of vacuolar degeneration Appeared.The intestinal mucosa tissue villus edge was irregular,partial shedding of the epithelium fell offand deleted.Compared with the model group,the antrum mucosa tissue's cell gap of the Shenfu decoction group became smaller,the mucous glands cells arranged more regular,and the vacuolar degeneration had reduced.The intestinal mucosa tissue villus edge was more regular,and intestinal mucosal epithelial tissue was more complete.Conclusion The pathological changes were emerged in kidney-yang deficiency rat models.

The effect of Compound Xuanju Capsule and Prednisone on male infertility / 国际中医中药杂志

Objective To observe the curative effect of the combined action of Compound Xuanju Capsule and Prednisone on oligospermatism and antisperm antibody due to yang deficiency of kidney. Methods 136 patients were randomly divided into two groups: a treated group, which was taken Compound Xuanju Capsule and Prednisone, and a control group, which was taken Prednisone and Tamoxifen Citrate Tablet. Both groups received a 3-month therapy. The conditions of semen and antisperm antibody between the two groups were observed before and after the treatment. Results The total effective rate of the treated group was 90.94% (61 in 67 cases), and in which 11 cases were pregnant (16.41%). While the total effective rate of the control group was 63.07% (41 in 65 cases), and in which 2 cases were pregnant (3.08%).The clinical efficacy of the treated group was better than that of the control group. (P＜0.05) After the treatment, sperm density and motility of two groups were both evidently increased, (P＜0.05, P＜0.01) and the treated group increased much more obviously (P＜0.05). Conclusion The combined action of Compound Xuanju Capsule and Prednisone can improve the curative effect on the treatment for male infertility from oligospermatism and antisperm antibody due to yang deficiency of kidney.

The Status and Considerations of Study on Kidney-Yang Deficiency / 浙江中医药大学学报

With the development of Chinese medical research,it becomes an important problem how to scientifically explain syndrome in Chinese medicine.In this paper,the progress on deficiency of kidney yang is reviewed from endocrine,immune function and energy metabolism,and it is preferred that the technology of genome and proteome be used to study the deficiency of kidney yang.

Experimental Study of the Correlation of Deficiency of Kidney-Yang and Stagnation of Blood / 中国中医药信息杂志

Objective To study the change law between deficiency of kidney-Yang and stagnation of blood, and supply a study evidence for the therapy method of invigorate the kidney and promoting blood flow. Method 37 Wistar rats which were made of the model of deficiency of kidney-Yang, were randomly divided into three groups:control group, model group and treatment group. Changes including appearance, general objective sign, organ weight, microcirculation and blood rheology were recorded. Result Phenomenons, including rare fur, depression, lazy, duck stools and purpl dim in micrangium micrangium, occurred in model group. The weight of adrenal body and seminal vesicle and the contents of serum andrusol in model group were lower than those in control group. And in model group, significant increasing TC and LDL-C and decreasing HCL-C were observed. Conclusion Deficiency of kidney-Yang is associated with stagnation of blood, and the time delay of stagnation of blood can lead deficiency of kidney-Yang.

Discussion on Alzheimer disease pathology in Chinese medicine / 中华中医药杂志

Doctors of past dynasties already understood deficiency of kidney essence,phlegm and blood stasis were important for dementia.As the increasing recognition about Alzheimer disease and the offer of 'toxin damaging brain vessel' hypothesis,we thought ?-Amyloid,the pathology of Alzheimer disease,had the character of damaging brain and marrow,so it belonged to internal toxin,internal toxin damaging brain and marrow was also the pathology of the disease.Deficiency of kidney essence was the precondition,insufficient brain and marrow and internal toxin damaging brain and marrow were the two causes of brain and marrow consumption and spirit dysfunction,which were the pathology of Alzheimer disease in Chinese medicine.

Construction and application of heart-kidney centric theory / 中华中医药杂志

This article put forward the ‘heart-kidney centric theory’ on basis of analyzing the physiologic function, pathomechanism, onset rules and measures of prevention and treatment of cardio-cerebrovascular disease and degenerative disease induced by kidney deficiency. According the new theory, a new TCM drug, the Tongmai Qiangshen Wine, was developed.In human body, kidney produces essential qi and produces energy source for human body; heart transports the essential qi and is the physiologic mechanism of transport of energy source. Deficiency of essential qi will result in stasis of blood vessel, on the other hand, stasis of blood vessel can cause defi ciency of essential qi. The opposition, mutual rooting, waxing and waning, mutual convertibility of them reflect the development rules of cardio-cerebrovascular disease and degenerative disease induced by kidney deficiency, and determin the vital process of birth, growth, adult, aging and death. The construction of heart-kidney centric theory and the development and application of Tongmai Qiangshen Wine not only provide new theory and model for prevention and treatment of cardio-cerebrovascular disease and degenerative disease induced by kidney deficiency, but also provide new thoughts for preparation of new TCM drugs from aspects of how to maintain the feature of TCM, how to inherit and create on basis of combing holism with disease, principle, method, medicines.

Anti-aging Effect of Polysaccharides from Crude and Processed Cornus Officinalis on Mice with Deficiency of Kidney / 中药新药与临床药理

Objective To compare the anti-aging effect of polysaccharides from crude and processed Cornus Officinalis on the mice with deficiency of kidney.Methods The mice were given the drugs by gastric perfusion. The effect of polysaccharides on the time of weight-loading swimming, anti-hypoxia time , and serum SOD activity and MDA content in mice with kidney deficiency was observed. Results Compared to the crude Cornus Officinalis, the polysaccharides from the processed product can markedly prolong the time of weight-loading swimming, anti-hypoxia time, promote the activity of SOD and decrease the content of MDA. Conclusions Polysaccharides from Cornus Officinalis have certain anti-aging effect on the mice with deficiency of kidney, and the effect of the processed is superior to that of the crude.

Study on Correlativity of Senile Dementia of Type of Deficiency of Kidney Essence with Polymorphophism of APOE Gene / 中医杂志

Objective:To explore correlativity of senile dementia of deficiency of kidney essence with polymorphophism of APOE gene. Methods:41 cases of senile dementia and 85 healthy aging persons were divided into 3 TCM syndrome types:syndrome of deficiency of kidney essence,syndrome of orifices confused by phlegm,and syndrome of obstruction of blood stasis in collaterals. DNA in white blood cells was isolated by NaI,and APOE gene was detected by milti-ARMs PCR,and then correlative analysis was made.Results:(1)There was a significant difference between the dementia group and the control group in the gene type ?3/ ?3(P1.(3)There was significant correlativity between syndrome of deficiency of kidney essence and APOE ?4 allelic gene(P