Curative effect of Wenshen-Gushu Decoction combined with conventional western medicine for the postmenopausal osteoporosis patients with kidney yang deficiency type / 国际中医中药杂志

Objective:To investigate the clinical effect of Wenshen-Gushu Decoction combined with conventional western medicine on postmenopausal osteoporosis patients with kidney yang deficiency type. Methods:A total of 105 postmenopausal osteoporosis patients admitted in Huxi Hospital Affiliated to Jining Medical College from January 2017 to December 2019 were selected. According to the random number table method, they were divided into the control group (52 cases) and observation group (53 cases). The control group was treated with conventional western medicine, and the observation group was treated with self-made Wenshen-Gushu Decoction on the basis of the control group. Both groups were treated for 8 weeks. The TCM Symptom Score, Visual Analogue Scale (VAS), bone mineral density of troch (Troch), bone mineral density of ward’s triangle (ward’s), bone mineral density of L 1-4 and serum levels of steroid hormone receptor coactivator-3 (SRC-3), B-cell lymphoma gene-2 (Bcl-2) and transcription element coactivator (PGC-1a) were compared between two groups before and after treatment. The incidence of adverse reactions in the treatment process was observed, and the clinical efficacy was evaluated. Results:The total effective rate of observation group was 79.3% (42/53), and that of control group was 61.5% (32/52), where the difference between the two groups was statistically significant ( χ2=3.955, P=0.047). After treatment, the scores of lumbar and knee soreness, lumbar and spinal pain, lower limb flaccidity, walking difficulty and the VAS scores in the observation group were significantly lower than those in the control group ( t=21.956, 20.472, 18.591, 13.439, 19.594, 9.035, P<0.001). After treatment, the Troch [(0.70 ± 0.07) g/cm 2vs. (0.67 ± 0.06) g/cm 2, t=2.356] and Ward’s [(0.57 ± 0.06) g/cm 2vs. (0.510 ± 0.05) g/cm 2, t=5.561] and the bone mineral density of L 1-4 [(0.88 ± 0.07) g/cm 2vs. (0.80 ± 0.05) g/cm 2, t=6.727] in the observation group were significantly higher than those in the control group ( P<0.05). The serum levels of SRC-3 [(0.34 ± 0.08) ng/L vs. (0.27 ± 0.07) ng/L, t=4.768], Bcl-2 [(9.56 ± 2.15) μg/L vs. (8.45 ± 2.07) μg/L, t=1.917] and PGC-1a[(0.33 ± 0.06) g/L vs. (0.25 ± 0.04) g/L, t=8.023] in the observation group were significantly higher than those in the control group ( P<0.05). No serious adverse reactions occurred in both groups during the treatment. Conclusion:Wenshen-Gushu Decoction combined with conventional western medicine shows clinical effect and can reduce clinical symptoms, improve bone mineral density, and upregulate the levels of SRC-3, Bcl-2, PGC-1a for the postmenopausal osteoporosis patients with kidney yang deficiency typea with safety.

Clinical Efficacy of Needle Warming Moxibustion Combined with Congrong Duhuosan in Treating Ankylosing Spondylitis with Deficiency of Kidney-Yang Type / 中国实验方剂学杂志

Objective:To observe the efficacy and safety of warm acupuncture combined with Congrong Duhuosan in treating ankylosing spondylitis （AS） with deficiency of kidney-Yang. Method:Totally 186 patients with AS of deficiency of kidney Yang type were randomly divided into observation group and control group, with 93 patients in each group. The control group was treated with meloxicam dispersive tablets combined with Sulfasalazine enteric-coated tablets, while the observation group was treated with warm acupuncture combined with Congrong Duhuosan, with a course of treatment of 63 d. Bath ankylosing spondylitis patient global score (BAS-G), visual analogue score (VAS), bath ankylosing spondylitis disease activity index (BASDAI), bath ankylosing spondylitis functional index (BASFI), physicians global assessment (PGA) and syndrome differentiation of kidney-Yang deficiency syndrome in traditional Chinese medicine （TCM） were observed before and after treatment. Interleukin-17 (IL-17)，interleukin-23 (IL-23) mRNA expression levels, T helper cell 17（Th17） and regulatory cells（Treg）levels and Th17/Treg ratio were detected before and after treatment. Efficacy and incidence of adverse reactions were compared between two groups. Result:There were 7 cases falling off in control group and 1 case in observation group. After treatment, the total effective rate of observation group was 85.87%（79/92）, which was better than 60.47%（52/86）of control group (χ2=6.08，P<0.01). The scores of BAS-G, VAS, BASDAI, BASFI, PGA and TCM kidney-Yang deficiency syndrome in observation group were significantly improved than those in control group (P<0.05, P< 0.01). IL-17 and IL-23 mRNA expression levels, Th17 and Treg levels and Th17/Treg ratio in observation group were significantly improved compared with control group (P<0.01). The incidence of adverse reactions in observation group was significantly lower than that in control group (P<0.01). Conclusion:Warm acupuncture combined with Congrong Duhuosan can effectively relieve pain, inflammatory reaction and other clinical symptoms of patients with kidney-Yang deficiency AS, and restore the activity function of patients with small adverse reactions. Therefore, it is a safe and effective clinical therapy.

Clinical observation on electroacupuncture combined with moxibustion for treatment of urinary incontinence after stroke due to deficiency of kidney-yang / 针刺研究

OBJECTIVE: To observe the clinical effect of electroacupuncture combined with moxibustion in the treatment of urinary incontinence after stroke due to deficiency of kidney-yang. METHODS: Sixty patients with urinary incontinence after stroke due to kidney-yang deficiency were randomly divided into a control group and an observation group，with 30 cases in each group. The patients in the control group were given oral administration of Jingui Shenqi pills and Suoquan capsules. On the basis of the treatment in the control group, the patients in the observation group were given electroacupuncture treatment at the Foot Motor sensory Area on the head, Shenshu(BL23) and Huiyang(BL35), together with moxibustion at Guanyuan(CV4) and Qihai(CV6) once daily. The treatment was conducted 6 times per week and for 2 successive weeks. The average number of daily urinary incontinence and night urinary incontinence, maximum bladder volume, residual bladder urine volume, degree of urinary incontinence and clinical symptoms score of the two groups before and after treatment were observed. And the clinical efficacy of the two groups were compared. RESULTS: After the treatment, compared with those before the treatment, the average numbers of daily and night urinary incontinence of the two groups were significantly reduced (P<0.01), the maximum bladder volume was significantly increased (P<0.05), and the residual urine volume of the bladder was significantly reduced (P<0.05). The number of cases with urinary incontinence degree Ⅰ and Ⅱ increased (P<0.05), and the score of clinical symptoms of urinary incontinence was significantly reduced (P<0.05). After the treatment, compared with the control group, the number of daily and night urinary incontinence in the observation group decreased (P<0.05), the maximum bladder volume increased (P<0.05), the residual bladder urine volume decreased (P<0.05), and the number of cases with urinary incontinence degree Ⅰ and Ⅱ increased (P<0.05), the clinical symptom score of urinary incontinence was significantly reduced (P<0.05). The total effective rates of the control group and the observation group were 73.3% (22/30) and 93.3% (28/30) respectively, and the effective rate of the observation group was higher than that of the control group (P<0.05). CONCLUSION: The combination of electroacupuncture, moxibustion and traditional Chinese medicine is effective in treating urinary incontinence after stroke due to deficiency of kidney-yang.

Effect of Wubi Shanyao pills on sexual function in mice with kidney-yang-deficiency induced by hydrocortisone / 浙江大学学报·医学版

OBJECTIVE@#To investigate the effect of Chinese medicine Wubi Shanyao pills on sexual function of kidney-yang-deficiency mice induced by hydrocortisone.@*METHODS@#Male Kunming mice were injected with hydrocortisone for 10 days to prepare the kidney-yang-deficiency model, and administrated with Wubi Shanyao pills (0.91, 1.82, 2.73 g/kg) for 9 weeks. The general behaviors of mice (autonomous activity, grasping power) were observed; sexual behaviors (capture, straddle, ejaculation frequency and incubation period) of mice were detected by mating experiment. The serum levels of cortisol, adrenocorticotropic hormone (ACTH), luteinizing hormone (LH), follicle stimulating hormone (FSH), estradiol (E@*RESULTS@#Wubi Shanyao pills increased the number of independent activities, grasping power, capture frequency of model mice and shortened the capture latency (all @*CONCLUSIONS@#Wubi Shanyao pills can improve the sexual function of mice with kidney-yang-deficiency induced by hydrocortisone, which may be related to regulating the hypothalamus-pituitary-adrenal axis (HPA axis), promoting the proliferation of testicular cells, and inhibiting cell apoptosis.

Professor GAO Yueping's Experience in Treating Sequelae of Pelvic Inflammatory Disease Caused by Shenxu Xueyu / 浙江中医药大学学报

[Objective] To summarize the clinical experience of Professor GAO's treatment of sequelae of pevic inflammatory disease(SPID), and to provide a new approach for treating SPID. [Methods]By listening to Professor GAO's understanding about the etiology and pathogenesis, clinical manifestations, treatment principle of SPID, reading the relevant literature, collecting typical cases to sum up the characteristics of SPID.[Result]With years of clinical experience, Professor GAO proposes the theory about pathogenesis of Shenxu Xueyu,the deficiency of shenyang is most common, Professor GAO 's Wenyang Huayu decoction can Yiqi Wenyang, Huayu Zhitong effectively, in order to treat both causes and symptoms with tonification and purgation in combination, she also tells the patients to pay more attention to the prevention and nursing of the disease.[Conclusion]According to dialectical treatment of TCM,Professor GAO has unique theory and characteristics in treating SPID with remarkable clinical effect, it has high application and promotion value.

The influence of kidney-yang deficiency on rat's gastric antrum and small intestinal mucosa tissue microstructure change / 国际中医中药杂志

Objective To research the influence kidney-yang deficiency on rat' s gastric antrum and small intestinal mucosa tissue microstructure' s change.Methods The rats were cut off bilateral adrenal three-quarters to establish the model rats with deficiency of kidney-yang,which were randomly divided into a model group and a Shenfu decoction group at 4 weeks after operation.The sham operation group was the same as model group except without cutting rats bilateral adrenal and setting as a control group.The Shenfu decoction group was given suspension liquid of Shenfu decoetion by lavage,and both the control group and model group were given physiological saline of the same capacity for 4 weeks.Each rat gastric antrum and small intestinal mucosa's organization structure by conventional HE slice was observed.Results ① The sham operation group rats' gastric and intestinal mucosa were pink,shiny and elastic by macroscopic observation.Compared with the sham operation group,the gastric and intestinal mucosa of the model group became thinner,dimmer and less elastic.Compared with the model group,the gastric and intestinal mucosa of the Shenfu decoction group were a bit thicker,shinier and more elastic.② The results of the light microscope showed that:The gastric antrum and intestinal mucosa's tissue structure were normal.Compared with the sham operation group,the antrum mucosa tissue's cell gap of the model group became larger,besides the mucous glands had different degrees of alignment disorders,and a large number of vacuolar degeneration Appeared.The intestinal mucosa tissue villus edge was irregular,partial shedding of the epithelium fell offand deleted.Compared with the model group,the antrum mucosa tissue's cell gap of the Shenfu decoction group became smaller,the mucous glands cells arranged more regular,and the vacuolar degeneration had reduced.The intestinal mucosa tissue villus edge was more regular,and intestinal mucosal epithelial tissue was more complete.Conclusion The pathological changes were emerged in kidney-yang deficiency rat models.

The Status and Considerations of Study on Kidney-Yang Deficiency / 浙江中医药大学学报

With the development of Chinese medical research,it becomes an important problem how to scientifically explain syndrome in Chinese medicine.In this paper,the progress on deficiency of kidney yang is reviewed from endocrine,immune function and energy metabolism,and it is preferred that the technology of genome and proteome be used to study the deficiency of kidney yang.

Experimental Study of the Correlation of Deficiency of Kidney-Yang and Stagnation of Blood / 中国中医药信息杂志

Objective To study the change law between deficiency of kidney-Yang and stagnation of blood, and supply a study evidence for the therapy method of invigorate the kidney and promoting blood flow. Method 37 Wistar rats which were made of the model of deficiency of kidney-Yang, were randomly divided into three groups:control group, model group and treatment group. Changes including appearance, general objective sign, organ weight, microcirculation and blood rheology were recorded. Result Phenomenons, including rare fur, depression, lazy, duck stools and purpl dim in micrangium micrangium, occurred in model group. The weight of adrenal body and seminal vesicle and the contents of serum andrusol in model group were lower than those in control group. And in model group, significant increasing TC and LDL-C and decreasing HCL-C were observed. Conclusion Deficiency of kidney-Yang is associated with stagnation of blood, and the time delay of stagnation of blood can lead deficiency of kidney-Yang.